Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	1,164,541	Δ873 bp		[yeaD]–[hexR]	[yeaD], [hexR]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	1164541	1165413	873	53 [0]	[0] 52	[yeaD]–[hexR]	[yeaD],[hexR]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1164540	0 (0.000)	52 (1.110)	51/530	0.1	100%	coding (847/855 nt)	yeaD	putative glucose‑6‑phosphate 1‑epimerase
	?	AE015451	1165414 =	0 (0.000)					intergenic (‑10/‑202)	hexR/zwfA	DNA‑binding transcriptional regulator/glucose 6‑phosphate‑1‑dehydrogenase

TGAAGATGGCGCGCTGAAGCTCAAGGGCGGCTGCCAGAAGGTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGTGAGGCCCAGCACCGAAGCGCCGTTTTCACGT  >  AE015451/1164263‑1164808                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                             tGAAGATGGCGCGCTGAAGCTCAAGGGCGGCTGCCAGAAGGTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTTCGCACCTGCGTTTGCAGGCGCACCGGCggctgtgtccttgggt                                                                                                                                                                                                                                                              >  2:414802‑M1/1‑278 (MQ=255)        ggCGCGCTGAAGCTCAAGGGCGGCTGCCAGAAGGTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacag                                                                                                                                                                                                                                                   <  1:113732‑M1/298‑28 (MQ=255)           gggctgAAGCTCAAGGGCGGCGGCCAGAAGGTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttg                                                                                                                                                                                                                                                                 <  1:458235‑M1/279‑14 (MQ=255)           gcgcTGAAGCTCAAGGGCGGCTGCCAGAAGGTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTTAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGc                                                                                                                                                                                                                                                                              >  2:458227/1‑268 (MQ=255)                gAAGCTCAAGGGCGGCTGCCAGAAGGTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgt                                                                                                                                                                                                                                         <  1:353226‑M1/300‑38 (MQ=255)                                        ggTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgca                                                                                                                                                                                                                      >  2:522316‑M1/1‑239 (MQ=255)                                        ggTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGGGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgca                                                                                                                                                                                                                      <  1:522326‑M1/295‑57 (MQ=255)                                         gTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagccggctttttgcacggtcg                                                                                                                                                                                                                >  1:167981‑M1/1‑238 (MQ=255)                                            tACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacaaccggctgtgtga                                                                                                                                                                                                                                       <  1:339692‑M1/274‑40 (MQ=255)                                            tACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttggg                                                                                                                                                                                                                                                               >  2:339684‑M1/1‑235 (MQ=255)                                                 cGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtc                                                                                                                                                                                                                 >  1:211827‑M1/1‑230 (MQ=255)                                                 cGGTACGCAACGGGTGCAATGGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtc                                                                                                                                                                                                                 <  2:211824‑M1/291‑62 (MQ=255)                                                           cGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcg                                                                                                                                                                                                                >  2:228997‑M1/1‑220 (MQ=255)                                                                   aTTGCAGGACCCGGCCTGGCAGCGCGAGCGGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatc                                                                                                                                                                                                          <  1:229005‑M1/280‑69 (MQ=255)                                                                         ggACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccac                                                                                                                                                                               >  1:119160‑M1/1‑206 (MQ=255)                                                                               ggCCGGGCAGCGCGGGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCGCCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttg                                                                                                                                                                            <  2:119157‑M1/298‑99 (MQ=255)                                                                                             aGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgt                                                                                                                                                            <  1:3544‑M1/300‑115 (MQ=255)                                                                                                 gtgCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtg                                                                                                                                                                                              <  1:13209‑M1/262‑81 (MQ=255)                                                                                                             cGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCTGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgcccttgggtcgggaagacagccggctgtgtgagcagcaggctttttg                                                                                                                                                                                                                        >  1:302113‑M1/1‑170 (MQ=255)                                                                                                                       gacgacACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgtt                                                                                                                                                    <  1:421712‑M1/282‑123 (MQ=255)                                                                                                                       gacgacACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaac                                                                                                                                                                    >  2:421704‑M1/1‑160 (MQ=255)                                                                                                                       gacgacACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaa                                                                                                                                                                     >  1:494621‑M1/1‑160 (MQ=255)                                                                                                                        acgacACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttg                                                                                                                                                                                                                        <  2:302105‑M1/213‑55 (MQ=255)                                                                                                                              ccGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaat                                                                                                                                                       >  1:287941‑M1/1‑153 (MQ=255)                                                                                                                                      tGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactttcggttcggttgacgtaccccttgtctgtaacacttgtgtgttatgttgtggtttttactacattttccctttaa                                                                                                                         >  2:101315‑M1/1‑145 (MQ=255)                                                                                                                                       ggCACCCTGGCACTCGCCCGCTGATGGGGGTGACCGGCCGCGGGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaa                                                                                                                                                                     <  2:494611‑M1/249‑106 (MQ=255)                                                                                                                                        gCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgt                                                                                                                                                                                    <  1:34063‑M1/233‑91 (MQ=255)                                                                                                                                        gCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgt                                                                                                                                                                                    >  2:34062‑M1/1‑143 (MQ=255)                                                                                                                                                              aTGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagacc                                                                                           <  1:101318‑M1/300‑180 (MQ=255)                                                                                                                                                                gggggTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaat                                                                                                                                                       <  2:287933‑M1/238‑120 (MQ=255)                                                                                                                                                                 ggggTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGTGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggttcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaac                                                                                        >  1:183613‑M1/1‑118 (MQ=255)                                                                                                                                                                       aCCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccccttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgggtgtgaaagcggtattcctagaccaactttagg                                                                                  >  1:449625‑M1/1‑112 (MQ=255)                                                                                                                                                                                    cGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtatt                                                                                                   >  2:243433‑M1/1‑99 (MQ=255)                                                                                                                                                                                    cGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtatt                                                                                                   <  1:243441‑M1/270‑172 (MQ=255)                                                                                                                                                                                     gCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatc                                                                    <  1:414810‑M1/300‑203 (MQ=255)                                                                                                                                                                                         cGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacgcttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagc                                                                                                         >  2:383025‑M1/1‑94 (MQ=255)                                                                                                                                                                                         cGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacgcttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagc                                                                                                         <  1:383033‑M1/259‑166 (MQ=255)                                                                                                                                                                                                   gCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgc                                                            >  2:526762‑M1/1‑84 (MQ=255)                                                                                                                                                                                                     gTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatcagtgt                                                   >  2:109914‑M1/1‑82 (MQ=255)                                                                                                                                                                                                             gACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgcgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgtaatcaggttcgaacctt                                           >  2:228875‑M1/1‑74 (MQ=255)                                                                                                                                                                                                                cagtggcagtggCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatccgtgtcgaacctt                                           >  2:75237‑M1/1‑71 (MQ=255)                                                                                                                                                                                                                cagtggcagtggCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatcagtgtcgaacctt                                           <  1:75240‑M1/298‑228 (MQ=255)                                                                                                                                                                                                                 agtggcagtggcGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaac                                                                                                                      >  1:503165‑M1/1‑70 (MQ=255)                                                                                                                                                                                                                       agtggcGAAGGCCTGGGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttagg                                                                                  <  2:167978‑M1/252‑189 (MQ=255)                                                                                                                                                                                                                       agtggcGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatcagtgtcgaaccttgcaccttt                                   >  1:167159‑M1/1‑64 (MQ=255)                                                                                                                                                                                                                             gAAGGCCTGAGCCTGGAACCTGGGAAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatc                                                        <  2:183610‑M1/272‑215 (MQ=255)                                                                                                                                                                                                                                ggCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatcagtgtcgaaccttgcacctttgccctgtttgg                        <  1:526773‑M1/301‑247 (MQ=255)                                                                                                                                                                                                                                       gCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatca                                                                   <  2:449617‑M1/251‑204 (MQ=255)                                                                                                                                                                                                                                       gCCTGGAACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatcagtgtcgaaccttgcacctt                                    <  1:228883‑M1/282‑235 (MQ=255)                                                                                                                                                                                                                                                                               gcACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatcagtgtcgaaccttgcacctttgccctgtttggcgccctcggcgacctggcattg  <  2:12964‑M1/276‑269 (MQ=255)                                                                                                                                                                                                                                                                               gcACCGGCgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatggccgcaatcagtgtcgaaccttgcacctttgccctgtttggcgccctcggcgacctggcattg  >  1:12965‑M1/1‑8 (MQ=255)                                                                                                                                                                                                                                                                                    ggcgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatg                                                                 <  1:520826‑M1/208‑206 (MQ=255)                                                                                                                                                                                                                                                                                    ggcgggtgtgtccttgggtcgggaagacagccggctgtgtgagcagcaggctttttgcacggtcgtctatcgtactgtcggtgcggttgacgtaccacttgtctgtaacacttgtgtgtaatgttgtggtttttactacattatcccttgaaaaccgtgtgtgaaagcggtattcctagaccaactttaggaaagaaccaacatcatg                                                                 >  2:520816‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                             TGAAGATGGCGCGCTGAAGCTCAAGGGCGGCTGCCAGAAGGTCTACCCCGGTACGCCACGGGTGCAATTGCAGGACCCGGCCTGGCAGCGCGAGCTGTGCATCGATACCGGTGACAGCGACGACACCGTGGTCTGGCACCCTGGCAATCGCCCGCTGATGGGGGTGACCGGCCGCGAGTCGCAGCGTTTTGTCTGCGTCGAGGCGACCAGTGGCAGTGGCGAAGGCCTGAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGTGAGGCCCAGCACCGAAGCGCCGTTTTCACGT  >  AE015451/1164263‑1164808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑