Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	1,709,370	2640 bp→CGGCCGC		[ppc]	[ppc]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	1709370	1712009	2640	53 [0]	[0] 54	[ppc]	[ppc]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1709369	0 (0.000)	52 (0.620) +CGGCCGC	49/538	1.4	100%	intergenic (‑136/‑13)	PP_1504/ppc	conserved protein of unknown function/Phosphoenolpyruvate carboxylase
	?	AE015451	1712010 =	0 (0.000)					intergenic (+1/‑257)	ppc/adk	Phosphoenolpyruvate carboxylase/Adenylate kinase

ACCGCACCTGCGTCCTTTAGGTGCGCACCTGCGCGACCTATTGTCGCAGTCATGCCAGGTGGTGCTGGGCCTGACCGGGCAGCCGGTGCTACTGGCGCACAGCCCCGAGACACTGGAATTCATTAGCCTGCGCAATACCTATCTGGACCCGTTGCACCGCCTGCAGGCAGAGTTGCTGGCCCGCTCGCGCAGCCGCGAAGCCGCTCTGGACAGCCCGTTGGAGCAGGCCTTGCTGGTGACCGTGGCGGGTATTGCCGCCGGGCTGCGCAACACCGGCTAAGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGAGGAGCACAAGATGCGCGTAATTCTGCTGGGAGCTCCCGGGGCCGGTAAAGGTA  >  AE015451/1711730‑1712309                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                             aggcgcgcttcggactccaggtccaggtgcaggacatgaccggtacggctgagggtaccggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTc                                                                                                                                                                                                                                                                                           >  1:189905‑M2/281‑300 (MQ=255)  ggcgcgcttcggactccaggtccaggtgcaggacatgaccggtacggctgagggtaccggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTg                                                                                                                                                                                                                                                                                         <  1:906588‑M2/22‑1 (MQ=255)  ggcgcgcttcggactccaggtccaggtgcaggacatgaccggtacggctgagggtaccggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttgaccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCt                                                                                                                                                                                                                                                                                          <  1:557487‑M2/21‑1 (MQ=255)            ggactccaggtccaggtgcaggacatgaccggtacggctgagggtaccggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGcc                                                                                                                                                                                                                                                                                >  1:604659‑M2/270‑300 (MQ=255)                   aggtccaggtgcaggacatgaccggtacggctgagggtaccggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAgg                                                                                                                                                                                                                                                                         >  1:895962‑M2/263‑300 (MQ=255)                   aggtccaggtgcaggacatgaccggtacggctgagggtaccggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAg                                                                                                                                                                                                                                                                          <  2:117042‑M2/37‑1 (MQ=255)                               aggacatgaccggtacggctgagggtaccggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAgg                                                                                                                                                                                                                                                             >  1:36782‑M2/251‑300 (MQ=255)                                          ggtacggctgagggtaccggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCg                                                                                                                                                                                                                                                 >  1:170124‑M2/240‑301 (MQ=255)                                                            ggcggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGgc                                                                                                                                                                                                                                     >  1:86520‑M2/222‑295 (MQ=255)                                                               ggcattctgttcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgaactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGgc                                                                                                                                                                                                                                     <  2:86518‑M2/74‑1 (MQ=255)                                                                         tcccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTc                                                                                                                                                                                                                   >  1:232211‑M2/209‑300 (MQ=255)                                                                          cccagtgctgggcgagttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgataacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCt                                                                                                                                                                                                                           <  2:36780‑M2/84‑1 (MQ=255)                                                                                          ttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTTACGGCCATACAGGCCCGGCCCAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCgg                                                                                                                                                                                                                     >  2:245235‑M2/192‑281 (MQ=255)                                                                                          ttcacggattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCgg                                                                                                                                                                                                                     <  1:245241‑M2/90‑1 (MQ=255)                                                                                                gattttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCACAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGc                                                                                                                                                                                            <  2:604634‑M2/115‑1 (MQ=255)                                                                                                  ttttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCt                                                                                                                                                                                              >  1:105533‑M2/184‑296 (MQ=255)                                                                                                   tttcatgatgacctccgtggacgcctggaaaggctgcactgaatgggagtgctgtgctttcactttagtttggtttggccggtcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgaacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTACCCGGTCAGCCGCAAGTGGCGCATTCCTGCAAc                                                                                                                                                                                         <  2:625835‑M2/118‑1 (MQ=255)                                                                                                    ttcatgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCGCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAAcc                                                                                                                                                                                                                               >  2:490522‑M2/182‑261 (MQ=255)                                                                                                    ttcatgatgacctctgtggactcctggaaaggctgacctgcatgggagtgcttggcttgcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCAGCAACt                                                                                                                                                                                        <  1:490543‑M2/119‑1 (MQ=255)                                                                                                        tgatgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTTCGCCAGGCGCAACCGCCTGCCCTGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTggg                                                                                                                                                                                   >  2:723508‑M2/178‑301 (MQ=255)                                                                                                          atgacctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTTCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTg                                                                                                                                                                                                        >  1:752338‑M2/176‑278 (MQ=255)                                                                                                             acctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCCCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTggg                                                                                                                                                                                   >  2:274303‑M2/173‑296 (MQ=255)                                                                                                              cctctgttgacgcctggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCtt                                                                                                                                                                             <  1:274310‑M2/130‑1 (MQ=255)                                                                                                                             ggaaaggctgcactgcatgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTcc                                                                                                                                                                                               >  2:557468‑M2/157‑268 (MQ=255)                                                                                                                             ggaaaggctgcactgaatgtgagtgctgtgctttaactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCt                                                                                                                                                                                              <  2:196‑M2/113‑1 (MQ=255)                                                                                                                                             atgggagtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGTCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGgc                                                                                                                                                              >  2:459388‑M2/141‑285 (MQ=255)                                                                                                                                                  agtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTg                                                                                                                                                                    >  2:207813‑M2/136‑274 (MQ=255)                                                                                                                                                  agtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTg                                                                                                                                                                    <  1:207818‑M2/139‑1 (MQ=255)                                                                                                                                                   gtgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTggg                                                                                                                                                                                   >  1:434601‑M2/135‑258 (MQ=255)                                                                                                                                                    tgctgtgctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTG‑GTGATCTTTAGCAGCCtt                                                                                                                                       >  2:5381‑M2/134‑300 (MQ=255)                                                                                                                                                          gctttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTc                                                                                                                                      >  1:132523‑M2/128‑295 (MQ=255)                                                                                                                                                          gctttcactttagtttggtttggccgatcacggcttgccagcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTc                                                                                                                                      <  2:132520‑M2/168‑1 (MQ=255)                                                                                                                                                             ttcactttagtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGTCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGa                                                                                                                               <  1:459409‑M2/175‑1 (MQ=255)                                                                                                                                                                     agtttggtttggccgatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTc                                                                                                                      <  1:5383‑M2/184‑1 (MQ=255)                                                                                                                                                                                   gatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCGACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑TTGTTGACGAGCCTTCTTGCCTATTTAGGGTCATACCCGATTTTcc                                                                                                        >  2:760026‑M2/103‑300 (MQ=255)                                                                                                                                                                                   gatcacggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACGTTGGGACGCTTGTTTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCtt                                                                                                                                       >  1:200376‑M2/103‑269 (MQ=255)                                                                                                                                                                                         ggcttgccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGagcag                                                                                                                                           >  1:663971‑M2/97‑259 (MQ=255)                                                                                                                                                                                         ggcttgcaatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGagcag                                                                                                                                           <  2:663946‑M2/163‑1 (MQ=255)                                                                                                                                                                                              gccatagtcgctcggctgtgggaactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGcc                                                                                              <  2:170120‑M2/208‑1 (MQ=255)                                                                                                                                                                                               ccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGTGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATTGTCATACCCGATTTTCCAGACTTGGCCCt                                                                                            >  2:483415‑M2/91‑300 (MQ=255)                                                                                                                                                                                               ccatcgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCt                                                                                            >  1:364503‑M2/91‑300 (MQ=255)                                                                                                                                                                                                   cgtcgctcggctgtgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggaggcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGAACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGcc                                                                                              <  1:760052‑M2/208‑1 (MQ=255)                                                                                                                                                                                                                tgggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTTGCCCTTGTCGCCGAATCCAtt                                                                            >  1:732030‑M2/74‑299 (MQ=255)                                                                                                                                                                                                                 gggcactcttgggggataccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTg                                                                                                                                                <  2:232206‑M2/158‑1 (MQ=255)                                                                                                                                                                                                                                   ccgctcgctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTg                                                        >  1:503494‑M2/55‑300 (MQ=255)                                                                                                                                                                                                                                        cgatcctgcacagggcgcgatgagcctgatagggatgcaaagcggccgcGTGCAAGCCCGGGTCATGTCTGGGGACGGCGAAACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTg                                                                                                                                                                                                        <  2:752312‑M2/103‑1 (MQ=255)                                                                                                                                                                                                                                         gctcctgcacagggcgcgctgaggctgatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGAGGAGc                                                  >  1:201291‑M2/49‑300 (MQ=255)                                                                                                                                                                                                                                               gcacagggcgcgctgaggctgttagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCtt                                                                                                                                       <  2:189901‑M2/167‑1 (MQ=255)                                                                                                                                                                                                                                                                   gatagggatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGAGGAGCACAAGATgc                                         >  1:524362‑M2/23‑283 (MQ=255)                                                                                                                                                                                                                                                                         gatgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGCGGAGCACAAGATGCGCGTAATTCTGCTGGGAGCTcc                   >  1:214850‑M2/17‑299 (MQ=255)                                                                                                                                                                                                                                                                          atgcaacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGAGGAGCACAAGATTCGCGTAAtt                                 >  2:869859‑M2/16‑284 (MQ=255)                                                                                                                                                                                                                                                                              aacgcggccgcGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGAGGAGCACAAGATGCGTGTAATTCTGCTGGGAGCTCCCGGGGcc            <  1:723534‑M2/290‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gggCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGTGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCAGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGAGGAGCACAAGATGCGCGTAATTCTGCTGGGAGCTCCCGGGGCCGGTAAAGGTa  <  1:483436/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gggCAAGCCCGGGTAATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGAGGAGCACAAGATGCGCGTAATTCTGCTGGGAGCTCCCGGGGCCGGTAAAGGTa  <  2:105531/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                             ACCGCACCTGCGTCCTTTAGGTGCGCACCTGCGCGACCTATTGTCGCAGTCATGCCAGGTGGTGCTGGGCCTGACCGGGCAGCCGGTGCTACTGGCGCACAGCCCCGAGACACTGGAATTCATTAGCCTGCGCAATACCTATCTGGACCCGTTGCACCGCCTGCAGGCAGAGTTGCTGGCCCGCTCGCGCAGCCGCGAAGCCGCTCTGGACAGCCCGTTGGAGCAGGCCTTGCTGGTGACCGTGGCGGGTATTGCCGCCGGGCTGCGCAACACCGGCTAAGGGCAAGCCCGGGTCATGTCTGGTGACGGCCATACAGGCCCGGCACAAGGACGGGGAGGGCGGTTGCGCCAGGCGCAACCGCCTGCCCGGTCAGCCGCAAGTGGCGCATTCCTGCAACTTTGGGACGCTTGTCTGGCTGCCGGGCGCTGTGT‑ATCTTGAGCAGCCTTCTGACCGATTTATGGTCATACCCGATTTTCCGGACTTGGCCCTGGTCGCCGAATCCATTGAATTTCATACAAAAATTTGAGGAGCACAAGATGCGCGTAATTCTGCTGGGAGCTCCCGGGGCCGGTAAAGGTA  >  AE015451/1711730‑1712309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑