Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	6,013,885	T→C	intergenic (+90/+20)	xpt → / ← PP_5266	Xanthine phosphoribosyltransferase/putative acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	6,013,885	0	T	C	100.0%	115.2 / NA	34	intergenic (+90/+20)	xpt/PP_5266	Xanthine phosphoribosyltransferase/putative acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

CGATGACTTCCTGGCCAACGGCAAAGCCTCCCAAGCGCTGATCTCGATCATCAAGCAGGCAGGTGCCACCGTGGCGGGCCTGGGTATCGTGATCGAGAAGTCGTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCGGATACTCCGGGAACAGCCGCGCATGCTGTGCCCTGATCGCCTC  >  AE015451/6013591‑6014180                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        cGATGACTTCCTGGCCAACGGCAAAGCCTCCCAAGCGCTGATCTCGATCATCAAGCAGGCAGGTGCCACCGTGGCGGGCCTGGGTATCGTGATCGAGAAGTCGTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAAt                                                                                                                                                                                                                                                                                                   >  1:84986/1‑301 (MQ=255)               ccAACGGCAAAGCCTCCCAAGCGCTGATCTCGATCATCAAGCAGGCAGGTGCCACCGTGGCGGGCCTGGGTATCGTGATCGAGAAGTCGTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCttt                                                                                                                                                                                                                                                                                     <  1:250268/301‑1 (MQ=255)                              ccccaGCGCGGATCTCGATCATCAAGCCGGCAGGGGCCACCGTGGCGGGCCTGGGTATCGTGATCGAGAAGTCGTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTgccgcc                                                                                                                                                                                                                                                                      <  2:234268/297‑1 (MQ=255)                                           cgagATCAACAAGGAGGCAAGGGCGACGGTGGCGGGGCGGGGTATCGGGAGAGAGAAGTCGTGCGAGGGCGGGCGGGCCGAACGGGAGAGCCAGGGGTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaaca                                                                                                                                                                                                                                                         <  2:325820/298‑1 (MQ=255)                                                                            gggCCTGGGTATGGTGATCGAGAAGTCGTTCCAGGGCGGCCGGGCGGAACTGGACAGCGAGGGTTACCGCGTTGAATCGCTGGCCCGGTTGAAGTCGCTGGAAGGTGGTGTGGTGAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCt                                                                                                                                                                                                                        <  1:750775/301‑1 (MQ=255)                                                                                   ggTATCGTGATCGAGAAGTCGTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAACCCCCTTCGGCtt                                                                                                                                                                                                                 >  2:297988/1‑300 (MQ=255)                                                                                           gatcgagaagcagttgcgagtgggcCGTGCCGAACTGGACAGGTAGGGTTACCGCGTTGAAGTGAGGGCCCGGGTGAAGTAGCTGGAAGGGGGTGTGGTCAGGTGCATCGAATAAACGCGCCACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGGGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                                                                                                                                                                                                         <  1:520176/279‑1 (MQ=255)                                                                                                  aaGTCGTTCCAGTGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGCAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCCGCTGCCCCAACTGCATccc                                                                                                                                                                                                  >  2:357371/1‑300 (MQ=255)                                                                                                                         cGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATAccccc                                                                                                                                                                           >  1:430267/1‑300 (MQ=255)                                                                                                                                             acctcgttggatctgtGGCCCGGGGGAAGTCGCTGGAAGGTGGGATGGTCAGCTTAATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACCGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAAc                                                                                                                                                       <  2:424152/286‑1 (MQ=255)                                                                                                                                                   ttGAATCGCTTGCCCGGGTGAAGTCGCGGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACCCCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGcc                                                                                                                                                 <  1:318742/301‑1 (MQ=255)                                                                                                                                                      aaTCCCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGACCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTg                                                                                                                                              <  2:430089/301‑1 (MQ=255)                                                                                                                                                            tGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCCACTGCATCCGCCCCAGGTGGCTTTCGTACCACCCCCGCCCCGGTCCATGCAACGCTGACTTGCCCatc                                                                                                                                        >  2:177608/1‑299 (MQ=255)                                                                                                                                                                ccGGGTGAAGTCGCTTGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCACAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCa                                                                                                                                    <  1:298102/301‑1 (MQ=255)                                                                                                                                                                ccGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCCTCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGccccc                                                                                                                                    >  2:141238/1‑297 (MQ=255)                                                                                                                                                                       aaGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGTCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTc                                                                                                                             >  2:134520/1‑301 (MQ=255)                                                                                                                                                                           cGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTc                                                                                                                         >  1:151923/1‑301 (MQ=255)                                                                                                                                                                              tGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCCCTCCCACAGGCGCAGCACCGGTCTCGAACGCTGTGCCGTACCTGTGGTAGCGGTCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCGCCCCCAACTGCATCCGCCCCATGTGGCTTTCATACTCCTCCGGCCCCGGTCCATCCAACGCTGCCTTGCTCAGCTCCAGCAcccccatcagc                                                                                                                      >  1:22813/1‑292 (MQ=255)                                                                                                                                                                               ggAAGGTGGTGTGATCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCt                                                                                                                     <  2:151853/301‑1 (MQ=255)                                                                                                                                                                                gAAGGTGGGGGGGTCAGCGTCACCGAATAAACGCGGCACCGGCGTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGGGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCtt                                                                                                                    <  2:84949/301‑1 (MQ=255)                                                                                                                                                                                gAAGGTCGTGTGGGCAGCTGCATCTAAGAAACGCGGCACCTGCTTCGAGGGCCCGCCGGCTCCAACAGGTGCAGAAAAGGTCTAGAACGCCGTGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCtt                                                                                                                    <  1:660257/301‑1 (MQ=255)                                                                                                                                                                                         tcacctaGCTTCATCGACGAAATGCGACTCCTGCTTCGCGGCCACCGCCGCACACACAGCTGCAGTTCAGGTCTCGACCGGTGTGCCGTACCTGCGTGAGCGGGCGTGCCCGCGACTGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCCACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCCCCTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGct                                                                                                           <  1:379/295‑1 (MQ=255)                                                                                                                                                                                                             aaCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCACGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCACCACTGCACTGCCCAGCACCACCACCCCGCTCAGCTTGCACTTGCTCTTCACCCACTTCAGCcccc                                                                                       >  2:703746/1‑301 (MQ=255)                                                                                                                                                                                                                                gggCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGTGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGctcctc                                                                    <  1:357506/301‑1 (MQ=255)                                                                                                                                                                                                                                             cccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTcc                                                       >  1:666854/1‑301 (MQ=255)                                                                                                                                                                                                                                                        agcaCAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCCCCAGCTCCTCGCTCAGCTTGAACTCGCCCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCAGTGCCCaccgc                                            >  1:238209/1‑297 (MQ=255)                                                                                                                                                                                                                                                        agcaCAGGTCTCGAACGCGGTGACGTACCTGTCGGCGCGGGCGGGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCgg                                            <  2:246640/301‑1 (MQ=255)                                                                                                                                                                                                                                                             ccctacccgacgctcttccgatcttgtgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCGGATACt                                       <  2:552642/277‑1 (MQ=255)                                                                                                                                                                                                                                                                        gCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCGGATACTCCGGGAACAGc                            >  1:459405/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                    cTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCGGATACTCCGGGAACAGCCGCGCATGCtgt                >  1:738046/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                     tgtgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCGGATACtagatcggaagagcacacgtctgaa               >  1:552872/1‑277 (MQ=255)                                                                                                                                                                                                                                                                                       tgGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGACTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCCCTTCAACCAGTTCACCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCGCATACTCCGGGACCAGCCGAGCAtccggcgcc             >  2:75044/1‑293 (MQ=255)                                                                                                                                                                                                                                                                                                  gTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCGGATACTCCGGGAACAGCCGCGCATGCTGTGCCCTGATCGCCTc  >  1:514824/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                  gTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCCCCCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGAACCCGCTACCCGGCCGTCAGGTCCGTGACCACCGGATACTCCGGGAACAGCTCCGCTTGCGGGGCCCTGCTCGcccc  >  2:648736/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        CGATGACTTCCTGGCCAACGGCAAAGCCTCCCAAGCGCTGATCTCGATCATCAAGCAGGCAGGTGCCACCGTGGCGGGCCTGGGTATCGTGATCGAGAAGTCGTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCGCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGGCTTTCATACCCCTCCGGCCCCGGTGCATCCAACGCTGCCTTGCCCAGCTCCAGCACCTCGCTCAGCTTGAACTTGCTCTTCAACCAGTTCAGCGCCCGCAACAGATCCCGCTCCTCGGCCGTAAAGTCCGTGCCCATCGGATACTCCGGGAACAGCCGCGCATGCTGTGCCCTGATCGCCTC  >  AE015451/6013591‑6014180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑