Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451	4,586,057	+C	intergenic (+140/+75)	PP_4061 → / ← PP_4063	conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,586,056	1	.	C	97.1%	229.2 / ‑1.6	68	intergenic (+139/+76)	PP_4061/PP_4063	conserved protein of unknown function/putative Long‑chain‑fatty‑acid‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (31/35);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

ACATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TCTGC‑G‑G‑T‑AAACCCGCTCCTACCAGG‑G‑A‑CGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGCGACGATCTCTTCGCCATAG  >  AE015451/4585760‑4586351                                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                         aCATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑A‑ccg                                                                                                                                                                                                                                                                                                       <  1:680146/301‑3 (MQ=255)   cttccgatctcACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑A‑ccgtg                                                                                                                                                                                                                                                                                                     <  1:1789760/291‑5 (MQ=255)            agacgtgtggtcttccgatctaaTATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCACGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTg                                                                                                                                                                                                                                                                                             <  1:2083936/280‑1 (MQ=255)             cACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCCC‑TTCGCGG‑G‑T‑AAACCCGCTCCCACCAGG‑G‑A‑ccgtgcgatcggaag                                                                                                                                                                                                                                                                                           >  2:1789060/1‑287 (MQ=255)              aCCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCGTAAGGGTAGACCACGGCACCGGCGTGAACAGTCGATCTGCGCTGCTCCCTCGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGGGAAGAGCTTGA‑ATTGATCGGGCGGGACTCTTCGGG‑G‑G‑T‑AAACCCGCTCCGACCAGG‑GAA‑CGTGCCGGctgtcgg                                                                                                                                                                                                                                                                                           >  2:1101491/1‑295 (MQ=255)                cGGAGACACCCGATCCCAAAATCGACGCCCCGGGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATg                                                                                                                                                                                                                                                                                         <  1:799390/301‑1 (MQ=255)                    aacacCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATgcggc                                                                                                                                                                                                                                                                                     <  1:388978/300‑1 (MQ=255)                          cGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑ttcc                                                                                                                                                                                                                                                                              >  1:1463790/1‑298 (MQ=255)                              cccAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTg                                                                                                                                                                                                                                                                          <  1:473118/301‑1 (MQ=255)                               ccAATATCGACGACCCGAGCTTGCCGCCCCCCGGACCGGAAGAAGACCCGGACGAGCGGCCGATCAAGCCGACCGGGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTgg                                                                                                                                                                                                                                                                         <  2:862240/301‑1 (MQ=255)                                 aaTATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACGCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGACCTGCGCTTCCCCAGTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGCAGATCTTGC‑CTTGATCGGGCCTGCCTC‑TTCGC‑G‑GCT‑AAACGCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTgcgatcggaagtgacacgtgtg                                                                                                                                                                                                                                                                        >  2:2083096/1‑280 (MQ=255)                                  ctagagaagcgccgtgcctGCCGCCACCCGGACCGGAAGAGGACCCGGAGGGGCTGCCGCTCAAGCCGACCGTGCCGCCGGCAGTGGGCGAGCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGGTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAg                                                                                                                                                                                                                                                                      <  1:1101916/283‑1 (MQ=255)                                      ggagttgtgtcctgccgatctaCCCGTACCGGAAGAAGGCCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCCCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGc                                                                                                                                                                                                                                                                  <  1:1481528/280‑1 (MQ=255)                                        ccgacCCGGGCTTGCCGCCACCCGTCCCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCgg                                                                                                                                                                                                                                                                <  1:61697/300‑1 (MQ=255)                                        acgacCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCgg                                                                                                                                                                                                                                                                <  1:1301972/301‑1 (MQ=255)                                        aacccgacgctctcccgacatccGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCgg                                                                                                                                                                                                                                                                <  2:1259432/280‑1 (MQ=255)                                          gccccGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATGAAGACGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGt                                                                                                                                                                                                                                                              <  1:65644/299‑1 (MQ=255)                                           ccccGAGCTTGCCTCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGATTGACAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGACCCTGGTAGGAGCGGGtt                                                                                                                                                                                                                                                             <  1:551774/300‑1 (MQ=255)                                                  cTTGCCGCCACCCGTACCGGAAGAAGGCCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGAAAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCcgc                                                                                                                                                                                                                                                      <  2:2079767/301‑1 (MQ=255)                                                   gtgtgctcttccgatctggAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCcgcg                                                                                                                                                                                                                                                     <  1:471936/284‑1 (MQ=255)                                                           aCCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTTCCGGCCTTCAATGCGGCGC‑TGCCCCTCGTAGGAGCGGGTTTACCCGCGCAGAggcc                                                                                                                                                                                                                                             >  1:1761872/1‑301 (MQ=255)                                                           aCCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCCCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAGGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCACCTACCAGG‑T‑ACCGTGCCGGCCTTGAATGCGG‑GCTTGCCCCTGGTCGGAGcagatcgtacgaccgtcgcgtt                                                                                                                                                                                                                                             >  2:1480939/1‑280 (MQ=255)                                                             ccGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCggagatcggaagagcacacgtct                                                                                                                                                                                                                                           >  1:1259903/1‑280 (MQ=255)                                                                   cGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCgcgc                                                                                                                                                                                                                                     >  1:621371/1‑301 (MQ=255)                                                                    ggAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGTAGCGGGTTTACCCGCGagatcggaagggcgtcg                                                                                                                                                                                                                                    >  2:471754/1‑285 (MQ=255)                                                                          acgtgtgctcttccgatctaTCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGcaca                                                                                                                                                                                                                              <  1:1773673/282‑1 (MQ=255)                                                                             ccgggACGAGCTGCCGATCAAGCCGACCGGGCGGCCGACAGGGGGCGCCCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACGGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTGGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCa                                                                                                                                                                                                                           <  2:2058966/298‑1 (MQ=255)                                                                               gggaagagaggCCGATCAAGCCGACCGTGCCGCCGACGGGGGGCGACCCGGCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTAATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAAc                                                                                                                                                                                                                         <  2:1345340/291‑1 (MQ=255)                                                                                ggACGAGCTGCCGATCAAGCCGACCGTGCCGACGACAGTGGCCGACCCGCCGAGCCAGGAGCCACCGTTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAAcc                                                                                                                                                                                                                        <  2:174893/301‑1 (MQ=255)                                                                                   ggagccgcggctCAAGCGGACCGTGCCGCCGACGGTGGGCGAGCCGCCGCGCCAGGAGCCACCGGTTAAGGCTTAAGGGTAGGCCACGGCACCGGCGTGATGAGCTGATCGGCGCGGCTACATTGTAAGGTTGAGGATGACCGGGTGCTTAGCAAGGTGAAGATCTTGC‑GTTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑T‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGc                                                                                                                                                                                                                     <  2:10947/290‑1 (MQ=255)                                                                                            gATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCGGGTCATACCAGG‑G‑ACCGTGCCGCGCTTGAATGCGGGGC‑TGACCCTGGGAGGAACGGGTTTACAGGCGAAAAGGCCGGCGGCGGCAgtaacataagcagagtatgcagg                                                                                                                                                                                                            >  2:62159/1‑279 (MQ=255)                                                                                             aTCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAAAAGCCGGCCGCTCCAGcaaaaataggggagaggcgcggg                                                                                                                                                                                                           >  2:1772974/1‑280 (MQ=255)                                                                                                    cGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCCCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACCTCAACCCGCAGATGCAGCCGCAAtcc                                                                                                                                                                                                    >  1:831818/1‑300 (MQ=255)                                                                                                      ccttccgatcggACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTCGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTcc                                                                                                                                                                                                  <  1:595889/290‑1 (MQ=255)                                                                                                              ccccgctgtcctcccggtcccgAGCCGGGAGCCCCCGGTGAAGGCTTAAGGGTAGCCCACGGCACCGGCGTGAACAGCTGATCTGGTCTGCTCATTTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACt                                                                                                                                                                                          <  2:890079/282‑1 (MQ=255)                                                                                                                 gACAGTGGGGGACCGGACTAGCCAGGAGGCACCTGTGAAGGCTTAAGGGTAGACCACCGCACCGGCGTGAACAGCTGACCTGTGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGCGAAGATCTTGC‑ATTGATCGAGCCGGCCTC‑TTCGCGG‑G‑T‑AGACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGAt                                                                                                                                                                                       <  1:2195540/301‑1 (MQ=255)                                                                                                                 gACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTCGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAAACCGCAGCTGCAGcacaaatccccagatcggaaga                                                                                                                                                                                       >  2:595676/1‑280 (MQ=255)                                                                                                                    aGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAAACCGCAGATGCAGCCGCAATCTCCGCCACACTGAtctc                                                                                                                                                                                    >  1:2259137/1‑301 (MQ=255)                                                                                                                            aCCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGTCTC‑TTCGC‑GCG‑T‑AAACCTGCTCCTACCAGG‑G‑ACCACGCCCGCCTACCATGCGGCCC‑AGCTCCTGGCAGTAGCGGGGTGACCCGCTAAGAGCCCGGTCGCGCCAGCAACCCAGCCCGCATATGCAAACGCAATCTCCGTCacacacctcttacacatcc                                                                                                                                                                            >  1:495275/1‑286 (MQ=255)                                                                                                                             cccGCCGAGACAGGAGGAACCGGTGAAGGCGTAAGGGTAGGCCACGGCACCGGCTTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGGGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCg                                                                                                                                                                           <  2:1463224/301‑1 (MQ=255)                                                                                                                                  cgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACtagatcggaagaacacaagtc                                                                                                                                                                      >  1:890389/1‑281 (MQ=255)                                                                                                                                  cgAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACACCTGCGGAACt                                                                                                                                                                      >  1:161399/1‑301 (MQ=255)                                                                                                                                        gggaggaaCCGGTAAAGGGTTAAGGGTAGACCACGGCACCGACGTGAACAGCTATTCTGCGCTGCTCCATTGAAGGGTTGAGGATGACCGGGTGCTAGGCAAGGTTAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCCC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCa                                                                                                                                                                <  2:831532/294‑1 (MQ=255)                                                                                                                                                ccGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGAGCTTGC‑ATTGATCGGGCCGGCCTCTTCGGG‑G‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑GGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCcg                                                                                                                                                        <  2:621137/301‑1 (MQ=255)                                                                                                                                                    tGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCccgtcc                                                                                                                                                    >  1:470616/1‑300 (MQ=255)                                                                                                                                                                   aGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGC‑G‑G‑G‑TAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTATCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCTCCTTGCCGGTCACGGTCATCGGGTACtc                                                                                                                                    >  1:606149/1‑301 (MQ=255)                                                                                                                                                                       tgctcttccgatctGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACtcgtc                                                                                                                                 <  1:1007610/288‑1 (MQ=255)                                                                                                                                                                       cACGGCCCCGGCGTGAACAGCTGTTCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGTGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AACCCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACtcgtt                                                                                                                                 <  2:2258218/301‑2 (MQ=255)                                                                                                                                                                                     gAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAAGCTCCGCCACACTGATCGCACGCATGCGGAACTTCTGCACCTTGCCTGTCACGGTCATCGGGTACtcggcagatcggaagagcg                                                                                                                   >  2:1007246/1‑285 (MQ=255)                                                                                                                                                                                      aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCCCGGGTATCGGGTACTCGTCGCCAAAACGtatata                                                                                                                  >  1:1124647/1‑301 (MQ=255)                                                                                                                                                                                       acacttGATCTGCGCTTCTCCATTGAAAGTTTGACCATGCCCGGGTGCTTGGCCCGGTGCAGATCTTGC‑ATTGATCGGGCCGTCCTC‑TTCGC‑GTG‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGTATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATAc                                                                                                                 <  1:62173/296‑1 (MQ=255)                                                                                                                                                                                            tGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCGGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCCATCTCCGCCACACTGATCTCTCGCATGCGGAACTTCTGCACCTTTCCGGTCCCGGTCATACGGTACTTGCCGATAAAAAGtattagtcgcgg                                                                                                            >  2:1543606/1‑292 (MQ=255)                                                                                                                                                                                              tcgtgcgcagtgcgatgaaaaGGTGGAGGATGAGCGGGTGCTTGGCAAGGTGAAGATATTGC‑ATGGATCGGGCCGGCCTC‑TGCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGACGGCCTGGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCa                                                                                                          <  2:1991534/283‑1 (MQ=255)                                                                                                                                                                                                                   ggtggaggtgacccgtgTGCTGGGCAAGGTGAAGATCTTGC‑ATAGATCGGGCCGGCCTC‑TTAGCGG‑G‑T‑CAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCGTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTg                                                                                     <  2:717051/285‑1 (MQ=255)                                                                                                                                                                                                                     gtGAGGAGGACCGGTTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGGTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGcc                                                                                   <  2:1761173/300‑1 (MQ=255)                                                                                                                                                                                                                             gACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCCCCGCCACACTGATCTCACGCATGCGGAACTTCTCCACCGTGCCGGCCACGGTCCTCGGGTCCTCTGCGACAAAACGTATATACCGCGGCCCCTCTCAGTGTGCGCTTCGTGCCTTGCAccc                                                                           >  2:2088354/1‑300 (MQ=255)                                                                                                                                                                                                                                      cTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCAACTTGCCGGTCACGGTCATCGTGTACTTGTCGACAAAACGTATCTACCGTCGCACCTTGAAGTGCGCCAAGCGTGCCTTGCACCAGCCCTGCac                                                                  >  1:1838534/1‑300 (MQ=255)                                                                                                                                                                                                                                          gCAAGGTGAAGTTC‑TGCAATTGTCCGGGCCGGCCTCTTCT‑C‑G‑G‑TCAAACCCGCTCCTACCAGGAG‑C‑CGTGCGGGCCTTGATTGCGGCGC‑TGCCCCTGGTAGGGGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGctcc                                                              <  1:1544209/301‑1 (MQ=255)                                                                                                                                                                                                                                                   aGATCTTGC‑ATTGATCGGGCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGAccctg                                                     >  1:2246145/1‑298 (MQ=255)                                                                                                                                                                                                                                                                    gggCCGGCCTC‑TTCGCGG‑G‑T‑AAACCCGCTCCTGCCAGG‑G‑ACCGTGCCGGCCTGGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGt                                     <  2:470434/301‑1 (MQ=255)                                                                                                                                                                                                                                                                           cccctttcgcgg‑g‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCt                               <  1:1768401/290‑1 (MQ=255)                                                                                                                                                                                                                                                                            cctcttcgcgg‑g‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACCAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGTGGCAGCtt                              >  2:2191077/11‑301 (MQ=255)                                                                                                                                                                                                                                                                              tcttcgcgg‑g‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTgg                            >  1:1133875/9‑300 (MQ=255)                                                                                                                                                                                                                                                                               cttcgcgg‑g‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTTCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCTGCACCTTGACGTGTGCGATGCGTCCCTTGCACCAGCCCTGCAGCTCCCCTACCGTGTCCCTTTGCCCCCGGTTCCGCTTGAt                           >  2:1815900/8‑301 (MQ=255)                                                                                                                                                                                                                                                                                     gg‑g‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTAGTAGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTTCGATGCGTGACTTGCACCAGCCCCGCAGCTTCTCGACCGTCGCGCTATGCACCGGGGTCAGCTTGATCCAGGc                     >  1:1672806/2‑301 (MQ=255)                                                                                                                                                                                                                                                                                        g‑T‑AAACCCGCTCCTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCCCGCCTGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAGACGTCTATACCGCTGCACCTTGAAGTGTGAGATGCGTGCCTTGCACCCTGCCTGCAGCTCCTCGACCGGGGCTCTGTTCCCCGGGCGCAGCTTGCTCCAGGcgc                   >  1:36603/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            aaaCCCGCTCCTACCAGG‑G‑ACCGTGCCTGCCTTGAATGCGGCCC‑GGCCCCTGGTATGAGCGGGTTTACCCCCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGcgacg                 <  2:1124222/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ccGCTCCTACCAGG‑G‑ACCGTGACGGCCCTGAATGCGGAGC‑TGCCCCTGGTAGGAACGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGCGACGAtct             <  1:2089194/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     ccTACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGCGACGATCTCTTcc        >  1:1418038/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       tACCAGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTCCGATGCGTGCCTTGCACCAGCCCTGCAGCCCCTCGACCGTTGCGCTGTTCCCCGGGGTCAGCTTGGTCCCTGCCGCGATCTCTTcccc      >  2:1737822/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                           aGG‑G‑ACCGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGCGACGATCTCTTCGCCATAg  >  1:1145409/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                         ACATTCCCATTCCACCGGAGACACCCGATCCCAATATCGACGACCCGAGCTTGCCGCCACCCGTACCGGAAGAAGACCCGGACGAGCTGCCGATCAAGCCGACCGTGCCGCCGACAGTGGGCGACCCGCCGAGCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGC‑ATTGATCGGGCCGGCCTC‑TCTGC‑G‑G‑T‑AAACCCGCTCCTACCAGG‑G‑A‑CGTGCCGGCCTTGAATGCGGCGC‑TGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTGATCTCACGCATGCGGAACTTCTGCACCTTGCCGGTCACGGTCATCGGGTACTCGTCGACAAAACGTATATACCGCGGCACCTTGAAGTGTGCGATGCGTGCCTTGCACCAGCCCTGCAGCTCCTCGACCGTGGCGCTGTGCCCCGGGTGCAGCTTGATCCAGGCGACGATCTCTTCGCCATAG  >  AE015451/4585760‑4586351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑