New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5880817	240 (2.270)	4 (0.040)	4/600	8.4	1.4%	coding (422/1368 nt)	PP_5154	putative Oxidoreductase, FAD‑binding
	?	AE015451	= 5908358	311 (2.940)					coding (863/1098 nt)	spuD	spermidine/putrescine ABC transporter ‑ periplasmic subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAGGTCCAGCTTGCCCTGGAAGGCGTGCAGCACCGGCATGATCGAGTCGAAGTCCGGTGTGCCCAGCACCATGGCGGTGAGGTTGCGCGGTGCGCGGTCCAGGCGCATGGTGGCCTCGACCACGAAGCCCAGCGTACCCTCGGCACCAATGAACAGCTGGCGCAGGTCGTAGCCGGTGGCGTTCTTGATCAGGTCTTTGTTCAGCTCCAGCAACTCGCCCTTGCCGGTGACCACTTTCAGCCCGGCCACCCAGTTGCGGGTCATACCGTAACGAATCACCTTGATCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5880531‑5880817 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACGAGTTCATGAACTTCCTGATGCAGCCGGAAATCATGGCTGAAATCACCAACGCCGTGCGCTTTCCGAACGGTAACGCTGCTGCCACCCCGTTCGTGGACAAAGACATCACCAGCGACCCAAGCATCTATCCGCCTGCCGAAGTGAAGAAGCAGCTGTACGCGATCGCTGCGCCTGACGCTTCTGTGCAGCGAGTGATCACCCGCAGCTGGACGAAGATCAAGTCGGGCAAGTAAGCCCGATGCGACAGCTCTGGGCCGGGTTTTTCGGCCCAGAGCCATTGAAAGGTAATTGATGATT  <  AE015451/5908358‑5908059                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             CAGGTCCAGCTTGCCCTGGAAGGCGTGCAGCACCGGCATGATCGAGTCGAAGTCCGGTGTGCCCAGCACCATGGCGGTGAGGTTGCGCGGTGCGCGGTCCAGGCGCATGGTGGCCTCGACCACGAAGCCCAGCGTACCCTCGGCACCAATGAACAGCTGGCGCAGGTCGTAGCCGGTGGCGTTCTTGATCAGGTCTTTGTTCAGCTCCAGCAACTCGCCCTTGCCGGTGACCACTTTCAGCCCGGCCACCCAGTTGCGGGTCATACCGTAACGAATCACCTTGATCCAgatcggaagagca                                                                                                                                                                                                                                                                                                >  3:519756/1‑288                                                                                                                                                          CAGCTGGCGCAGGTCGTAGCCGGTGGCGTTCTTGATCAGGTCTTTGTTCAGCTCCAGCAACTCGCCCTTGCCGGTGACCACTTTCAGCCCGGCCACCCAGTTGCGGGTCATACCGTAACGAATCACCTTGATCCACGAGTTCATGAACTTCCTGATGCAGCCGGAAATCATGGCTGAAATCACCAACGCCGTGCGCTTTCCGAACGGTAACGCTGCTGCCACCCCGTTCGTGGACAAAGACATCACCAGCGACCCAAGCATCTATCCGCCTGCCGAAGTGAAGAAGCAGCTGTACCCGATC                                                                                                                                       >  2:656071/1‑301                                                                                                                                                                                                     TTGTTCAGCTCCAGCAACTCGCCCTTGCCGGTGACCACTTTCAGCCCGGCCACCCAGTTGCGGGTCATACCGTAACGAATCACCTTGATCCACGAGTTCATGAACTTCCTGATGCAGCCGGAAATCATGGCTGAAATCACCAACGCCGTGCGCTTTCCGAACGGTAACGCTGCTGCCACCCCGTTCGTGGACAAAGACATCACCAGCGACCCAAGCATCTATCCGCCTGCCGAAGTGAAGAAGCAGCTGTACGCGATCGCTGCGCCTGACGCTTCTGTGCAGCGAGTGATCACCCGCAGCT                                                                                            <  4:656292/301‑1                                                                                                                                                                                                                                                                                               CACGGGTTCATGAACTTCCTGATGCAGCCGGAAAGCATGGCTGAAATCACCAACGCCGTGCGCTTTCCGAACGGTAACGCTGCTGCCACCCCGTTCGTGGACAAAGACATCACCAGCGACCCAAGCATCTATCCGCCTGCCGAAGTGAAGAAGCAGCTGTACGCGATCGCTGCGCCTGACGCTTCTGTGCAGCGAGTGATCACCCGCAGCTGGACGAAGATCAAGTCGGGCAAGTAAGCCCGATGCGACAGCTCTGGGCCGGGTTTTTCGGCCCAGAGCCATTGAAAGGTAATTGATGATT  <  2:232326/301‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             CAGGTCCAGCTTGCCCTGGAAGGCGTGCAGCACCGGCATGATCGAGTCGAAGTCCGGTGTGCCCAGCACCATGGCGGTGAGGTTGCGCGGTGCGCGGTCCAGGCGCATGGTGGCCTCGACCACGAAGCCCAGCGTACCCTCGGCACCAATGAACAGCTGGCGCAGGTCGTAGCCGGTGGCGTTCTTGATCAGGTCTTTGTTCAGCTCCAGCAACTCGCCCTTGCCGGTGACCACTTTCAGCCCGGCCACCCAGTTGCGGGTCATACCGTAACGAATCACCTTGATCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5880531‑5880817 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ACGAGTTCATGAACTTCCTGATGCAGCCGGAAATCATGGCTGAAATCACCAACGCCGTGCGCTTTCCGAACGGTAACGCTGCTGCCACCCCGTTCGTGGACAAAGACATCACCAGCGACCCAAGCATCTATCCGCCTGCCGAAGTGAAGAAGCAGCTGTACGCGATCGCTGCGCCTGACGCTTCTGTGCAGCGAGTGATCACCCGCAGCTGGACGAAGATCAAGTCGGGCAAGTAAGCCCGATGCGACAGCTCTGGGCCGGGTTTTTCGGCCCAGAGCCATTGAAAGGTAATTGATGATT  <  AE015451/5908358‑5908059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.