Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	2,032,505	1680 bp→GCGGCCGC		[pgi‑I]	[pgi‑I]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	2032505	2034184	1680	71 [0]	[0] 75	[pgi‑I]	[pgi‑I]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 2032504	0 (0.000)	71 (0.850) +GCGGCCGC	66/536	0.5	100%	intergenic (‑260/‑16)	kdsA‑II/pgi‑I	2‑dehydro‑3‑deoxyphosphooctonate aldolase/glucose‑6‑phosphate isomerase
	?	AE015451	2034185 =	0 (0.000)					intergenic (+1/+1440)	pgi‑I/PP_5477	glucose‑6‑phosphate isomerase/conserved protein of unknown function

GTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCAAGGATCTTTTTGCAATGGCGTTCTACCGTACACCTCACGATGTCACTGCTCTGCCAGCCTGGAAGGCGCTTCAGCAACACCGCGAAGCCATGCGAGGCTTCAGCATGTCCGAAGCCTTTGCCGCCGATGCCAAGCGCTTCGACCAGTTTTCCCTTAGCGCTTGCGGCCTGTTCCTCGACTACTCAAAAAACCTGATCACCGAGCAAAGCCGCGATCTGCTGGTCAACCTGGCCAATGAAGTGGGCCTGCAGGACGCCATCAAGTCGATGTTCAGCGGTGAGATCATCAACGC  >  AE015451/2032225‑2032797                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      gtgGAGTTGATCTTGATCATTGTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTACCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgata                                                                                                                                                                                                                                                                                   <  2:41974‑M1/300‑21 (MQ=255)         gatcttgatcATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccg                                                                                                                                                                                                                                                                           >  1:51826‑M1/1‑272 (MQ=255)                       tttACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtc                                                                                                                                                                                                                                                             <  1:527033‑M1/300‑43 (MQ=255)                         tACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtcc                                                                                                                                                                                                                                                            >  2:737566‑M1/1‑256 (MQ=255)                                                            aTGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcg                                                                                                                                                                                                                           <  2:51824‑M1/297‑77 (MQ=255)                                                              ggCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccgg                                                                                                                                                                                                                                                                          >  2:726047‑M1/1‑219 (MQ=255)                                                                  aaGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcg                                                                                                                                                                                                                 <  2:607112‑M1/301‑87 (MQ=255)                                                                  aaGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcccatcatggc                                                                                                                                                                                                                  >  2:180134‑M1/1‑215 (MQ=255)                                                                    gggATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcgg                                                                                                                                                                                                                >  1:862477‑M1/1‑213 (MQ=255)                                                                      gATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcggg                                                                                                                                                                                                                                                                      >  1:337904‑M1/1‑211 (MQ=255)                                                                                   gcgcCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgctt                                                                                                                                                                                                                                                                                       >  2:939751‑M1/1‑198 (MQ=255)                                                                                   gcgcCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgctt                                                                                                                                                                                                                                                                                       <  1:939777‑M1/214‑17 (MQ=255)                                                                                       ccTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttc                                                                                                                                                                                            <  1:753431‑M1/301‑108 (MQ=255)                                                                                       ccTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCacggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaataggaggccgcattt                                                                                                                                                                                             >  2:890718‑M1/1‑194 (MQ=255)                                                                                                  gTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGGATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgc                                                                                                                                                                                       >  2:961883‑M1/1‑183 (MQ=255)                                                                                                     gTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtgg                                                                                                                                                                               >  1:362315‑M1/1‑180 (MQ=255)                                                                                                        gtgCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagtaggccgcatttcgacgcagaggtggtac                                                                                                                                                                            <  1:585911‑M1/300‑124 (MQ=255)                                                                                                            cGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggc                                                                                                                                                                                                                  >  1:772001‑M1/1‑173 (MQ=255)                                                                                                                tCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatag                                                                                                                                                                                                         <  2:862451‑M1/263‑95 (MQ=255)                                                                                                                 cTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagg                                                                                                                                                                  >  2:21151‑M1/1‑168 (MQ=255)                                                                                                                         tttCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtaca                                                                                                                                                                           <  2:771975‑M1/284‑125 (MQ=255)                                                                                                                           tCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtaccgtcaaccgggtaggtaatt                                                                                                                                                        >  1:248031‑M1/1‑158 (MQ=255)                                                                                                                                 gcCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCATGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcag                                                                                                                                                   <  1:658797‑M1/300‑149 (MQ=255)                                                                                                                                     ttCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGGATGAATGCGGCTTTTACCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagat                                                                                                                                                 <  1:961909‑M1/298‑151 (MQ=255)                                                                                                                                        gAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattt                                                                                                                                               >  1:227958‑M1/1‑145 (MQ=255)                                                                                                                                          gCATGGCGCGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaata                                                                                                                                                                                                          <  2:321447‑M1/236‑94 (MQ=255)                                                                                                                                            aTGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatcc                                                                                                                                       >  1:2375‑M1/1‑141 (MQ=255)                                                                                                                                            aTGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattt                                                                                                                                               >  1:573158‑M1/1‑141 (MQ=255)                                                                                                                                            aTGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattt                                                                                                                                               <  2:573136‑M1/293‑153 (MQ=255)                                                                                                                                            aTGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtc                                                                                                                                                                        >  1:303556‑M1/1‑141 (MQ=255)                                                                                                                                              ggCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattc                                                                                                                                                       <  2:248025‑M1/283‑145 (MQ=255)                                                                                                                                                 gAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggcggtcgccggaaacactagcgcatcatggcggtgaatagcaggc                                                                                                                                                                                                    >  1:706166‑M1/1‑136 (MQ=255)                                                                                                                                                  aGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaatttgcagattttgtcatccatcc                                                                                                                                   >  1:616136‑M1/1‑135 (MQ=255)                                                                                                                                                  aGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatcca                                                                                                                                  >  1:190957‑M1/1‑135 (MQ=255)                                                                                                                                                   ggCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaa                                                                                                                                 <  2:190953‑M1/300‑167 (MQ=255)                                                                                                                                                     cTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggt                                                                                                                                                                                                               >  2:738657‑M1/1‑132 (MQ=255)                                                                                                                                                     cTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggt                                                                                                                                                                                                               <  1:738683‑M1/220‑89 (MQ=255)                                                                                                                                                       gCCAGGCTTTTGTGGGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGAATTGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtg                                                                                                                              <  2:706141‑M1/299‑170 (MQ=255)                                                                                                                                                       gCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatcca                                                                                                                                  >  1:568235‑M1/1‑130 (MQ=255)                                                                                                                                                         cAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgct                                                                                                                            >  2:140927‑M1/1‑128 (MQ=255)                                                                                                                                                          aGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaa                                                                                                                          <  2:568214‑M1/300‑174 (MQ=255)                                                                                                                                                             cGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgc                                                                                                                       <  2:362303‑M1/300‑177 (MQ=255)                                                                                                                                                              gTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagt                                                                                                                               >  2:220597‑M1/1‑123 (MQ=255)                                                                                                                                                                ttGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctc                                                                                                                     <  2:2375‑M1/299‑179 (MQ=255)                                                                                                                                                                ttGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattt                                                                                                                                               <  2:227953‑M1/273‑153 (MQ=255)                                                                                                                                                                    gAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccgtccaagtgctaatgctcttgg                                                                                                                 >  1:178824‑M1/1‑117 (MQ=255)                                                                                                                                                                    gAGCGGTATTTTGCGCGAAGGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCacggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtg                                                                                                                              <  1:890744‑M1/286‑170 (MQ=255)                                                                                                                                                                       cGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcggaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatgg                                                                                                             <  1:180138‑M1/300‑187 (MQ=255)                                                                                                                                                                        ggTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggg                                                                                                            <  1:140930‑M1/300‑188 (MQ=255)                                                                                                                                                                                 cgcgcATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaaccgggtaggtaattcgcagattttgtcatccatccaagtgctaattctcttggaatgggctcagtccc                                                                                                  >  2:854040‑M1/1‑104 (MQ=255)                                                                                                                                                                                   cgcATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtccc                                                                                                  >  1:135052‑M1/1‑102 (MQ=255)                                                                                                                                                                                     cATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgaatttcgacgcagaggtggtacagt                                                                                                                                                                         >  1:410060‑M1/1‑100 (MQ=255)                                                                                                                                                                                         aaTGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggttaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagcttttgtcatccatccaagtgctaatgc                                                                                                                       >  2:827468‑M1/1‑96 (MQ=255)                                                                                                                                                                                         aaTGCGGCTTTTCCCTTTAGTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgagactggcccggcgggaggagcgtccgagcggagctggtggacgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtg                                                                                           <  1:827494‑M1/300‑205 (MQ=255)                                                                                                                                                                                               gCTTTTCCCTGTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgggcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctct                                                                                                                    <  1:230269‑M1/269‑180 (MQ=255)                                                                                                                                                                                                  tttCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagagg                                                                                   <  1:21153‑M1/299‑213 (MQ=255)                                                                                                                                                                                                           cTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcagcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtccc                                                                                                  >  1:478335‑M1/1‑78 (MQ=255)                                                                                                                                                                                                              gcgcATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtac                                                                       <  1:854066‑M1/299‑225 (MQ=255)                                                                                                                                                                                                                       cATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgcta                                                                                                                           <  1:256397‑M1/238‑173 (MQ=255)                                                                                                                                                                                                                       cATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgcta                                                                                                                           >  2:256391‑M1/1‑66 (MQ=255)                                                                                                                                                                                                                               ataATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgct                                                                                                                      <  2:303548‑M1/235‑178 (MQ=255)                                                                                                                                                                                                                                       gCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgca                                               <  2:135049‑M1/298‑249 (MQ=255)                                                                                                                                                                                                                                          gACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgctggatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaatt                                            <  2:337893‑M1/298‑252 (MQ=255)                                                                                                                                                                                                                                                            gATTGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaatt                                            >  2:572776‑M1/1‑29 (MQ=255)                                                                                                                                                                                                                                                               tGATAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttaccgg                                 >  2:446777‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                                                  tAGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttaccggatgcgctgcgtcctc                  >  1:561519‑M1/1‑23 (MQ=255)                                                                                                                                                                                                                                                                   aGGTTCTGGCTTTACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcaccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcc                                                                                                   <  1:805598‑M1/218‑197 (MQ=255)                                                                                                                                                                                                                                                                              ttACCCGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttaccggatgcgctgcgtcctcggaaatgaagac      <  1:737592‑M1/300‑290 (MQ=255)                                                                                                                                                                                                                                                                               tACCCGTATCgctgccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgact                                                                                        <  2:410041‑M1/217‑208 (MQ=255)                                                                                                                                                                                                                                                                                 cccGTATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttaccggatgcgctgcgtcctcggaaatgaagacggt   <  1:220602‑M1/300‑293 (MQ=255)                                                                                                                                                                                                                                                                                     tATCgcggccgcccctgcttgatactggcccggcgggaggagcgtccgagcggagctggtggtcgccggaaacactagcgcatcatggcggtgaatagcaggccgcatttcgacgcagaggtggtacagtcaacagggtaggtaattcgcagattttgtcatccatccaagtgctaatgctcttggatggggctcagtcccagttgtgactagaggatctgaatgtacaatgcacttctgctaatcgctgcaattgcccttaccggatgcgctgcgtcctcggaaatgaagacggtg  >  2:688616‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      GTGGAGTTGATCTTGATCATTTTTTACTCGATAGGTCCTTAGGGGGCAACCCGCAGGACATGGCAAAGGGATAATAGTCAGCGCGCCCTTGCTAACCGTGGTTGTGCCGATTCTACAATTTTTCTGCTGCCTTTCGAGCATGGCGAGGCTGCCAGGCGTTTGTGAGCGGTATTTTGCGCGCATGAATGCGGCTTTTCCCTTTCTTGCGCATGGGCATTGCCTATAATGTCGCCGACGTGGAACCTGACAACGATTGATAGGTTCTGGCTTTACCCGTATCAAGGATCTTTTTGCAATGGCGTTCTACCGTACACCTCACGATGTCACTGCTCTGCCAGCCTGGAAGGCGCTTCAGCAACACCGCGAAGCCATGCGAGGCTTCAGCATGTCCGAAGCCTTTGCCGCCGATGCCAAGCGCTTCGACCAGTTTTCCCTTAGCGCTTGCGGCCTGTTCCTCGACTACTCAAAAAACCTGATCACCGAGCAAAGCCGCGATCTGCTGGTCAACCTGGCCAATGAAGTGGGCCTGCAGGACGCCATCAAGTCGATGTTCAGCGGTGAGATCATCAACGC  >  AE015451/2032225‑2032797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑