Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	1,164,572	830 bp→TCA	coding (4‑833/864 nt)	hexR ←	DNA‑binding transcriptional regulator

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	1164572	1165401	830	99 [0]	[0] 99	hexR	DNA‑binding transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1164571	0 (0.000)	99 (0.900) +TCA	83/594	0.2	100%	coding (834/864 nt)	hexR	DNA‑binding transcriptional regulator
	?	AE015451	1165402 =	0 (0.000)					coding (3/864 nt)	hexR	DNA‑binding transcriptional regulator

GGCGAAGATCTTCTGGGTGTAGGCGGCCGGGTCATCGTCGGCCTCTACCGCGCGGCTGACATAGGCGGCACCACTGGCCAGGCTCTGCGCCAGTTGCAGCTTGAGTTCGGGGTAGCCGCTGACGCCGAACGAGCGGCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCCTG  >  AE015451/1165102‑1165697                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                         gctgaagctcaagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcac                                                                                                                                                                                                                                                                                                          >  3:55334‑M2/301‑301 (MQ=255)    gaagctcaagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcacacc                                                                                                                                                                                                                                                                                                       >  3:615274‑M2/298‑300 (MQ=255)       gccaaggggcggcggccagaagggctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCgg                                                                                                                                                                                                                                                                                                    <  2:71670‑M2/7‑1 (MQ=255)        gctaagggcggctgccagaaggtcgaccccggtacggcacgggtgcaattgcaggacccagcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtgggctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGt                                                                                                                                                                                                                                                                                                   <  2:166790‑M2/8‑1 (MQ=255)           aagggcggctgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcaaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGtccc                                                                                                                                                                                                                                                                                                >  3:534249‑M2/291‑300 (MQ=255)                   gggagttcagacgtgtgctcttccgatctgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGgtgtg                                                                                                                                                                                                                                                                                        <  3:485287‑M2/19‑1 (MQ=255)                    tgccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGgtgtgt                                                                                                                                                                                                                                                                                       >  4:48554‑M2/282‑301 (MQ=255)                     gccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatccCACGCGgtccatggggttgtt                                                                                                                                                                                                                                                                                      >  2:483400‑M2/281‑295 (MQ=255)                     gccagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTc                                                                                                                                                                                                                                                                                      >  4:324423‑M2/281‑301 (MQ=255)                       cagaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCt                                                                                                                                                                                                                                                                                    >  4:511779‑M2/279‑301 (MQ=255)                        agaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCtt                                                                                                                                                                                                                                                                                   <  3:197430‑M2/24‑1 (MQ=255)                        agaaggtctaccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcacagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgttggcaggcgcaccggcacagttgagggctgcgtccccgatcgggtaatcccacgcggtaccgggggttttcctt                                                                                                                                                                                                                                                                                   >  2:415723‑M2/278‑285 (MQ=255)                                taccccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaagcaCACGCGGTCACTGGGTGTGTCCTTGGGTgggg                                                                                                                                                                                                                                                                           >  1:32350‑M2/270‑301 (MQ=255)                                   cccggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTTTCCTTGGGTCGGGAAg                                                                                                                                                                                                                                                                        >  1:245012‑M2/267‑301 (MQ=255)                                      ggtacgccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACa                                                                                                                                                                                                                                                                     >  4:70863‑M2/264‑301 (MQ=255)                                           tcccgatcggaaagtgcaggccccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCgg                                                                                                                                                                                                                                                                <  2:305892‑M2/43‑1 (MQ=255)                                           gccacgggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaTCCGTGGTCCATGGGTGTGGCCTTGGgtcgggaaggccaccgg                                                                                                                                                                                                                                                                >  2:547833‑M2/259‑293 (MQ=255)                                                gggtgcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcacacgcggtccatgggtgtgagatcggaagagcgtcgtgtagggaaagg                                                                                                                                                                                                                                                           >  1:485273‑M2/254‑272 (MQ=255)                                                gggtgcaattgcaggacccggcctggaagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCtgtg                                                                                                                                                                                                                                                           >  1:351381‑M2/254‑301 (MQ=255)                                                    gcaattgcaggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGaagacagccggagatcggaa                                                                                                                                                                                                                                                       >  4:305954‑M2/250‑292 (MQ=255)                                                        gtgcaggagccggcctggaagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGagcagc                                                                                                                                                                                                                                                   <  2:431008‑M2/56‑1 (MQ=255)                                                            aggacccggcctggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCaggg                                                                                                                                                                                                                                               >  3:464120‑M2/242‑300 (MQ=255)                                                                 ccgggctgggagcgggggctgggcatagataccggtgacagcgccgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGTGAAGACAGCCGGCTGTGTGAGCAGCAGGCttttt                                                                                                                                                                                                                                          <  2:242491‑M2/65‑1 (MQ=255)                                                                        ggcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGACGCCAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCCcggg                                                                                                                                                                                                                                   >  2:172184‑M2/230‑300 (MQ=255)                                                                        ggcagcgcgagctgcgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagtcgaggtcgtcgtcctcgaccgggtaatcaCACGCGGTCCACGGGTCTGTCcttgggtcgggcagacagccggctgtgttcgcatgaggctctttgcccggt                                                                                                                                                                                                                                   >  2:166646‑M2/230‑278 (MQ=255)                                                                         gcagcgcgagctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGg‑tc                                                                                                                                                                                                                                 <  4:166677‑M2/73‑1 (MQ=255)                                                                                 ggctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCg                                                                                                                                                                                                                         <  4:310664‑M2/81‑1 (MQ=255)                                                                                 agctgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCg                                                                                                                                                                                                                         <  4:461469‑M2/81‑1 (MQ=255)                                                                                    tgtgcatcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcggttaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCCTCTATCGTAc                                                                                                                                                                                                                      >  2:645129‑M2/218‑301 (MQ=255)                                                                                         gacgataccggtgacagggacgaaaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggcagcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCg                                                                                                                                                                                                                 <  2:304609‑M2/89‑1 (MQ=255)                                                                                         atcgataccggtgacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCg                                                                                                                                                                                                                 >  3:74250‑M2/213‑301 (MQ=255)                                                                                                     gacagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCGTGGGTCGGGAAGACAGCCGGCTGTGCGAGCAGCAGGCTTTTTGCCCGGTTCGT‑TATCGTACTTTCGGTGCGgttgcag                                                                                                                                                                                                     >  2:372381‑M2/201‑298 (MQ=255)                                                                                                       cagcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTa                                                                                                                                                                                                   <  1:4214‑M2/103‑1 (MQ=255)                                                                                                        agcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCCTTTTGCACGG‑TCGTCGTTCGTACTTTcggtgcggtgtacgtgt                                                                                                                                                                                                  >  2:612773‑M2/198‑293 (MQ=255)                                                                                                         gcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTAcc                                                                                                                                                                                                 <  3:351390‑M2/105‑1 (MQ=255)                                                                                                         gcgacgacaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcaaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTAcc                                                                                                                                                                                                 <  1:534235‑M2/105‑1 (MQ=255)                                                                                                               acaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgatcagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGCGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTTCGGTTGACGTACCACTTGt                                                                                                                                                                                           >  2:327940‑M2/191‑301 (MQ=255)                                                                                                               acaccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGt                                                                                                                                                                                           <  1:74248‑M2/111‑1 (MQ=255)                                                                                                                caccgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTc                                                                                                                                                                                          <  1:615259‑M2/112‑1 (MQ=255)                                                                                                                 ccctgggtctggcaccctggcaatcgcccgctggtgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCt                                                                                                                                                                                         <  2:324359‑M2/113‑1 (MQ=255)                                                                                                                 accgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTTCGGTTGTCGTACCACTTTTCt                                                                                                                                                                                         >  2:381612‑M2/189‑301 (MQ=255)                                                                                                                 accgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTTCGGTTGACGTACCACTTGTCt                                                                                                                                                                                         >  1:227748‑M2/189‑301 (MQ=255)                                                                                                                 accgtggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTCAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCt                                                                                                                                                                                         >  4:180801‑M2/189‑301 (MQ=255)                                                                                                                     tggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTaa                                                                                                                                                                                     <  4:484104‑M2/117‑1 (MQ=255)                                                                                                                      ggtctggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAac                                                                                                                                                                                    <  3:32351‑M2/118‑1 (MQ=255)                                                                                                                          tggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACtt                                                                                                                                                                                >  4:734752‑M2/180‑301 (MQ=255)                                                                                                                          tggcaccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACtt                                                                                                                                                                                >  1:522225‑M2/180‑301 (MQ=255)                                                                                                                             caccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTtgtg                                                                                                                                                                             >  3:275796‑M2/177‑301 (MQ=255)                                                                                                                               ccctggcaatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTtgtgtg                                                                                                                                                                           <  1:464108‑M2/127‑1 (MQ=255)                                                                                                                                      aatcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAtgtt                                                                                                                                                                    <  4:483517‑M2/134‑1 (MQ=255)                                                                                                                                        tcgcccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAAtgttgt                                                                                                                                                                  >  4:396940‑M2/166‑301 (MQ=255)                                                                                                                                          ccccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTgg                                                                                                                                                                <  3:227751‑M2/138‑1 (MQ=255)                                                                                                                                           cccgctgatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGt                                                                                                                                                               <  1:136297‑M2/139‑1 (MQ=255)                                                                                                                                            cccttgagtggggggaccgcccgcgagtggcagcgttttgtctgcgccgaggagaccagtggcagtgtcgaaggcctgagcctggcaccggggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGtt                                                                                                                                                              <  2:48542‑M2/140‑1 (MQ=255)                                                                                                                                                ggatgggggtgaccggccgcgagtcgcagcgttgtgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTta                                                                                                                                                          <  1:55333‑M2/144‑1 (MQ=255)                                                                                                                                                 gatgggggtgaccggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTtac                                                                                                                                                         <  4:172215‑M2/145‑1 (MQ=255)                                                                                                                                                             tggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCt                                                                                                                                             <  4:147139‑M2/157‑1 (MQ=255)                                                                                                                                                             tgctcttccgatctagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCt                                                                                                                                             <  4:754963‑M2/157‑1 (MQ=255)                                                                                                                                                              ggccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGGGTAATGTTGTGGTTTTTACTACATTATCCCtt                                                                                                                                            >  2:147109‑M2/144‑301 (MQ=255)                                                                                                                                                                ccgcgagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGa                                                                                                                                          >  3:98236‑M2/142‑301 (MQ=255)                                                                                                                                                                    gagtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTgaaaaa                                                                                                                                      >  2:233259‑M2/138‑300 (MQ=255)                                                                                                                                                                      gtcgcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCg                                                                                                                                    <  3:245016‑M2/166‑1 (MQ=255)                                                                                                                                                                         gcagcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCgtgt                                                                                                                                 <  1:341850‑M2/169‑1 (MQ=255)                                                                                                                                                                           agcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGAACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTCATGTTGTTGTTTTtactacattttccctagatcggaagggcg                                                                                                                               >  2:754747‑M2/131‑287 (MQ=255)                                                                                                                                                                            gcgttttgtctgcgtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCACTTGAAAACCGtgtgtg                                                                                                                              <  4:645299‑M2/172‑1 (MQ=255)                                                                                                                                                                                     gggagttcagacgtgtgctcttccgatctaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTa                                                                                                                     <  3:426708‑M2/181‑1 (MQ=255)                                                                                                                                                                                         gtcgaggcgaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTcc                                                                                                                 >  3:40081‑M2/117‑301 (MQ=255)                                                                                                                                                                                            gaggcgaccaggggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAg                                                                                                              <  4:547971‑M2/188‑1 (MQ=255)                                                                                                                                                                                              atctaccagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGAc                                                                                                            <  4:46681‑M2/190‑1 (MQ=255)                                                                                                                                                                                               gcgacaagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGAcc                                                                                                           <  1:98234‑M2/191‑1 (MQ=255)                                                                                                                                                                                                  accagtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCtagacagat                                                                                                        >  2:46669‑M2/108‑297 (MQ=255)                                                                                                                                                                                                     ggtggcagtggcggaggcctgggcctggcaccgtggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACttt                                                                                                     <  2:665092‑M2/197‑1 (MQ=255)                                                                                                                                                                                                     agtggcagtggcgaaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACttt                                                                                                     >  4:665268‑M2/105‑301 (MQ=255)                                                                                                                                                                                                       cagacgtgtgctcttccgatctctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAg                                                                                                   <  3:541803‑M2/199‑1 (MQ=255)                                                                                                                                                                                                                ctctttccctacacgacgctcttccgatcttgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAAcc                                                                                          <  1:365306‑M2/208‑1 (MQ=255)                                                                                                                                                                                                                  aaggcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTatcccttgaaaaccgtgtgtgaaagcggtaagatcggaagagcgtcgtgtagggaaaga                                                                                        >  1:426697‑M2/92‑272 (MQ=255)                                                                                                                                                                                                                    ggccggggcctggcgcctgggcagcttccgaacctgggtttgcaggcgaaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAAca                                                                                      <  2:396857‑M2/212‑1 (MQ=255)                                                                                                                                                                                                                     gcctgagcctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAAcat                                                                                     >  4:659983‑M2/89‑301 (MQ=255)                                                                                                                                                                                                                           cgctcttccgatcgcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcttcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGc                                                                               <  2:9169‑M2/219‑1 (MQ=255)                                                                                                                                                                                                                             ctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGcggtattcctagaccaactttagagatcggaagagcgtcgggtag                                                                             >  1:541789‑M2/81‑279 (MQ=255)                                                                                                                                                                                                                             ctggcacctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCg                                                                             <  4:415807‑M2/221‑1 (MQ=255)                                                                                                                                                                                                                                 ggcctgggcagcgtgggcacctgcggttgcaggggcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAAt                                                                         <  2:511653‑M2/225‑1 (MQ=255)                                                                                                                                                                                                                                  acctgggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATc                                                                        <  4:381688‑M2/226‑1 (MQ=255)                                                                                                                                                                                                                                       ggcagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAgtgt                                                                   >  3:206907‑M2/71‑301 (MQ=255)                                                                                                                                                                                                                                         cagcgtgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGaaccaacatcatggcagatcggaagagca                                                                 >  4:9169‑M2/69‑287 (MQ=255)                                                                                                                                                                                                                                              tgcgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGtattcctagaccaactttaggaaagaaccagatcggaagagcacacgtctgaactccag                                                            >  3:365317‑M2/64‑272 (MQ=255)                                                                                                                                                                                                                                               gggcacctgcgtttgcaggggcaccggtcaagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCtt                                                           <  2:70848‑M2/239‑1 (MQ=255)                                                                                                                                                                                                                                                cgcacctgcgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTg                                                          <  4:612929‑M2/240‑1 (MQ=255)                                                                                                                                                                                                                                                        cgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACctttt                                                  >  1:444602‑M2/54‑300 (MQ=255)                                                                                                                                                                                                                                                        cgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTg                                                  <  3:522239‑M2/248‑1 (MQ=255)                                                                                                                                                                                                                                                        cgtttgcaggcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTg                                                  <  4:328004‑M2/248‑1 (MQ=255)                                                                                                                                                                                                                                                                 gcgcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTg                                         <  4:233298‑M2/257‑1 (MQ=255)                                                                                                                                                                                                                                                                  ggcaccggctcagttgaggtcgtcgtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTgg                                        <  1:275791‑M2/258‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gtcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCTTtgcc                >  2:235110‑M2/20‑300 (MQ=255)                                                                                                                                                                                                                                                                                           tcctcgatcgggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTgcgc               >  4:312831‑M2/19‑301 (MQ=255)                                                                                                                                                                                                                                                                                                     ggtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTc     >  2:316939‑M2/9‑301 (MQ=255)                                                                                                                                                                                                                                                                                                      gtaatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTcc    <  1:40080‑M2/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        aatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTGGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCCCCCTCGGCGACCTGGCATTTCGCCACCTGTTTCctc  >  2:524508‑M2/6‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        aatcaCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCctt  >  3:158238‑M2/6‑300 (MQ=255)                                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                         GGCGAAGATCTTCTGGGTGTAGGCGGCCGGGTCATCGTCGGCCTCTACCGCGCGGCTGACATAGGCGGCACCACTGGCCAGGCTCTGCGCCAGTTGCAGCTTGAGTTCGGGGTAGCCGCTGACGCCGAACGAGCGGCAGAAGCGGTTGACGGTCGGTTCGCTGACCTTGGCCGCCTGGGCCAGCGCAGCGATGCTGAAACGGGTGGCTTGTTGCGGGTTGAGCAGGATGACTTCGGCGACTTTGCGTTCGGCCTTGTTCAGCTCGTCGAGGCGGCCCTGGATCTGTTCCAGGAGGTTTCGCACGCGGTCCATGGGTGTGTCCTTGGGTCGGGAAGACAGCCGGCTGTGTGAGCAGCAGGCTTTTTGCACGG‑TCGTCTATCGTACTGTCGGTGCGGTTGACGTACCACTTGTCTGTAACACTTGTGTGTAATGTTGTGGTTTTTACTACATTATCCCTTGAAAACCGTGTGTGAAAGCGGTATTCCTAGACCAACTTTAGGAAAGAACCAACATCATGGCCGCAATCAGTGTCGAACCTTGCACCTTTGCCCTGTTTGGCGCCCTCGGCGACCTGGCATTGCGCAAGCTGTTTCCTG  >  AE015451/1165102‑1165697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑