Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	2,958,523	C→T	A428V (GCA→GTA)	PP_2589 →	Aldehyde dehydrogenase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	2,958,523	0	C	T	100.0%	116.5 / NA	36	A428V (GCA→GTA)	PP_2589	Aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/26);  total (10/26)

CGCGCCGGGGCCATTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGG  >  AE015451/2958252‑2958816                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                      cgcgCCGGGGCCATTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAAcc                                                                                                                                                                                                                                                                          >  1:312272/1‑301 (MQ=255)               ggtgactggcgcgccgaccgggctcgtCGAGGCAGCCATTGTGGGTCGCGGGCAAGAGGGCTCGCGGTTGGTGTGTGGCGGCCGCCGCGTGACCATCGAAAGTACGGACAACTACATCGAGCCGACCGTTCTTGCCGCTGTCGAGGGCCGGATGGGCCTGGCGCGCGAGCAGGTGTTCGGCCCGGTACTGGCGGTCAGCTCCTTCGGCACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTa                                                                                                                                                                                                                                                            <  2:510434/275‑1 (MQ=255)                gTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACACCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACCGCATCTCTTGCCTGGTAGTCTCGGTGTAGAGCGACGAATTCAAACAGGTGCACCggggag                                                                                                                                                                                                                                                           >  1:642523/1‑298 (MQ=255)                             aGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCTGTACTGGCGGTCAGCGCCTTCGACACGGAAGACGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGAAGCCTTCCACCAGGTGCACCGGGGAGCGCGGGCGtttca                                                                                                                                                                                                                                              >  2:750545/1‑298 (MQ=255)                                   gCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGGGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCgg                                                                                                                                                                                                                                        <  1:690664/301‑1 (MQ=255)                                       cATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGGGCGCCTGGCCAACGACAGCGTCTATGGCCTGGTAGACTCGGTGTGGAGCGACGACTTCAACCAGGTGAACCGGGTAGAGCGGGCGTTGAAAGCCGGTAcc                                                                                                                                                                                                                                    >  2:339748/1‑301 (MQ=255)                                                    aTTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAaca                                                                                                                                                                                                                       <  1:419688/301‑1 (MQ=255)                                                                 ggCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGCGGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCt                                                                                                                                                                                                          <  2:243637/301‑1 (MQ=255)                                                                   cAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTAACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGGTGCGCTgg                                                                                                                                                                                                        <  1:733033/301‑1 (MQ=255)                                                                     gggtggctgcggATTGGTGTGTGTCGGCCGGCGACTGACCACCGAGGGTTCGGACACCTACATCGAGCCTACCATTTTTGCCGGTGTCGAGGGCGGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGCTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTCGCCTCGGTGTGGAGCGACGACTTCAACCGGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAt                                                                                                                                                                                                      <  2:704564/290‑1 (MQ=255)                                                                     agagGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGAt                                                                                                                                                                                                      <  1:535744/301‑1 (MQ=255)                                                                         gggcggggATTGGGGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGa                                                                                                                                                                                                  <  2:583068/294‑1 (MQ=255)                                                                                gATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGcc                                                                                                                                                                                           <  1:161187/301‑1 (MQ=255)                                                                                 aTTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGGGTTGAAAGCCGGTCACGTATCGGTGAACCCAGTCGATGCGGTGGATGTGACCGTGccc                                                                                                                                                                                          >  2:192158/1‑300 (MQ=255)                                                                                                       cTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCgg                                                                                                                                                                    >  1:595111/1‑301 (MQ=255)                                                                                                                          aCAACTACATCGGGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTc                                                                                                                                                 <  1:192238/301‑1 (MQ=255)                                                                                                                             agtacctCGAGCCGTCCTGTTTGGCCGGTGTCGTGCGACGCATGCGCCTGGCGCGCCAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACGTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACt                                                                                                                                              <  1:655717/295‑1 (MQ=255)                                                                                                                                                     ccGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGTTGGATGTGACGGTGCCGGTCGGTGGGGGCAAGCAGGCGGGGCTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGcc                                                                                                                      >  1:419251/1‑300 (MQ=255)                                                                                                                                                       ggTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCACGCAGTCGGGGTTTGGCGGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGccac                                                                                                                    >  2:511024/1‑298 (MQ=255)                                                                                                                                                         tgtCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTc                                                                                                                  >  1:741424/1‑301 (MQ=255)                                                                                                                                                                 gCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGaccac                                                                                                          <  1:750860/301‑1 (MQ=255)                                                                                                                                                                                ggcgcgAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTgcg                                                                                           <  1:286707/300‑1 (MQ=255)                                                                                                                                                                                cgcgcgAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTgcg                                                                                           <  1:511232/301‑1 (MQ=255)                                                                                                                                                                                          ggTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGt                                                                                 <  1:383726/301‑1 (MQ=255)                                                                                                                                                                                              ttCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTg                                                                             <  1:648448/301‑1 (MQ=255)                                                                                                                                                                                                     cGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTg                                                                      >  1:137029/1‑301 (MQ=255)                                                                                                                                                                                                         tgtggcggtcgttgcCTTCGACACGGAAGTATAAGCCGTGCGCCGGTCCAACGACAGCTTCAATGGCCTGGTAGCCTCGGTGTAGAGCGACGACTTCACCCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACcg                                                                  <  2:402117/288‑1 (MQ=255)                                                                                                                                                                                                         actgacgggcagccctttggccacGGAAGAAGAAGCCGGGCGGCTGGCCAACGGCAGCATCGATGGCGTGGTAGCGTCGGTGTGGAGCGACGACTTAAACCCGGTGCACCGGGTGGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACGGTCGATGCGCTGAATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACcg                                                                  <  2:419086/280‑1 (MQ=255)                                                                                                                                                                                                         aCTGGCGGTCAGCGCCTCCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCGTCTATGGCCTGGTAGCCGCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACcg                                                                  <  2:136970/301‑1 (MQ=255)                                                                                                                                                                                                                 tCAGCGACTTGGACACGAGAGAAGAAGCCTTGCGGCTGGCAAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACTACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAGAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCt                                                          <  2:119732/301‑1 (MQ=255)                                                                                                                                                                                                                      gcCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTc                                                     <  2:312153/301‑1 (MQ=255)                                                                                                                                                                                                                                       agaagCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCTGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTcc                                    <  1:339875/301‑1 (MQ=255)                                                                                                                                                                                                                                             cGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAgg                              <  1:30534/301‑1 (MQ=255)                                                                                                                                                                                                                                                                 gCATCTATGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTTAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGt          <  1:346347/301‑1 (MQ=255)                                                                                                                                                                                                                                                                       aTGGCCTGGTAGCCTCGGTGTGGAGCGACGACTTCAACCAGGGGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCt    <  2:594861/301‑1 (MQ=255)                                                                                                                                                                                                                                                                         ggCCTGGTAGCCTCGGGGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTgg  <  1:245439/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                      CGCGCCGGGGCCATTGTCGATGCCGGGCAGACCGGCCGCATCGAGGCCGCCATTGTGCGTGCCGGGCAAGAGGGCGCGCGATTGGTGTGTGGCGGCCGACGCCTGACCATCGAAGGTTCGGACAACTACATCGAGCCGACCATTTTTGCCGGTGTCGAGGGCCGCATGAGCCTGGCGCGCGAGGAGGTGTTCGGCCCGGTACTGGCGGTCAGCGCCTTCGACACGGAAGAAGAAGCCGTGCGCCTGGCCAACGACAGCATCTATGGCCTGGCAGCCTCGGTGTGGAGCGACGACTTCAACCAGGTGCACCGGGTAGCGCGGGCGTTGAAAGCCGGTACCGTATCGGTGAACACAGTCGATGCGCTGGATGTGACGGTGCCGTTCGGTGGGGGCAAGCAGTCGGGGTTTGGCCGCGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGTCTGAGGGTTGTGCTGG  >  AE015451/2958252‑2958816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑