Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	AE015451	4,980,585	+GGC	intergenic (‑24/+44)	PP_4387 ← / ← flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4980585	1 (0.010)	59 (0.850) +GGC	53/594	0.2	98.4%	intergenic (‑24/+44)	PP_4387/flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE
	?	AE015451	4980586 =	1 (0.010)					intergenic (‑25/+43)	PP_4387/flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE

CCGGTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGT  >  AE015451/4980286‑4980876                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                       ccGGTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCGAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGg                                                                                                                                                                                                                                                                                                       <  1:240881‑M1/301‑2 (MQ=255) ccGGTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGg                                                                                                                                                                                                                                                                                                       <  3:180614‑M1/301‑2 (MQ=255)  cGGTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGTGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGgg                                                                                                                                                                                                                                                                                                      >  1:563873‑M1/1‑299 (MQ=255)          ggggCTTTCAGCGCGGCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTGCACATTCTTGGggccgctttg                                                                                                                                                                                                                                                                                              >  4:19736‑M1/1‑291 (MQ=255)            ggcttgaagcgcggcAAGGGGCAATACCCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcg                                                                                                                                                                                                                                                                                            <  2:19736‑M1/294‑13 (MQ=255)            cacacgaccgtcttccgatcccaataaccgtcagtttgtcggcGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcg                                                                                                                                                                                                                                                                                            <  2:627415‑M1/280‑13 (MQ=255)                        ggCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattaca                                                                                                                                                                                                                                                                                <  1:79506‑M1/301‑25 (MQ=255)                           gagGGGAAATAAGCGGCAGTTTGGCGGCTGCTGTTTGCGGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGGGGGGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGACGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaac                                                                                                                                                                                                                                                                             <  2:629213‑M1/300‑28 (MQ=255)                                 cAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcgagatcggaagagcacccgtcg                                                                                                                                                                                                                                                                       >  4:627618‑M1/1‑268 (MQ=255)                                    tAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagcc                                                                                                                                                                                                                                                                    >  1:598234‑M1/1‑265 (MQ=255)                                      aCCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaa                                                                                                                                                                                                                                                                  >  4:96242‑M1/1‑263 (MQ=255)                                                       ggATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGgtccgcttttcggccccaattacaaacacccacgccaataccatcatcaggtcct                                                                                                                                                                                                                                                 >  4:768569‑M1/1‑246 (MQ=255)                                                               gccgccTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTTAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatga                                                                                                                                                                                                                                         >  1:357952‑M1/1‑238 (MQ=255)                                                                  gccTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatgg                                                                                                                                                                                                                                      >  1:108813‑M1/1‑235 (MQ=255)                                                                  gccTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattaaaaacaccaaagccaatacaatcatcaggtcatctgaatgatgg                                                                                                                                                                                                                                      >  1:37316‑M1/1‑235 (MQ=255)                                                                     ggggcaaagggtctgcgcaatgggctcggcgggcgGCGTTGTTGTAGGGGCCAGAGGGGCGCAGCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtct                                                                                                                                                                                                                                   <  2:96221‑M1/284‑70 (MQ=255)                                                                     ggcGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtct                                                                                                                                                                                                                                   <  3:108816‑M1/300‑70 (MQ=255)                                                                     caccgcgatgactggctccatggtgtcgGCAGGCGTCTTCGTTGTAGGGGCCATACTGCCGACCCCACTCTTTGCCCGGTTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATCGAACGGGGCGGGGGCCGCGGGCGCAGGCCGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCCCATTCTTGGGGCCGCTTTGCGGCCCCAATTCCAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCt                                                                                                                                                                                                                                   <  4:531678/275‑1 (MQ=255)                                                                         gCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatactatcatcaggtcatctgaatgatggtctgcat                                                                                                                                                                                                                               <  3:598236‑M1/301‑74 (MQ=255)                                                                          gaagGGGTTGCCCAATGGGCGCGGCAGGCGGCGGTGTTGTAGGGGCCAGCGTGGCGCACCCAGCCTTTGCCCGGGGTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCAGTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatg                                                                                                                                                                                                                              <  2:768289‑M1/300‑75 (MQ=255)                                                                                        aTGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagt                                                                                                                                                                                                                >  3:182019‑M1/1‑213 (MQ=255)                                                                                        aTGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagg                                                                                                                                                                                                                >  1:557814‑M1/1‑213 (MQ=255)                                                                                                  cAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtt                                                                                                                                                                                                      >  3:53397‑M1/1‑203 (MQ=255)                                                                                                      cggcgTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcagcacatctgaatgctggtctgcatgatggttctttacgtgggactgggtttgg                                                                                                                                                                                                  >  2:64482‑M1/1‑199 (MQ=255)                                                                                                          tctgttgtagGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcatt                                                                                                                                                                                              <  4:78095‑M1/299‑107 (MQ=255)                                                                                                             gttgtAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatgggtctttcaggggaactggttttggacttaaa                                                                                                                                                                                           >  2:78076‑M1/1‑192 (MQ=255)                                                                                                                  aGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGTACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggg                                                                                                                                                                                      >  1:446499‑M1/1‑187 (MQ=255)                                                                                                                      gCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAAAAAAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagtt                                                                                                                                                                                  <  1:53396‑M1/301‑119 (MQ=255)                                                                                                                         agagTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattccaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggccttggcctggtagttccc                                                                                                                                                                               >  4:52985‑M1/1‑180 (MQ=255)                                                                                                                                  cACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtggttgctctgcgcctt                                                                                                                                                                      <  3:37317‑M1/301‑131 (MQ=255)                                                                                                                                           tctcgtccgatcgtgggccgaacacagGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtt                                                                                                                                                             <  4:575919‑M1/289‑140 (MQ=255)                                                                                                                                                 ggggtTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccag                                                                                                                                                       <  3:357953‑M1/300‑146 (MQ=255)                                                                                                                                                  gggtgtggggccgaacaCAGGTAGCGGCCGGTGACAAGGCTTGGTCATTGACAGAACGGGGCGGGGGAAGCTGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATCCGCGTGAACCTTCACATTCTTGGtgccgctttgcggccccaattacaaacaccaaagccaatactatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgagcaggtttaccagc                                                                                                                                                      <  2:52968‑M1/294‑147 (MQ=255)                                                                                                                                                     ttGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcg                                                                                                                                                   >  3:605257‑M1/1‑152 (MQ=255)                                                                                                                                                        tgGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcaggtgaaatggtcttggcattggcctggtagttgctctgccctttgatcaggttagatcggaagagg                                                                                                                                                >  2:575734‑M1/1‑149 (MQ=255)                                                                                                                                                                   aagGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgac                                                                                                                                     <  3:557816‑M1/300‑164 (MQ=255)                                                                                                                                                                              ggtggaccaGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcct                                                                                                                          <  1:182021‑M1/297‑175 (MQ=255)                                                                                                                                                                              cGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgctcaggtttaccagctagccggtcaggtcgacgttggagtccc                                                                                                                          >  1:195577‑M1/1‑127 (MQ=255)                                                                                                                                                                              cGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagagatcggaagagcacacgtctgaactcaa                                                                                                                          >  4:765765‑M1/1‑127 (MQ=255)                                                                                                                                                                                  gACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccag                                                                                                                      >  1:673‑M1/1‑123 (MQ=255)                                                                                                                                                                                  gACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccag                                                                                                                      <  3:195575‑M1/301‑179 (MQ=255)                                                                                                                                                                                                  tGATAGTACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatc                                                                                                      <  3:446501‑M1/301‑195 (MQ=255)                                                                                                                                                                                                      aGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggc                                                                                                  <  1:605255‑M1/301‑199 (MQ=255)                                                                                                                                                                                                             gacgtgtgctcttccgatctgcagaaccagcaccagcaaggggaACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggt                                                                                           <  4:252575‑M1/281‑206 (MQ=255)                                                                                                                                                                                                                         gggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccgg                                                                               >  4:697030‑M1/1‑84 (MQ=255)                                                                                                                                                                                                                         gggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccgg                                                                               <  2:696800‑M1/301‑218 (MQ=255)                                                                                                                                                                                                                                 gcagAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtagatcggaagcgcgtcgggg                                                                       >  2:252517‑M1/1‑76 (MQ=255)                                                                                                                                                                                                                                   agAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggccccaccggaaatctggccgagggtaccggtgtccggagcgccggtc                                                                     >  2:771328‑M1/1‑74 (MQ=255)                                                                                                                                                                                                                                    aaacCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatca                                                                    <  3:673‑M1/300‑229 (MQ=255)                                                                                                                                                                                                                                     cagacgtgtgctcttccgatctaaggccagtaacgacagccgcttcATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcaacaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcac                                                                   <  3:115790‑M1/279‑230 (MQ=255)                                                                                                                                                                                                                                               agcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgccc                                                         <  3:563875‑M1/301‑240 (MQ=255)                                                                                                                                                                                                                                                        aaCAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgccggaggcatag                                                >  1:147865‑M1/1‑53 (MQ=255)                                                                                                                                                                                                                                                           aaGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcacagagcggaagagcgtcgtgtag                                             >  1:115788‑M1/1‑50 (MQ=255)                                                                                                                                                                                                                                                                  gTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgcggccgaggactctttcc                                      >  1:553392‑M1/1‑43 (MQ=255)                                                                                                                                                                                                                                                                                ttCATTCGCGTGAACCTTCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccgcagcgccgatccccgtcacgcccgaggcataggactctttccatttggtaccgcca                        >  4:687819‑M1/1‑29 (MQ=255)                                                                                                                                                                                                                                                                                  cagtcgggtgaacctTCCCATTCTTGGggccgcgttgcggccccaattaccaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgaggcataggactctttccagttggtaccgccaat                      <  2:687589‑M1/294‑275 (MQ=255)                                                                                                                                                                                                                                                                                            aaCCTTAACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgaggcataggactctttccagttggtaccgccaatcggcgtcagg            <  3:470018‑M1/301‑285 (MQ=255)                                                                                                                                                                                                                                                                                                ttCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgaggcataggactctttccagttggtaccgccaatcggcgtcaggcccc        >  1:228973‑M1/1‑13 (MQ=255)                                                                                                                                                                                                                                                                                                  cacaTTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgaggcataggactctttccagttggtaccgccaatcggcgtcaggccctgc      >  3:462924‑M1/1‑11 (MQ=255)                                                                                                                                                                                                                                                                                                   acaTTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgaggcataggactctttccagttggtaccgccaatcggcgtcaggccctgcc     >  1:288215‑M1/1‑10 (MQ=255)                                                                                                                                                                                                                                                                                                      ttCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgaggcataggactctttccagttggtcccgccaatcggcgtcaggcccttcaggt  >  2:116623‑M1/1‑7 (MQ=255)                                                                                                                                                                                                                                                                                                      ttCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgaggcataggactctttccagttggtaccgccaatcggcgtcaggccctgcaggt  <  3:288214‑M1/301‑295 (MQ=255)                                                                                                                                                                                                                                                                                                      ttCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctcgccggtcaggtcgacgttggagtcctccagggcaccaccggaaatctggccgagggtaccggtgtccggagcgccgatcaccggcacgcccgaggcataggactctttccagttggtaccgccaatcggcgtcaggccctgcaggt  <  4:771608‑M1/301‑295 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                       CCGGTTTACGGGGCTTTCAGCGCGGCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGT  >  AE015451/4980286‑4980876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.