Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,741,272	A→G	intergenic (+509/+244)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,741,272	0	A	G	100.0%	153.8 / NA	43	intergenic (+509/+244)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/14);  total (29/14)

TTGCCACCCGAACAAAACCCTTCTCTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCT‑CCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATCCTT  >  AE015451/4741048‑4741495                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 ttGCCACCAGAACAAAACTCTTCTCTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCAAGGAGTCACGGGGCGTCACAGGCCATGGCGTATGGAACTGGGTATAGGTCCAGTCCTGGTCACCTTCGTGCTTGAGCAG‑ATT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGACAGGCCTTGTACtt                                                                                                                                                      >  2:133271/1‑301 (MQ=255)           aaCAAAACCCTTCTCTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGTTCACCTTCGTGCTTGAG‑CA‑GCT‑TCTGCGACTGGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCCCGACCTTGCCCAGCGGCGCCTTGATCACGGTTACACCGCCGTAGGCCTTGGATTTCGACCCGGcc                                                                                                                                            >  2:513241/1‑301 (MQ=255)             cAAAACCCTTCTCTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAc                                                                                                                                          >  1:701562/1‑301 (MQ=255)             cAAAACCCTTCTCTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAc                                                                                                                                          >  1:62138/1‑301 (MQ=255)                         ctTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCATGGTCACACCGCGATAGGCCTTCTACTTGGACCCGGCCACCTCACTGAgggg                                                                                                                              >  2:348204/1‑300 (MQ=255)                             cGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACAcc                                                                                                                          >  1:729443/1‑301 (MQ=255)                                 aGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGTTCACACCGCGATAGGCCTTGTACTTGGACCCGCCCACCTCACTGAGGGACACCTTgc                                                                                                                      >  2:535358/1‑300 (MQ=255)                                   gTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGCGTATAGGTCCAGCTCTGCTCACCTTCGTGCTTGAG‑AAGCTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCCGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCCCGGTCACACCTCGATAGGCCTGGTACTCGGAcccctccaccccctctaggcaccccttgcac                                                                                                                    >  2:365736/1‑273 (MQ=255)                                       gTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCCCTGAGGGACACCTTGATCCcgt                                                                                                                >  2:643308/1‑301 (MQ=255)                                           gCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc                                                                                                            >  1:53593/1‑301 (MQ=255)                                            ctctGCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcg                                                                                                           <  2:62107/301‑1 (MQ=255)                                                tcttTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGTTCACACCGCGATCGGCCTTGGACCTGGACCCTGCCACCTACCTGCGTGACACCTTGATCCcctcctgtgctc                                                                                                       >  2:599166/1‑295 (MQ=255)                                                    tCAGGTTACGGGTCAGGCTGCGCTCCGCCTCCTTGATGGTCGTAATAGGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCATCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTGCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGc                                                                                                   <  2:471668/301‑1 (MQ=255)                                                     cAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGTCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACATCACTGATGGACACCTTGATCCATTCCTCGTCCTCGGcc                                                                                                  >  2:488458/1‑301 (MQ=255)                                                               gTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGTCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCatt                                                                                        >  2:444748/1‑299 (MQ=255)                                                                    gCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCa                                                                                   >  1:332899/1‑301 (MQ=255)                                                                    gCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCa                                                                                   <  1:271444/301‑1 (MQ=255)                                                                     cTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAg                                                                                  >  1:422122/1‑301 (MQ=255)                                                                          ctCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAg                                                                             <  1:596414/300‑1 (MQ=255)                                                                                ctcctTGATGGTCTTAATGTGCAGCAAGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCcaca                                                                       <  2:421957/301‑1 (MQ=255)                                                                                   ctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGc                                                                    >  1:620289/1‑301 (MQ=255)                                                                                     tGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCTCAGGCCCCAGGCGa                                                                  >  2:699568/1‑301 (MQ=255)                                                                                          ggcGTAATGTGCAGCACGGAATCACGCGGCGGCACCGGCCATGGCGGATTGACCGGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcagca                                                             <  1:713204/299‑1 (MQ=255)                                                                                           tCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcagcag                                                            <  1:599427/301‑1 (MQ=255)                                                                                            cGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCCGGCCCCAGGCgccacccga                                                           >  2:506894/1‑293 (MQ=255)                                                                                             gTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTCCTTGGACCCGGCCACCTCACTGAGGGCCACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGGTTTCAGTCCAGGCCAAAGGCGAaagcataa                                                          >  2:698029/1‑298 (MQ=255)                                                                                              tAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAac                                                         <  1:643580/301‑1 (MQ=255)                                                                                                        caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTTCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCCGTTTTCACCCCAGGCCACAGGCGACCCCCGAACACccccaccc                                               >  1:398242/1‑300 (MQ=255)                                                                                                           ggAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCCGTTTTCAGCCCAGGCCACCGTCGACAGCAGCACACCcaccccacac                                            >  1:4179/1‑301 (MQ=255)                                                                                                                cACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGGCCTCGTCCTTGGCCACTTGCCCGGTTTCAGCCCAGCCCACCGGCGACTGCAAAACACCACCACAACAcagccc                                       >  1:60424/1‑299 (MQ=255)                                                                                                                      gcgTCACCGGCCATGGCGTATTGAACTTGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAAt                                 <  2:478265/301‑1 (MQ=255)                                                                                                                       cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATa                                <  1:513449/301‑1 (MQ=255)                                                                                                                       cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATa                                <  1:535579/301‑1 (MQ=255)                                                                                                                                ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGACCCCGGACACGTTCTCCTGCAGCTCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCGTGTACTGGGACCCGGCCACCTCACGGATGGACACCTTGATCCCTTACTCTTCCTTTGCTACTTGCCAGTTTTCAGCCGCGGCCGAAGGCGGCAGCAGAACACCCACACCACAcagcaagacagcacgcttgaaa                       >  2:492315/1‑284 (MQ=255)                                                                                                                                      cGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGAtctc                 <  2:316398/301‑1 (MQ=255)                                                                                                                                      cGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGAtctc                 <  1:133330/301‑1 (MQ=255)                                                                                                                                          ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCtcatca             <  1:444938/301‑1 (MQ=255)                                                                                                                                             aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGt          >  1:415319/1‑301 (MQ=255)                                                                                                                                             aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGt          >  2:108928/1‑301 (MQ=255)                                                                                                                                                tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTa       >  1:194829/1‑301 (MQ=255)                                                                                                                                                  ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAACAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATc     >  1:453664/1‑301 (MQ=255)                                                                                                                                                   gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATTGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGATTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATATGCTTGAACGATCTCATCATGCTTAtac    >  1:304050/1‑299 (MQ=255)                                                                                                                                                     ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTT‑CTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCACTACGCTTGAACGATCTCATCATGTTTATCCtt  >  1:211213/1‑301 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 TTGCCACCCGAACAAAACCCTTCTCTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCT‑CCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATCCTT  >  AE015451/4741048‑4741495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑