Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	1,164,541	Δ873 bp		[yeaD]–[hexR]	[yeaD], [hexR]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	1164541	1165413	873	53 [0]	[0] 52	[yeaD]–[hexR]	[yeaD],[hexR]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1164540	0 (0.000)	52 (1.110)	51/530	0.1	100%	coding (847/855 nt)	yeaD	putative glucose‑6‑phosphate 1‑epimerase
	?	AE015451	1165414 =	0 (0.000)					intergenic (‑10/‑202)	hexR/zwfA	DNA‑binding transcriptional regulator/glucose 6‑phosphate‑1‑dehydrogenase

TCGTCGGCCTCTACCGCGCGGCTGACATAGGCGGCACCACTGGCCAGGCTCTGCGCCAGTTGCAGCTTGAGTTCGGGGTAGCCGCTGACGCCGAACGAGCGGCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTG  >  AE015451/1165136‑1165681                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                            tgaagatggcgcgctgaagctcaagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgttcgcacctgcgtttgcaggcgcaccggcGGCTGTGTCCTTGGGt                                                                                                                                                                                                                                                              >  2:414802‑M2/279‑294 (MQ=255)        ggcgcgctgaagctcaagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAg                                                                                                                                                                                                                                                   <  1:113732‑M2/27‑1 (MQ=255)           gggctgaagctcaagggcggcggccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTg                                                                                                                                                                                                                                                                 <  1:458235‑M2/13‑1 (MQ=255)                gaagctcaagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCtgtgt                                                                                                                                                                                                                                         <  1:353226‑M2/37‑1 (MQ=255)                                        ggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCa                                                                                                                                                                                                                      >  2:522316‑M2/240‑295 (MQ=255)                                        ggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgggcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCa                                                                                                                                                                                                                      <  1:522326‑M2/56‑1 (MQ=255)                                         gtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCCGGCTTTTTGCACGgtcg                                                                                                                                                                                                                >  1:167981‑M2/239‑300 (MQ=255)                                            taccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTggg                                                                                                                                                                                                                                                               >  2:339684‑M2/236‑250 (MQ=255)                                            taccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAACCGGCTGTGTGa                                                                                                                                                                                                                                       <  1:339692‑M2/39‑1 (MQ=255)                                                 cggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGgtc                                                                                                                                                                                                                 >  1:211827‑M2/231‑291 (MQ=255)                                                 cggtacgcaacgggtgcaatggcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGgtc                                                                                                                                                                                                                 <  2:211824‑M2/61‑1 (MQ=255)                                                           cgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGgtcg                                                                                                                                                                                                                >  2:228997‑M2/221‑282 (MQ=255)                                                                   attgcaggacccggcctggcagcgcgagcggtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATc                                                                                                                                                                                                          <  1:229005‑M2/68‑1 (MQ=255)                                                                         ggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCAc                                                                                                                                                                               >  1:119160‑M2/207‑301 (MQ=255)                                                                               ggccgggcagcgcgggctgtgcatcgataccggtgacagcgacgacaccgtggtctggcgccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTg                                                                                                                                                                            <  2:119157‑M2/98‑1 (MQ=255)                                                                                             agctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTtgtgt                                                                                                                                                            <  1:3544‑M2/114‑1 (MQ=255)                                                                                                 gtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTg                                                                                                                                                                                              <  1:13209‑M2/80‑1 (MQ=255)                                                                                                             cggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggctgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGCCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTg                                                                                                                                                                                                                        >  1:302113‑M2/171‑224 (MQ=255)                                                                                                                       gacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTaa                                                                                                                                                                     >  1:494621‑M2/161‑265 (MQ=255)                                                                                                                       gacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAac                                                                                                                                                                    >  2:421704‑M2/161‑266 (MQ=255)                                                                                                                       gacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAtgtt                                                                                                                                                    <  1:421712‑M2/122‑1 (MQ=255)                                                                                                                        acgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTg                                                                                                                                                                                                                        <  2:302105‑M2/54‑1 (MQ=255)                                                                                                                              ccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAt                                                                                                                                                       >  1:287941‑M2/154‑272 (MQ=255)                                                                                                                                      tggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTTTCGGTTCGGTTGACGTACCCCTTGTCTGTAACACTTGTGTGTTATGTTGTGGTTTTTACTACATTTTccctttaa                                                                                                                         >  2:101315‑M2/146‑291 (MQ=255)                                                                                                                                       ggcaccctggcactcgcccgctgatgggggtgaccggccgcgggtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTaa                                                                                                                                                                     <  2:494611‑M2/105‑1 (MQ=255)                                                                                                                                        gcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGt                                                                                                                                                                                    >  2:34062‑M2/144‑233 (MQ=255)                                                                                                                                        gcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGt                                                                                                                                                                                    <  1:34063‑M2/90‑1 (MQ=255)                                                                                                                                                              atgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGAcc                                                                                           <  1:101318‑M2/179‑1 (MQ=255)                                                                                                                                                                gggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAt                                                                                                                                                       <  2:287933‑M2/119‑1 (MQ=255)                                                                                                                                                                 ggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggtgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTTCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAAc                                                                                        >  1:183613‑M2/119‑300 (MQ=255)                                                                                                                                                                       accggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCCCTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGGGTGTGAAAGCGGTATTCCTAGACCAACTTTAgg                                                                                  >  1:449625‑M2/113‑300 (MQ=255)                                                                                                                                                                                    cgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTAtt                                                                                                   <  1:243441‑M2/171‑1 (MQ=255)                                                                                                                                                                                    cgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTAtt                                                                                                   >  2:243433‑M2/100‑270 (MQ=255)                                                                                                                                                                                     gcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAAcatc                                                                    <  1:414810‑M2/202‑1 (MQ=255)                                                                                                                                                                                         cgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACGCTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGc                                                                                                         >  2:383025‑M2/95‑259 (MQ=255)                                                                                                                                                                                         cgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACGCTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGc                                                                                                         <  1:383033‑M2/165‑1 (MQ=255)                                                                                                                                                                                                   gcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGc                                                            >  2:526762‑M2/85‑294 (MQ=255)                                                                                                                                                                                                     gtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAgtgt                                                   >  2:109914‑M2/83‑301 (MQ=255)                                                                                                                                                                                                             gaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGCGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGTAATCAGGTTCGAACCtt                                           >  2:228875‑M2/75‑301 (MQ=255)                                                                                                                                                                                                                cagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCCGTGTCGAACCtt                                           >  2:75237‑M2/72‑298 (MQ=255)                                                                                                                                                                                                                cagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCtt                                           <  1:75240‑M2/227‑1 (MQ=255)                                                                                                                                                                                                                 agtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAAc                                                                                                                      >  1:503165‑M2/71‑222 (MQ=255)                                                                                                                                                                                                                       agtggcgaaggcctgggcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAgg                                                                                  <  2:167978‑M2/188‑1 (MQ=255)                                                                                                                                                                                                                       agtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCttt                                   >  1:167159‑M2/65‑299 (MQ=255)                                                                                                                                                                                                                             gaaggcctgagcctggaacctgggaagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATc                                                        <  2:183610‑M2/214‑1 (MQ=255)                                                                                                                                                                                                                                ggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTgg                        <  1:526773‑M2/246‑1 (MQ=255)                                                                                                                                                                                                                                       gcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAAcatca                                                                   <  2:449617‑M2/203‑1 (MQ=255)                                                                                                                                                                                                                                       gcctggaacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCtt                                    <  1:228883‑M2/234‑1 (MQ=255)                                                                                                                                                                                                                                                                               gcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTg  >  1:12965‑M2/9‑276 (MQ=255)                                                                                                                                                                                                                                                                               gcaccggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTg  <  2:12964‑M2/268‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATg                                                                 <  1:520826‑M2/205‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ggcGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATg                                                                 >  2:520816‑M2/4‑208 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                            TCGTCGGCCTCTACCGCGCGGCTGACATAGGCGGCACCACTGGCCAGGCTCTGCGCCAGTTGCAGCTTGAGTTCGGGGTAGCCGCTGACGCCGAACGAGCGGCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTG  >  AE015451/1165136‑1165681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑