New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	2457220 =	88 (0.830)	4 (0.040)	4/582	8.2	2.5%	coding (291/999 nt)	PP_2150	putative Thiamine biosynthesis lipoprotein
	?	AE015451	= 5978413	224 (2.180)					coding (704/993 nt)	PP_5241	DNA‑binding response regulator, LuxR family
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTTCAGCTCGCCAGTGGCCTCGGCGACAAAACTGCTGACACCCATGGCCTGCAGGCGCGCAGCGATCAAGTCGACAGCATGACCGGCGACGATGCTGTTGAAGTCGAGCTGCACCGGGGCATCCTTGCACAGGGCTCGACCTTTGACGCGCAAGTGCTGGTAACCCACACGCTGGCGCACCTGGGCCAGTGCCTGTGGTTCGGGCACTTGCTCGTGACGGGCCTGAGGGCCGAAGCCCCACAAGTCGAGCAATGGCTCTACGGTGAGATCGAAAGCGCCATCGCTCTGCTCGGCAAGGTGCTGACCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/2457526‑2457220 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgctgacccTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGCAGAGGGGT  <  AE015451/5978413‑5978114                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 CTTCAGCTCGCCAGTGGCCTCGGCGACAAAACTGCTGACACCCATGGCCTGCAGGCGCGCAGCGATCAAGTCGACAGCATGACCGGCGACGATGCTGTTGAAGTCGAGCTGCACCGGGGCATCCTTGCACAGGGCTCGACCTTTGACGCGCAAGTGCTGGTAACCCACACGCTGGCGCACCTGGGCCAGTGCCTGTGGTTCGGGCACTTGCTCGTGACGGGCCTGAGGGCCGAAGCCCCACAAGTCGAGCAATGGCTCTACGGTGAGATCGAAAGCGCCATCGCTCTGCTCGGCAAGGTGC                                                                                                                                                                                                                                                                                                                    <  1:277999/301‑1                                                                                                                           CCTTGCACAGGGCTCGACCTTTGACGCGCAAGTGCTGGTAACCCACACGCTGGCGCACCTGGGCCAGTGCCTGTGGTTCGGGCACTTGCTCGTGACGGGCCTGAGGGCCGAAGCCCCACAAGTCGAGCAATGGCTCTACGGTGAGATCGAAAGCGCCATCGCTCTGCTCGGCAAGGTGCTGACCCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTG                                                                                                                                                                                          >  1:572110/1‑301                                                                                                                                           ACCTTTGACGCGCAAGTGCTGGTAACCCACACGCTGGCGAACCTGGGCCAGTGCCTGTGGTTCGGGCACTTGCTCGTGACGGGCCTGAGGGCCGAAGCCCCACAAGTCGAGCAATGGCTCTACGGTGAGATCGAAAGCGCCATCGCTCTGCTCGGCAAGGTGCTGACCCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGT                                                                                                                                                                          <  3:572122/301‑1                                                                                                                                                                                                                                                                                                      ccctGTGCTGACCCTGTTGCAGACAGGGCGGGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCA               <  2:846520/297‑1                                                                                                                                                                                                                                                                                                       gatcTGCTGACCCTGTTGCAGACAGGGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCAT              <  1:204687/297‑1                                                                                                                                                                                                                                                                                                          GTGCGGACCCGGTTGCAGACAGGGCGCGCCCGGGCCGGGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAG           <  2:313437/301‑1                                                                                                                                                                                                                                                                                                           TGCTGACCCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGg          >  3:636530/1‑300                                                                                                                                                                                                                                                                                                            GCTGACCCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATagatc         >  3:204694/1‑296                                                                                                                                                                                                                                                                                                            GCTGACCCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGCg         >  4:667451/1‑300                                                                                                                                                                                                                                                                                                            GCTGACCCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGCA         >  4:798530/1‑301                                                                                                                                                                                                                                                                                                              TGACCCTGTTGCAGCCAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGTGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGCAGA       <  3:300283/301‑1                                                                                                                                                                                                                                                                                                               GACCCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTATGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACCAGATGCCGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCCCAACCTCCTCGGTACGCGGCCTGCCATCCTGGtccgcccagtgcacggg      >  2:830176/1‑284                                                                                                                                                                                                                                                                                                               GACCCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGCAGAG      >  2:685455/1‑301                                                                                                                                                                                                                                                                                                                  CCTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGCAGAGGGG   >  1:292878/1‑301                                                                                                                                                                                                                                                                                                                   CTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGCAGAGGGGT  <  3:292883/301‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 CTTCAGCTCGCCAGTGGCCTCGGCGACAAAACTGCTGACACCCATGGCCTGCAGGCGCGCAGCGATCAAGTCGACAGCATGACCGGCGACGATGCTGTTGAAGTCGAGCTGCACCGGGGCATCCTTGCACAGGGCTCGACCTTTGACGCGCAAGTGCTGGTAACCCACACGCTGGCGCACCTGGGCCAGTGCCTGTGGTTCGGGCACTTGCTCGTGACGGGCCTGAGGGCCGAAGCCCCACAAGTCGAGCAATGGCTCTACGGTGAGATCGAAAGCGCCATCGCTCTGCTCGGCAAGGTGCTGACCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/2457526‑2457220 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgctgacccTGTTGCAGACAGTGCGCGCCCGGGCCGTGGCCGAAGGCCACAAGTGGCTGCAGGCGCGGCAGGTCGAGGAAAAGTACCAGCGCCTGACTCAGCGTGAGCGCCAGGTCTTCTCGCTGGTGGTAGGCGGCTTGTCGCACAAGCAGATCGCCAGGCAGATCGGCACCAGCGAGGTGACGACCAAGGTGCACAAGAAGAACATCATGAACAAGATGCAGTCCCGCTCGCTGCTGGAGCTTGCGGCCATGCACAACCTCCTCGGAGCGCGGTCTGCGAGCCTGGGCGGCGCATGAGCAGAGGGGT  <  AE015451/5978413‑5978114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.