Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	5,342,745	1681 bp→GCGGCCGC		[pgi‑II]	[pgi‑II]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	5342745	5344425	1681	53 [0]	[0] 53	[pgi‑II]	[pgi‑II]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5342744	0 (0.000)	52 (0.620) +GCGGCCGC	49/536	1.4	100%	intergenic (+190/‑17)	panC/pgi‑II	pantothenate synthetase/glucose‑6‑phosphate isomerase
	?	AE015451	5344426 =	0 (0.000)					intergenic (+1/‑177)	pgi‑II/acsA‑II	glucose‑6‑phosphate isomerase/acetyl‑CoA synthetase

AAGCTGGCCCCGCACAAGGTGATCCCGGGTAACCGCCCGAGCAACACGTTGGTGGTCGAGCGCATCAGCCCGCGCCGGCTGGGTGCGCTGGTGGCCATGTACGAGCACAAGGTGTTCGTACAGAGCGTTATCTGGGGTATCAACGCCTTCGATCAGTGGGGCGTCGAGCTGGGCAAGGAGCTGGGCAAGGGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGACCCAGACACCTTCTGG  >  AE015451/5344138‑5344719                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                      cgagacctggtggtgattgcggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTtgt                                                                                                                                                                                                                                                                                             <  1:155569‑M2/11‑1 (MQ=255)         ggtggtgattgcggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCctct                                                                                                                                                                                                                                                                                   <  1:39146‑M2/21‑1 (MQ=255)                     ggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTc                                                                                                                                                                                                                                                                                                      <  1:625899‑M2/2‑1 (MQ=255)                     ggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTc                                                                                                                                                                                                                                                                                                      >  2:625874‑M2/269‑270 (MQ=255)                     ggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTtgt                                                                                                                                                                                                                                                                                             <  1:24958‑M2/11‑1 (MQ=255)                       cggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactcccctgtccaagccctcaccacgcaccccggccccGCCGAGCTTGTGCCCGTCTCTTCGCCGGTAAAccc                                                                                                                                                                                                                                                                     >  2:371656‑M2/267‑301 (MQ=255)                        ggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGcc                                                                                                                                                                                                                                                                                      >  1:692061‑M2/266‑283 (MQ=255)                            tacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCgg                                                                                                                                                                                                                                                                             >  1:490312‑M2/262‑288 (MQ=255)                            tacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                                                                                    <  1:63785‑M2/36‑1 (MQ=255)                                 gggtaacacgcggtggttcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCa                                                                                                                                                                                                                                                             <  1:371668‑M2/43‑1 (MQ=255)                                    taacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAg                                                                                                                                                                                                                                                                                                  <  1:139194‑M2/6‑1 (MQ=255)                                    taacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGc                                                                                                                                                                                                                                                          >  1:63201‑M2/254‑299 (MQ=255)                                    taacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgcccctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctcaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCgat                                                                                                                                                                                                                                                                                                  >  2:139191‑M2/254‑258 (MQ=255)                                         cgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtctttggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAAcg                                                                                                                                                                                                                                                      <  2:63199‑M2/50‑1 (MQ=255)                                                     acaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtttccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCt                                                                                                                                                                                                                                              >  2:827465‑M2/237‑294 (MQ=255)                                                         cctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtttccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTa                                                                                                                                                                                                                                          >  1:383325‑M2/233‑294 (MQ=255)                                                         cctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGGTACCCCGTAGGAGCgg                                                                                                                                                                                                                                   >  2:37699‑M2/233‑301 (MQ=255)                                                              acctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTa                                                                                                                                                                                                                                          <  2:383313‑M2/62‑1 (MQ=255)                                                                  gcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCt                                                                                                                                                                                                                                                                 <  1:499866‑M2/39‑1 (MQ=255)                                                                   catttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAccc                                                                                                                                                                                                                           >  1:140749‑M2/223‑299 (MQ=255)                                                                    atttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAAcc                                                                                                                                                                                                                                                                      <  2:490291‑M2/34‑1 (MQ=255)                                                                    atttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGttt                                                                                                                                                                                                                               <  1:827491‑M2/73‑1 (MQ=255)                                                                    atttggaagagaagaccgaataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAccc                                                                                                                                                                                                                           <  2:315078‑M2/77‑1 (MQ=255)                                                                        ggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcCCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGc                                                                                                                                                                                                                                                          >  2:890029‑M2/218‑263 (MQ=255)                                                                        ggaagagaagaccgaaaaacggtcttgggctgccttgcagcccatacgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagaacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAc                                                                                                                                                                                                                             <  1:174901‑M2/75‑1 (MQ=255)                                                                          aagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgccaataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACCAGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAgg                                                                                                                                                                                                                  >  1:157503‑M2/216‑301 (MQ=255)                                                                                   ccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGc                                                                                                                                                                                                                                                                   >  2:17211‑M2/207‑243 (MQ=255)                                                                                     gcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGc                                                                                                                                                                                                                                                                                           <  2:666925‑M2/13‑1 (MQ=255)                                                                                      cataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCCGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAggcc                                                                                                                                                                                                                >  1:615394‑M2/204‑291 (MQ=255)                                                                                                        ccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCa                                                                                                                                                                                        <  1:37701‑M2/112‑1 (MQ=255)                                                                                                            gcagcccattcgcgggcaagcccgctcctgcacgtgtacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAcc                                                                                                                                                                                                                            <  1:347218‑M2/76‑1 (MQ=255)                                                                                                             cagcccattcgcggccaggcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGtt                                                                                                                                                                                                      <  1:17213‑M2/98‑1 (MQ=255)                                                                                                                   attcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCCTTACCTCGTAGGAGCCGGTTTACCCGCGAAGAAGCCGGCCCAGGTTGAACACCGGTCCCAGCTACACTGTCtt                                                                                                                                                                           >  1:265139‑M2/175‑299 (MQ=255)                                                                                                                           gcaagcccgctcctgcacgtttacctgtaggtgcgggcttgcttgcaatgcgcccaaaatccccctgccatacccattcccagcacgtcgcctttgcctataacggtgccagcctgaacccggcaacgaccgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCtt                                                                                                                                                                                                                                                                                               <  2:371751‑M2/9‑1 (MQ=255)                                                                                                                             aagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGt                                                                                                                                                                                                       <  1:890055‑M2/97‑1 (MQ=255)                                                                                                                                           acgtttacctgtagggacgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATc                                                                                                                                                   <  1:770918‑M2/149‑1 (MQ=255)                                                                                                                                             gtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAggcc                                                                                                                                                                                                                <  2:615369‑M2/88‑1 (MQ=255)                                                                                                                                                tacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACCCTGTTCTATCGAATAACCGTTGCAGTCCAACGCCCCt                                                                                                                                             >  2:36842‑M2/146‑300 (MQ=255)                                                                                                                                                     gtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACAATGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCtt                                                                                                                                            >  1:204031‑M2/141‑296 (MQ=255)                                                                                                                                                             gggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTc                                                                                                                                                                                                                                             >  2:470362‑M2/133‑191 (MQ=255)                                                                                                                                                                      tgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCg                                                                                                                         <  2:140746‑M2/175‑1 (MQ=255)                                                                                                                                                                              ccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGt                                                                                                                   <  1:36844‑M2/181‑1 (MQ=255)                                                                                                                                                                                  aaaagccccctgccataccccttcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATcc                                                                                                          <  2:44843‑M2/190‑1 (MQ=255)                                                                                                                                                                                             gccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTGACCCGCGAAGAGGCCGGCCCAGGTTg                                                                                                                                                                                                     >  2:733150‑M2/101‑199 (MQ=255)                                                                                                                                                                                                   cccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAaca                                                                                                                                                                                                 <  2:692036‑M2/103‑1 (MQ=255)                                                                                                                                                                                                      attcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTAc                                                                                                                                          >  2:180523‑M2/92‑249 (MQ=255)                                                                                                                                                                                                                 gtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGCAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGt                                                                                                                                                                              >  2:246331‑M2/81‑202 (MQ=255)                                                                                                                                                                                                                     cctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATc                                                                                                           <  2:204026‑M2/189‑1 (MQ=255)                                                                                                                                                                                                                                         agcctgaacacggcaacgactgccgtgtccaagccctaaccacgaaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCAt                                                                                                                                                    <  1:470383‑M2/148‑1 (MQ=255)                                                                                                                                                                                                                                            ctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCCCACCCCCGa                                                      >  1:855137‑M2/54‑295 (MQ=255)                                                                                                                                                                                                                                                acccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTAcc                                              <  1:180527‑M2/250‑1 (MQ=255)                                                                                                                                                                                                                                                                       aagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGc                                                                                          <  1:898724‑M2/206‑1 (MQ=255)                                                                                                                                                                                                                                                                                          cggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGACCCAGACACCTTCTgg  >  1:664081‑M2/8‑301 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                      AAGCTGGCCCCGCACAAGGTGATCCCGGGTAACCGCCCGAGCAACACGTTGGTGGTCGAGCGCATCAGCCCGCGCCGGCTGGGTGCGCTGGTGGCCATGTACGAGCACAAGGTGTTCGTACAGAGCGTTATCTGGGGTATCAACGCCTTCGATCAGTGGGGCGTCGAGCTGGGCAAGGAGCTGGGCAAGGGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGACCCAGACACCTTCTGG  >  AE015451/5344138‑5344719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑