New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	550100 =	NA (NA)	5 (0.050)	5/574	7.7	4.5%	coding (775/1194 nt)	tufB	Elongation factor Tu‑B
	?	AE015451	= 550096	112 (1.060)					coding (771/1194 nt)	tufB	Elongation factor Tu‑B
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAggtggtggtggtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/550398‑550100 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATCGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCGATGATGATCGGAGTGTCGTCGCCTGGGAAGTCGTAGGTGGACAGCAGGTCGCGAACT  <  AE015451/550096‑549784                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      cCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGG                                                                                                                                                                                                                                                                                                                         <  2:733011/300‑1   cCCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTG                                                                                                                                                                                                                                                                                                                       <  1:13423/300‑1     CTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGg                                                                                                                                                                                                                                                                                                                     >  2:382740/1‑300        GCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGg                                                                                                                                                                                                                                                                                                                  >  3:601495/1‑300         gacgtgtgctcttccgatcTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTATCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTG                                                                                                                                                                                                                                                                                                                 <  3:512652/282‑1         CATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTG                                                                                                                                                                                                                                                                                                                 <  1:507537/301‑1         CATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTG                                                                                                                                                                                                                                                                                                                 >  4:195399/1‑301           TTACAATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGT                                                                                                                                                                                                                                                                                                               <  2:372229/301‑1            TACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTG                                                                                                                                                                                                                                                                                                              <  1:315808/301‑1                ATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGGGTCAC                                                                                                                                                                                                                                                                                                          >  4:6090/1‑301                        GCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGTCACGCAGACCA                                                                                                                                                                                                                                                                                                  >  4:526387/1‑301                             CCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTATCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGGGGTGagatcggaagagcgtcgtgg                                                                                                                                                                                                                                                                                             >  1:512639/1‑281                                                                                                 AACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCG                                                                                                                                                                                                                         >  1:127847/1‑301                                                                                                            cCGGCCGACTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAAC                                                                                                                                                                                                              <  2:6089/300‑1                                                                                                                                                          TTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAGGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATCGGCATCAGGAACGGCTGGTCGATGGCACGAACTG                                                                                                                                                                <  3:127850/301‑1                                                                                                                                                                                                                                                                                                            GGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATCGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCGATGATGATCGGAGTGTCGTCGCCTGGGAAGTCGTAGGTGGACAGC              <  4:204985/301‑1                                                                                                                                                                                                                                                                                                                 gGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATCGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCGATGATGATCGGAGTGTCGTCGCCTGGGAAGTCGTAGGTGGACAGCAGGTC         <  3:497323/300‑1                                                                                                                                                                                                                                                                                                                    gGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATCGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCGATGATGATCGGAGTGTCGTCGCCTGGGAAGTCGTAGGTGGACAGCAGGTCGCG      <  4:614350/300‑1                                                                                                                                                                                                                                                                                                                       TGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATCGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCGATGATGATCGGAGTGTCGTCGCCTGGGAAGTCGTAGGTGGACAGCAGGTCGCGAAC   >  1:494753/1‑301                                                                                                                                                                                                                                                                                                                        GTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATCGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTTTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCGAAGATGATCGGAGTGTCGTCGCCTGGGAAGTCGTAGGTGGACAGCAGGTCGCGAACc  >  4:764861/1‑300                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      TCACCTGGCATTACCATTTCAACGCCTTCCGGCAGTTCGCAGTTACCGGTCACGTCAGTGGTACGGAAGTAGAACTGTGGACGGTAGCCTTTGAAGAACGGAGTGTGACGGCCGCCTTCTTCCTTCGACAGGACGTAGACTTCTGCGGTGAACTTGGTGTGCGGCTTGACCGAACCTGGCTTGACCAGAACCTGGCCACGCTCAACGTCGTCACGCTTGGTACCACGCAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCCAGCAGCTTGCGGAACATCTCAACACCGGTGCAggtggtggtggtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/550398‑550100 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGGTGGTGGTGTCACGCAGACCAACGATTTCCAGCGGATCCTGAACGCGAACGATGCCACGCTCGATACGACCGGTAACAACGGTACCACGACCCGAGATCGAGAACACGTCTTCGATCGGCATCAGGAACGGCTGGTCGATGGCACGAACTGGCTCAGGGATGTAGGCATCCAGAGTTTCTACCAGCTTCTTGACAGCGGTAGTGCCCATTTCGTTGTCGTCTTTGCCTTCCAGGGCCATACGAGCGGAACCGATGATGATCGGAGTGTCGTCGCCTGGGAAGTCGTAGGTGGACAGCAGGTCGCGAACT  <  AE015451/550096‑549784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.