Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	AE015451	4,980,585	+GGC	intergenic (‑24/+44)	PP_4387 ← / ← flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4980585	1 (0.010)	59 (0.850) +GGC	53/594	0.2	98.4%	intergenic (‑24/+44)	PP_4387/flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE
	?	AE015451	4980586 =	1 (0.010)					intergenic (‑25/+43)	PP_4387/flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE

GGGGCTTTCAGCGCGGCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGTTGGCGAAGTT  >  AE015451/4980295‑4980886                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                             ggggctttcagcgcggcaaggggcaataaccgtcagtttgtcggcggatgtttgccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaacctgcacattcttggggcCGCTTTg                                                                                                                                                                                                                                                                                                        >  4:19736‑M2/295‑301 (MQ=255)   ggcttgaagcgcggcaaggggcaatacccgtcagtttgtcggcggatgtttgccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCg                                                                                                                                                                                                                                                                                                      <  2:19736‑M2/9‑1 (MQ=255)   cacacgaccgtcttccgatcccaataaccgtcagtttgtcggcggatgtttgccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCg                                                                                                                                                                                                                                                                                                      <  2:627415‑M2/9‑1 (MQ=255)               ggcaaggggcaataaccgtcagtttgtcggcggatgtttgccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACa                                                                                                                                                                                                                                                                                          <  1:79506‑M2/21‑1 (MQ=255)                  gaggggaaataagcggcagtttggcggctgctgtttgcggcctgcggcaagggcttgctcaatgggcgcggcagggggggttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggacgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAac                                                                                                                                                                                                                                                                                       <  2:629213‑M2/24‑1 (MQ=255)                        caataaccgtcagtttgtcggcggatgtttgccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggccgctttgcgagatcggaagagcacccgtcg                                                                                                                                                                                                                                                                                 >  4:627618‑M2/272‑280 (MQ=255)                           taaccgtcagtttgtcggcggatgtttgccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGcc                                                                                                                                                                                                                                                                              >  1:598234‑M2/269‑301 (MQ=255)                             accgtcagtttgtcggcggatgtttgccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCaa                                                                                                                                                                                                                                                                            >  4:96242‑M2/267‑301 (MQ=255)                                              ggatgtttgccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttgggtcCGCTTTTCGGCCCCAATTACAAACACCCACGCCAATACCATCATCAGgtcct                                                                                                                                                                                                                                                           >  4:768569‑M2/250‑299 (MQ=255)                                                      gccgcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgttaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAatga                                                                                                                                                                                                                                                   >  1:357952‑M2/242‑301 (MQ=255)                                                         gcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATgg                                                                                                                                                                                                                                                >  1:108813‑M2/239‑301 (MQ=255)                                                         gcctgcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTAAAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATgg                                                                                                                                                                                                                                                >  1:37316‑M2/239‑301 (MQ=255)                                                            ggggcaaagggtctgcgcaatgggctcggcgggcggcgttgttgtaggggccagaggggcgcagccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCt                                                                                                                                                                                                                                             <  2:96221‑M2/66‑1 (MQ=255)                                                            ggcggcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCt                                                                                                                                                                                                                                             <  3:108816‑M2/66‑1 (MQ=255)                                                            caccgcgatgactggctccatggtgtcgGCAGGCGTCTTCGTTGTAGGGGCCATACTGCCGACCCCACTCTTTGCCCGGTTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATCGAACGGGGCGGGGGCCGCGGGCGCAGGCCGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCCCATTCTTGGGGCCGCTTTGCGGCCCCAATTCCAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCt                                                                                                                                                                                                                                             <  4:531678/275‑1 (MQ=255)                                                                gcaagggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACTATCATCAGGTCATCTGAATGATGGTCTGCat                                                                                                                                                                                                                                         <  3:598236‑M2/70‑1 (MQ=255)                                                                 gaaggggttgcccaatgggcgcggcaggcggcggtgttgtaggggccagcgtggcgcacccagcctttgcccggggtgtgggccgaacacaggtagcggccggtgaccaggctttgccagtgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCatg                                                                                                                                                                                                                                        <  2:768289‑M2/71‑1 (MQ=255)                                                                               atgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCagg                                                                                                                                                                                                                          >  1:557814‑M2/217‑300 (MQ=255)                                                                               atgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGt                                                                                                                                                                                                                          >  3:182019‑M2/217‑301 (MQ=255)                                                                                         caggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGgtt                                                                                                                                                                                                                >  3:53397‑M2/207‑300 (MQ=255)                                                                                             cggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGCACATCTGAATGCtggtctgcatgatggttctttacgtgggactgggtttgg                                                                                                                                                                                                            >  2:64482‑M2/203‑283 (MQ=255)                                                                                                 tctgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCAtt                                                                                                                                                                                                        <  4:78095‑M2/103‑1 (MQ=255)                                                                                                    gttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGGTCTTTCAGGGGAACtggttttggacttaaa                                                                                                                                                                                                     >  2:78076‑M2/196‑294 (MQ=255)                                                                                                         aggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagtacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCtggg                                                                                                                                                                                                >  1:446499‑M2/191‑300 (MQ=255)                                                                                                             gccagagtggcgcacccagcctttgcccgggttgtgggccgaaaaaaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGtt                                                                                                                                                                                            <  1:53396‑M2/115‑1 (MQ=255)                                                                                                                agagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTCCAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCCTTGGCCTGGtagttccc                                                                                                                                                                                         >  4:52985‑M2/184‑298 (MQ=255)                                                                                                                         cacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTGGTTGCTCTGCGCCtt                                                                                                                                                                                <  3:37317‑M2/127‑1 (MQ=255)                                                                                                                                  tctcgtccgatcgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGtt                                                                                                                                                                       <  4:575919‑M2/136‑1 (MQ=255)                                                                                                                                        ggggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAg                                                                                                                                                                 <  3:357953‑M2/142‑1 (MQ=255)                                                                                                                                         gggtgtggggccgaacacaggtagcggccggtgacaaggcttggtcattgacagaacggggcgggggaagctggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcatccgcgtgaaccttcacattcttggtgcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACTATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGAGCAGGTTTACCAGc                                                                                                                                                                <  2:52968‑M2/143‑1 (MQ=255)                                                                                                                                            ttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCg                                                                                                                                                             >  3:605257‑M2/156‑301 (MQ=255)                                                                                                                                               tgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGGTGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGccctttgatcaggttagatcggaagagg                                                                                                                                                          >  2:575734‑M2/153‑288 (MQ=255)                                                                                                                                                          aaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGAc                                                                                                                                               <  3:557816‑M2/160‑1 (MQ=255)                                                                                                                                                                     ggtggaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGtcct                                                                                                                                    <  1:182021‑M2/171‑1 (MQ=255)                                                                                                                                                                     cggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAgttgctctgcgccttgatcaggtttaccagagatcggaagagcacacgtctgaactcaa                                                                                                                                    >  4:765765‑M2/131‑272 (MQ=255)                                                                                                                                                                     cggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGCTCAGGTTTACCAGCTAGCCGGTCAGGTCGACGTTGGAgtccc                                                                                                                                    >  1:195577‑M2/131‑300 (MQ=255)                                                                                                                                                                         gaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAg                                                                                                                                <  3:195575‑M2/175‑1 (MQ=255)                                                                                                                                                                         gaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAg                                                                                                                                >  1:673‑M2/127‑301 (MQ=255)                                                                                                                                                                                         tgatagtacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATc                                                                                                                <  3:446501‑M2/191‑1 (MQ=255)                                                                                                                                                                                             agaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGc                                                                                                            <  1:605255‑M2/195‑1 (MQ=255)                                                                                                                                                                                                    gacgtgtgctcttccgatctgcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGt                                                                                                     <  4:252575‑M2/202‑1 (MQ=255)                                                                                                                                                                                                                gggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCgg                                                                                         >  4:697030‑M2/88‑301 (MQ=255)                                                                                                                                                                                                                gggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCgg                                                                                         <  2:696800‑M2/214‑1 (MQ=255)                                                                                                                                                                                                                        gcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACcggaaatctggccgagggtagatcggaagcgcgtcgggg                                                                                 >  2:252517‑M2/80‑282 (MQ=255)                                                                                                                                                                                                                          agaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCCCCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCgccggtc                                                                               >  2:771328‑M2/78‑298 (MQ=255)                                                                                                                                                                                                                           aaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCa                                                                              <  3:673‑M2/225‑1 (MQ=255)                                                                                                                                                                                                                            cagacgtgtgctcttccgatctaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCAACAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCAc                                                                             <  3:115790‑M2/226‑1 (MQ=255)                                                                                                                                                                                                                                      agcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGccc                                                                   <  3:563875‑M2/236‑1 (MQ=255)                                                                                                                                                                                                                                               aacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCGGAGGCATAg                                                          >  1:147865‑M2/57‑301 (MQ=255)                                                                                                                                                                                                                                                  aaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTAccggtgtccggagcgccgatcacagagcggaagagcgtcgtgtag                                                       >  1:115788‑M2/54‑279 (MQ=255)                                                                                                                                                                                                                                                         gtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGCGGCCGAGGACTCTTTcc                                                >  1:553392‑M2/47‑301 (MQ=255)                                                                                                                                                                                                                                                                       ttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGCAGCGCCGATCCCCGTCACGCCCGAGGCATAGGACTCTTTCCATTTGGTACCGCCa                                  >  4:687819‑M2/33‑301 (MQ=255)                                                                                                                                                                                                                                                                         cagtcgggtgaaccttcccattcttggggcCGCGTTGCGGCCCCAATTACCAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAAt                                <  2:687589‑M2/271‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aaccttaacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAgg                      <  3:470018‑M2/281‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGgcccc                  >  1:228973‑M2/17‑300 (MQ=255)                                                                                                                                                                                                                                                                                         cacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGc                >  3:462924‑M2/15‑301 (MQ=255)                                                                                                                                                                                                                                                                                          acattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTgcc               >  1:288215‑M2/14‑300 (MQ=255)                                                                                                                                                                                                                                                                                             ttcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTCCCGCCAATCGGCGTCAGGCCCTTCAGGt            >  2:116623‑M2/11‑301 (MQ=255)                                                                                                                                                                                                                                                                                             ttcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGt            <  3:288214‑M2/291‑1 (MQ=255)                                                                                                                                                                                                                                                                                             ttcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGt            <  4:771608‑M2/291‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    ggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGTTGGCGaa     >  3:345793‑M2/4‑301 (MQ=255)                                                                                                                                                                                                                                                                                                       cgcTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGGATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGTTGGCGAAGtt  >  1:534278/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                             GGGGCTTTCAGCGCGGCAAGGGGCAATAACCGTCAGTTTGTCGGCGGATGTTTGCCGCCTGCGGCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCACGCCCGAGGCATAGGACTCTTTCCAGTTGGTACCGCCAATCGGCGTCAGGCCCTGCAGGTTGGCGAAGTT  >  AE015451/4980295‑4980886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.