Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	1,164,572	830 bp→TCA	coding (4‑833/864 nt)	hexR ←	DNA‑binding transcriptional regulator

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	1164572	1165401	830	68 [0]	[0] 65	hexR	DNA‑binding transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1164571	0 (0.000)	65 (0.800) +TCA	61/594	0.2	100%	coding (834/864 nt)	hexR	DNA‑binding transcriptional regulator
	?	AE015451	1165402 =	0 (0.000)					coding (3/864 nt)	hexR	DNA‑binding transcriptional regulator

CGAAGATCTTCTGGGTGTAGGCGGCCGGGTCATCGTCGGCCTCTACCGCGCGGCTGACATAGGCGGCACCACTGGCCAGGCTCTGCGCCAGTTGCAGCTTGAGTTCGGGGTAGCCGCTGACGCCGAACGAGCGGCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCCT  >  AE015451/1165104‑1165696                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       ggaagctaaagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcacac                                                                                                                                                                                                                                                                                                      <  2:729744‑M2/3‑1 (MQ=255)        tcttccgatctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgctcctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTcc                                                                                                                                                                                                                                                                                               <  1:413791‑M2/10‑1 (MQ=255)           gggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatccCACGCGGTCCatt                                                                                                                                                                                                                                                                                            >  2:409815‑M2/289‑300 (MQ=255)               ggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGgtg                                                                                                                                                                                                                                                                                        >  1:426981‑M2/285‑301 (MQ=255)                   gccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgctcctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaaccgcggtccagatcggaagg                                                                                                                                                                                                                                                                                    >  2:413772‑M2/281‑291 (MQ=255)                           gtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGgtg                                                                                                                                                                                                                                                                            >  1:343088‑M2/273‑300 (MQ=255)                                         gccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCgg                                                                                                                                                                                                                                                              >  1:852821‑M2/259‑301 (MQ=255)                                              gggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCtgtg                                                                                                                                                                                                                                                         >  1:83674‑M2/254‑301 (MQ=255)                                                 tgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGctgtgtgg                                                                                                                                                                                                                                                      >  1:738980‑M2/251‑300 (MQ=255)                                                  gcaattgcaggacccggcctggcggcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtagtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGGCCTTGGGTCGGGAATACAGCCGGATGTGTGag                                                                                                                                                                                                                                                     >  2:412574‑M2/250‑301 (MQ=255)                                                   aaatggcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagctacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGagc                                                                                                                                                                                                                                                    <  2:247239‑M2/53‑1 (MQ=255)                                                         caggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTgagcagcatg                                                                                                                                                                                                                                              >  2:589238‑M2/243‑299 (MQ=255)                                                          aggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGTAAGACAGCCGGCTGtgggaggagccggg                                                                                                                                                                                                                                             >  1:388498‑M2/242‑297 (MQ=255)                                                            gacccggccgggcagcgcgagctgtgcatcgataccggtgacagcgacgaaaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCtt                                                                                                                                                                                                                                           <  2:655797‑M2/62‑1 (MQ=255)                                                             cccgggccgggaagcgcgggctgtgcatcgataccggtgaaagggacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCttt                                                                                                                                                                                                                                          <  2:515193‑M2/63‑1 (MQ=255)                                                             ccccggccgggaagcgcgagctgtgcatcgataccggggacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCttt                                                                                                                                                                                                                                          <  2:852798‑M2/63‑1 (MQ=255)                                                                 ggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcacagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGc                                                                                                                                                                                                                                      <  1:442074‑M2/67‑1 (MQ=255)                                                                    cttccgatctgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACg                                                                                                                                                                                                                                   <  2:709727‑M2/70‑1 (MQ=255)                                                                    ctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACg                                                                                                                                                                                                                                   >  1:328383‑M2/232‑301 (MQ=255)                                                                             cgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTAt                                                                                                                                                                                                                          >  2:519859‑M2/223‑301 (MQ=255)                                                                              gagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGctttttgcacgagatcggaag                                                                                                                                                                                                                         >  1:709749‑M2/222‑291 (MQ=255)                                                                                       accgataccggtgaaagcgacgaaaccggggtctggaacccgggaaatcgcccgctgatgggggtgaccggccgcgggtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCg                                                                                                                                                                                                                <  2:343077‑M2/89‑1 (MQ=255)                                                                                            gcccggtgacagggacgaacccgtggtcgggaaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgaacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCg                                                                                                                                                                                                           <  2:16270‑M2/94‑1 (MQ=255)                                                                                                     cagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTa                                                                                                                                                                                                  <  1:589260‑M2/103‑1 (MQ=255)                                                                                                      agcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTAc                                                                                                                                                                                                 >  1:223665‑M2/198‑301 (MQ=255)                                                                                                         gaggacaccgtggtctggcaccctggcaatggcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCAc                                                                                                                                                                                              <  2:269233‑M2/107‑1 (MQ=255)                                                                                                         gacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCAc                                                                                                                                                                                              >  1:778121‑M2/195‑301 (MQ=255)                                                                                                           cgcaaccgtggtctggcaccctggaaatagcccgctgatgggggtgaccggccgcgggtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCCATCGTACTGTCGGTGCGGTTGACGTACCACtt                                                                                                                                                                                            <  1:412593‑M2/109‑1 (MQ=255)                                                                                                            gacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCActtt                                                                                                                                                                                           >  1:454192‑M2/192‑300 (MQ=255)                                                                                                               cccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCt                                                                                                                                                                                        <  2:778098‑M2/113‑1 (MQ=255)                                                                                                               cacgtggtctgggaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCt                                                                                                                                                                                        <  2:590744‑M2/113‑1 (MQ=255)                                                                                                                ccggtgtctggcaccctggtaatcgcccgctgattggggtgaccggccgcgagtcgcagcgttttgtctccgtggaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgttcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTg                                                                                                                                                                                       <  2:964747‑M2/114‑1 (MQ=255)                                                                                                                 cgtggtctggaaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGt                                                                                                                                                                                      <  1:409833‑M2/115‑1 (MQ=255)                                                                                                                                    attcgcccgctgatgggggttaccggccgtggtgcgcagtgttttgcttgctgggaggtgaactgtggcagtggggaaggcctgagcctggaacctgggcagcgggcgcaccggcgttggcaggcgaaccggctcagttgaggtcgtcgtctgcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAtgtt                                                                                                                                                                   <  1:466194‑M2/134‑1 (MQ=255)                                                                                                                                     atcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTTGGTCGGGAAGACAGCCAGCTGTGTGAGCAGCAGGCGTTTTGCACGGTCGTCTATCGTACTGTCGGTTCGGTTGAcgtaccacttggttgtaaccatttttggtaatgttt                                                                                                                                                                  >  2:466173‑M2/167‑284 (MQ=255)                                                                                                                                          ccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGtt                                                                                                                                                             <  1:89046‑M2/140‑1 (MQ=255)                                                                                                                                              tgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAACCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGGTACACTTGTGGGTAATGTTGTGgtttttt                                                                                                                                                         >  2:515823‑M2/158‑300 (MQ=255)                                                                                                                                                     ggttgacggcgggggggtagaagcgtgttgtctgcgtcgaggcgaccagtggaggtggcggggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGAGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTAGTTTTTACTACAtt                                                                                                                                                  <  2:951931‑M2/151‑1 (MQ=255)                                                                                                                                                     ggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACAtt                                                                                                                                                  <  2:426961‑M2/151‑1 (MQ=255)                                                                                                                                                     ggggaccggccgcgggtggcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACAtt                                                                                                                                                  <  2:454172‑M2/151‑1 (MQ=255)                                                                                                                                                          ccggccgcgggtcgcagcgttttgtctgctgcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATccc                                                                                                                                             <  2:223660‑M2/156‑1 (MQ=255)                                                                                                                                                                  gagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTgaaaaa                                                                                                                                     >  1:319817‑M2/138‑300 (MQ=255)                                                                                                                                                                   ggtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAAcc                                                                                                                                    <  1:519880‑M2/165‑1 (MQ=255)                                                                                                                                                                      cgcatcgttttgtcgtcgtcgaggcgaccagtggaagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgaaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAACCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCgtg                                                                                                                                 <  1:515844‑M2/168‑1 (MQ=255)                                                                                                                                                                       tgtgctcttccgatctgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCgtgt                                                                                                                                <  1:698802‑M2/169‑1 (MQ=255)                                                                                                                                                                              tttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAg                                                                                                                         >  1:758367‑M2/126‑301 (MQ=255)                                                                                                                                                                                       gtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACAttatcccttgaaacccgtgtagatcggaagggcgtg                                                                                                                >  2:698780‑M2/117‑285 (MQ=255)                                                                                                                                                                                         cgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTa                                                                                                              >  2:284762‑M2/115‑301 (MQ=255)                                                                                                                                                                                                                aaggcctgagcctggaacctgggaggcgtgcgcacctgcgtttgcaggcgaaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCAGGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCaa                                                                                       <  1:284769‑M2/210‑1 (MQ=255)                                                                                                                                                                                                                           ctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCg                                                                            >  1:753254‑M2/81‑301 (MQ=255)                                                                                                                                                                                                                               aacctgggcagcttgcgaacatgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAAt                                                                        <  2:738957‑M2/225‑1 (MQ=255)                                                                                                                                                                                                                                acctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATc                                                                       >  1:612757‑M2/76‑301 (MQ=255)                                                                                                                                                                                                                                   ggggcagcgggcgaaccggcgtttgaaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAgt                                                                    <  2:319808‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                                      gaagggtgggaacttgcgtttgaaggggaaccggttcatttgaggtcgtcgtcgtcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTc                                                                 <  2:328372‑M2/232‑1 (MQ=255)                                                                                                                                                                                                                                                     gcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCCAACCTTGCACCttt                                                  >  2:236588‑M2/55‑301 (MQ=255)                                                                                                                                                                                                                                                          tgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCt                                             >  1:60085‑M2/50‑301 (MQ=255)                                                                                                                                                                                                                                                               gcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCCCGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTg                                        <  2:612735‑M2/257‑1 (MQ=255)                                                                                                                                                                                                                                                                     cggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGccc                                  >  2:404192‑M2/39‑301 (MQ=255)                                                                                                                                                                                                                                                                       cgacgctcttccgatctgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTc                                <  2:113598‑M2/265‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ccgagttcctcgttagggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCa                    <  2:169315‑M2/277‑1 (MQ=255)                                                                                                                                                                                                                                                                                        gtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTgccctgtttggcgccctcagatcggaagagcacac               >  1:113600‑M2/20‑284 (MQ=255)                                                                                                                                                                                                                                                                                          cctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGacctggcaagatcgg             >  1:169319‑M2/18‑294 (MQ=255)                                                                                                                                                                                                                                                                                               atcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAGCATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGTCATTGCGCAAGctt        >  2:668409‑M2/13‑300 (MQ=255)                                                                                                                                                                                                                                                                                                tcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCTCTCGGCGACATGGCATTGCGCAAGCTGt       >  2:696385‑M2/12‑301 (MQ=255)                                                                                                                                                                                                                                                                                                     taatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCCt  >  1:698625‑M2/7‑301 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       CGAAGATCTTCTGGGTGTAGGCGGCCGGGTCATCGTCGGCCTCTACCGCGCGGCTGACATAGGCGGCACCACTGGCCAGGCTCTGCGCCAGTTGCAGCTTGAGTTCGGGGTAGCCGCTGACGCCGAACGAGCGGCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGGTCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCCT  >  AE015451/1165104‑1165696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑