| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | 23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 1,932,638 | 1 | . | C | 96.4% | 184.3 / ‑2.9 | 57 | intergenic (‑105/+45) | rluA/minE | 23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (28/26); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AGGATGATGCCGGAGGTTTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGTCAAAAAG > AE015451/1932340‑1932931 | gtgtgctcttccgatctttCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑cc < 1:1181817/285‑1 (MQ=255) atgatgCCGGAGGTTTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACACAGGCTATGCGTCCCCTGGCGGAGCGGGCTT‑G‑cccgc > 1:2267273/1‑298 (MQ=255) gCCGGAGGTTTCCCAGTCCCGCCGGTGCACGATCAGCTCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCGTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAgg < 2:472437/301‑1 (MQ=255) cGGAGGTTTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGTATCGGGCTT‑GCCC‑GCGAAGGGc > 2:835665/1‑301 (MQ=255) ttCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCTGGCAAGCCCGCGCCCACAAAGGATATGCGTCCCCTGTAGGGGCGGGCTT‑G‑cgcgatcggaagagcggcg > 2:1181369/1‑283 (MQ=255) cccAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGcccc < 1:2239722/300‑1 (MQ=255) tctcggtgaaGCCGGTTTTCTGCAGACGGG‑GACACAGGCATTACTTGTT‑TCACTCGGGACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTAGATCACCAGGTTGTCGGCGTATTCGAAGGGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTACTACAAAGGATATGCGTCCCCTGGAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCGCCAAATTGTCGACTACGCGGATCa < 1:61453/292‑1 (MQ=255) tatcgtggtggccggtagccTGCAGACGGGTGT‑TCAGGCAGTCTGTGTTGTC‑CTCGGCACGGCCAGGTACCGCCAGCAGCAGGGTCCGTTTGTTGATCACCAGGATGGGCGCGTCTTCAAAGAGGATCTGGACATTCGACAGCAGCATGGCAGGTCTCAAGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa < 1:463068/283‑1 (MQ=255) aTCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCATGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAACATAGCAGTAAACGCTGGGGCCGCTTTGCGGCTCTTCGCGGGCACGCCCGCTCCTACAAAGGCTCCGCGCCCCCTGTAGGAGCGGGCTTCC‑CC‑GCGAAGGGCCGCCCTGAAGCCCCACATTGTCGACTACGCGGATccc > 2:154853/1‑299 (MQ=255) cGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCg > 1:1063017/1‑301 (MQ=255) ggggTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAgatcggaagagcacacgtctgaactccagtc > 1:557313/1‑271 (MQ=255) ccGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCGCGCTCCTACCAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCACGGCAGCCCCAAATTGTCGACGACGCGGATCAGCCATCCgtcc > 2:1738015/1‑298 (MQ=255) gTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAACCCCGCTCCTACCAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCCGGCCAGCCCCACATTGTGGACTACGCGGATcgggcatccgcgacc > 2:263845/1‑287 (MQ=255) cTGCAGCCGGGTGT‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 2:1062627/301‑1 (MQ=255) aGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCCCCTACAAAGGATATGCGTCCCCCGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCCGCAAGGCAGCCCCCAATTGGCGACTACGCGGATCAGCGATCCGGCAAGGCGATgctcc > 1:1260698/1‑297 (MQ=255) tttgtggTC‑ATCGGCACGGCCAGTTACGGAGAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTCCGAAGAGCATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTg < 2:1331639/300‑1 (MQ=255) gttgttgTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGAATCTGGACATTGGAGAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAGCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGAAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGAAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTg < 1:154897/300‑1 (MQ=255) tgttgtC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCACCGAGCCGGCAGGGTGATGGTCAGCCTCAGAATCGAGCAGCTGcc > 2:1739958/1‑301 (MQ=255) tgtC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg > 2:1802936/1‑301 (MQ=255) gcGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTGTTCGAAGAGGATCTGGACATTCGACAGCGGCATGGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt < 2:1514250/300‑1 (MQ=255) aGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGAGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata < 2:475007/301‑1 (MQ=255) gtagcggcagtggcagGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTGGAAGAGGATCGGGACATTCGACAGCGGCATTGCAGGGCTCACGAAAAGAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGAGATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg < 1:263946/289‑1 (MQ=255) ggcagcagcagGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcat < 1:835952/299‑1 (MQ=255) gcagcagGGTGGGTTTGTTGATCACCAGGATGGAGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 2:1260227/301‑1 (MQ=255) ggggcgggttgttggacACCAGGATGGCGGGGTCTTCGAAGAGGATCTGGACATTCGACGGGGGCATGGCAGGTCTCACGAAAATAGCAGTAAGCGCTGGGGCCGTTTTGCGGCCCTTCGCAGGCAAGCCCGCTCCTACAAAGGATAGGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc < 1:662198/285‑1 (MQ=255) cGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATg < 1:1803662/301‑1 (MQ=255) ttgttgATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGTGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTCGCCGATGTTca > 1:622226/1‑301 (MQ=255) aGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTCTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCCCCAGAATCCAGCAACTGCTCTGGTTTTCCCGCTCGATCTGCACatcatctctgtcgatggtcaacttttttctgt > 2:138357/1‑275 (MQ=255) gggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTc < 1:1738714/300‑1 (MQ=255) cggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGCGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGTCTGCAAGGCATCCCCAAATTGTCGACTACTCGGATCAGCGATCCGGCACGGGGATGGTCAGCTCCATAATCGAGCAGCCGCCCTGGTGCTCCAGCTCCATATGCAAATCATGGCTGCCGATGTTCACATGTTTGCCGATCACTTc > 2:2100655/1‑301 (MQ=255) ggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCCAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGGGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACCTTTTTGCGGATCCCTTcc > 1:1554702/1‑301 (MQ=255) ctggagttcagacgtgtgctcttccgatctcGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCcagc < 1:2325629/271‑1 (MQ=255) cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCCGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCCCGATATGCACATCATCCTTGCCTATGTTCACATATTTGCGGATCACTTCCAGCAGttc > 1:1491542/1‑301 (MQ=255) gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCTGCTCGATATGCACATCATCGTTGCCGATGTTCCCATATTTGCGGATCACTTCCAGCAGttct > 1:743067/1‑301 (MQ=255) gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGttctttt > 2:1197118/1‑300 (MQ=255) agGACCTGGCCATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGttctttct < 1:1740657/301‑1 (MQ=255) cagacgtgtgcgcttccgatctGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAgc < 1:1750786/280‑1 (MQ=255) cTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGACGTTCACATATTTGCGGATCACTTCCAGCCGTTCTTTCTGCacc > 1:2078921/1‑299 (MQ=255) cGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTTCCGATGTTCACATATTTGCGGATCACTTCcagcagatcggaagagcgtcgtgtagggaaagag > 2:2324695/1‑273 (MQ=255) gCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCAATTCCAGCAGTTCTTTCTGCaccagatcggaagatcgatgggtag > 2:1750087/1‑277 (MQ=255) ctcACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGtt < 1:1197566/301‑1 (MQ=255) aTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGTTAGTCGGGTTCGCTGCGTTTGCCgcgcc > 2:632942/1‑300 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCGCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTG‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCAc < 1:138400/301‑1 (MQ=255) cGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAACGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATCCACATCATCGTTGCCGATGTTCCCATATTTGCGGATCACTTCCACCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCTCGCTCGCTCGCCACgatgttgtgt > 2:2254411/1‑295 (MQ=255) cGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACCTATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCAGCATGCTCTGTAGACGcc > 1:757401/1‑300 (MQ=255) ccTTAGCGGGCGAGCCCGCTCCTACAAAGGATATGCGGCCCCTGTAGGAGCTGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAATTTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGCTGATCTGTAGACGCTCttt < 1:2101495/301‑1 (MQ=255) acgtgtgctcttccgatctcTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑Cg < 1:901799/282‑1 (MQ=255) ctttccctacacgacgctcttccgatcTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCt < 2:276173/275‑1 (MQ=255) ccggtacgaaaaaggatattgttCCCCTGTAGGAGCGGGCTT‑GTCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCt < 2:757131/279‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCCGGTAGTCCGGTTCGCTGCGTTTGCCGCGCTCGTTCGCCACGATCATCTGTAGCCGCTCttccgagatcggaagagcgtcgtg > 2:901489/1‑279 (MQ=255) aGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCACGCAGTTCTTTCTGCAGCGGGGGCAGGTAGTCCGTTTCCCTGCGTTGGCCGCGCTCGTGCGCCAGGATGGTCTGTAGCCGCTCTTT‑CGCTACCGAccccgtggttggtttctt > 2:1896106/1‑284 (MQ=255) ttggCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCTGGTCTGTTtctgtctg < 2:742797/298‑1 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGC‑CTTTCCGCTACCGCCGCGCTGGTCTGTTTCTGTCTGc > 2:1674362/1‑301 (MQ=255) ggtcggcgggaGGAGCGGACGT‑G‑CCAGCGCAGGGCTGCAAGGCAGCCCCAAACTGTCGACTACGCGGATGAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGGTGCCGATGTTCACATATTTGCGGATCGCTTCCAGCAGTGCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGCTCTGTAGACGCTCTTT‑CGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCAc < 1:398217/291‑1 (MQ=255) gTCCCCTGTAGGGGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACg < 2:1490953/301‑1 (MQ=255) cccTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGaaa < 1:633177/301‑1 (MQ=255) cTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCGCTTT‑CGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAaaga > 2:305546/1‑301 (MQ=255) gTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCtagatcggaagagcacacgtctgaactcc > 1:276274/1‑273 (MQ=255) aGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTTCGCCACGATGATCTTTAGACGCTCTTT‑CGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGTc > 1:2219120/1‑301 (MQ=255) gggCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGTCAAAAAg > 1:127713/1‑301 (MQ=255) | AGGATGATGCCGGAGGTTTCCCAGTCCAGACGGTGCACGATCAGCGCATCGGGGTAGCCGTTTTCCTGCAGACGGGTGA‑TCAGGCAGTCCTTGTTGTC‑CTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCCACGATGATCTGTAGACGCTCTTT‑CGCTACCGACGCGCTGGTCTGTTTCTGTCTGCCACGAAAGAAGTCAAAAAG > AE015451/1932340‑1932931 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |