Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	5,342,745	1681 bp→GCGGCCGC		[pgi‑II]	[pgi‑II]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	5342745	5344425	1681	50 [0]	[0] 50	[pgi‑II]	[pgi‑II]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5342744	0 (0.000)	49 (0.610) +GCGGCCGC	47/584	0.6	100%	intergenic (+190/‑17)	panC/pgi‑II	pantothenate synthetase/glucose‑6‑phosphate isomerase
	?	AE015451	5344426 =	0 (0.000)					intergenic (+1/‑177)	pgi‑II/acsA‑II	glucose‑6‑phosphate isomerase/acetyl‑CoA synthetase

AGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGGGAACCCCGCGCAATGGCGTATTACCGTACACCCCACGATGTGACGGCCCTGCCCGCCTGGCAGGCGCTTCAGAAACACCGCGACGCCATGCAAAGCTTCAGCATGCGCGAAGCCTTCGCCGCCGATGCCAAGCGCTTCGACCAGTTTTCCCTCAGCGCTTGCGGCCTGTTCCTCGACTACTCGAAAAACCTGATCACCGAGCAAAGCCGCGATCTGCTGGTCAATCTGGCCAATGAAGTGGGCCTGCAGGACGC  >  AE015451/5342452‑5343002                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                   aGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgc                                                                                                                                                                                                                                                            <  1:587710‑M1/301‑9 (MQ=255)          tGATCGAGACCTGGTGGTGCTTCGGGCGGCTTATCGGGGTAGACA‑GCGGTTGATCGACAACCTTTACCTGCATTTGGAGGCGAAGACCGCAGAACGGTCTTGCGGTGCCTTGCAGCCGAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGTGCGGGCTTGCTTGCAATGCGCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTCGCCTTTGCCTATAACGGTGCCAGCCTGAACCCGGCAACGACCGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagctt                                                                                                                                                                                                                                                   <  2:373684‑M1/301‑18 (MQ=255)           gATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCCCCCCGCCCCgcggccgccccgagcttt                                                                                                                                                                                                                                                  >  2:39371‑M1/1‑283 (MQ=255)            aTCGAGACCTGGTGGTGATTGTGGCGGCTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgt                                                                                                                                                                                                                                                 <  1:25091‑M1/301‑20 (MQ=255)            aTCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgt                                                                                                                                                                                                                                                 <  1:156448‑M1/301‑20 (MQ=255)              ggggtactggtggTGATTGCGGCTGCTTACCTGGGTA‑CCAGCCGCTTGCTCGACAACCTCTACCTGCATTTGGAAGGGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgc                                                                                                                                                                                                                                               <  2:670095‑M1/295‑22 (MQ=255)                      ggtggtGATTGCGGCGGCTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctct                                                                                                                                                                                                                                       <  1:39373‑M1/301‑30 (MQ=255)                                   ggttcttacCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacc                                                                                                                                                                                                                          <  2:492729‑M1/297‑43 (MQ=255)                                    cggcTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTCCCCTGTCCAAGCCCTCACCACGCACCccggccccgccgagcttgtgcccgtctcttcgccggtaaaccc                                                                                                                                                                                                                         >  2:373589‑M1/1‑258 (MQ=255)                                     ggcTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccg                                                                                                                                                                                                                        <  1:64129‑M1/301‑45 (MQ=255)                                     ggcTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggccccctcgcgggtaaccccc                                                                                                                                                                                                                        >  1:695345‑M1/1‑257 (MQ=255)                                        ttACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGACAGCCTGAACCCGGCAACGACTGCCGTGTCCACGCCCTCACCACGCACCgcggccgctcccagcttgtgacggccccttcgcgggtaacaccgccc                                                                                                                                                                                                                     >  2:349007‑M1/1‑254 (MQ=255)                                        ttACCTGGGTA‑AAACACGGTTGATTGACAACCTCAACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcct                                                                                                                                                                                                                     <  1:502385‑M1/301‑48 (MQ=255)                                         tACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaaccagatcg                                                                                                                                                                                                                    >  1:492750‑M1/1‑253 (MQ=255)                                            cTGGGTA‑ACACGCGGTGGTTCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctacca                                                                                                                                                                                                                 <  1:373601‑M1/301‑52 (MQ=255)                                                 tA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaa                                                                                                                                                                                                            >  1:63545‑M1/1‑245 (MQ=255)                                                    acacGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTTGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacg                                                                                                                                                                                                          <  2:63543‑M1/301‑59 (MQ=255)                                                                ccgagctcctctacctGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgta                                                                                                                                                                                              <  2:385269‑M1/294‑71 (MQ=255)                                                                   aCAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTTTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggg                                                                                                                                                                                           >  2:831319‑M1/1‑228 (MQ=255)                                                                       ccTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTTTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaagatcgg                                                                                                                                                                                       >  1:385281‑M1/1‑224 (MQ=255)                                                                       ccTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcggtaccccgtaggagcgg                                                                                                                                                                                       >  2:37923‑M1/1‑224 (MQ=255)                                                                           cacCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggttt                                                                                                                                                                                   <  1:831342‑M1/300‑82 (MQ=255)                                                                             caggcatTTGGAAGAGAAGACCGAAAAACGGTCTTGGGCTGCCTTGCAGCCCAT‑ACGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGAACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttac                                                                                                                                                                                 <  1:175805‑M1/298‑84 (MQ=255)                                                                              cTGCATTTGGAAGAGAAGACCGAATAACGGTCTTGGGCTGCCGTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTGTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacc                                                                                                                                                                                <  1:349018‑M1/301‑85 (MQ=255)                                                                               ggaatttGGAAGAGAAGACCGAATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttaccc                                                                                                                                                                               <  2:316721‑M1/298‑86 (MQ=255)                                                                                 cATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggccccttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgc                                                                                                                                                                             >  1:141511‑M1/1‑214 (MQ=255)                                                                                      ggAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgccccgagcttgtgccggccccttcgcgggtaaacccgcctaccaggcaacgcgttaccccggaggaccgggtttacccgccaaga                                                                                                                                                                        >  2:894110‑M1/1‑209 (MQ=255)                                                                                        aagagaagaCCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCAATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaaccagttacctcgtaggagcgggtttacccgcgaagagg                                                                                                                                                                      >  1:158384‑M1/1‑207 (MQ=255)                                                                                          cttccagttccatatcggtctTGGGCTGCCTGGCAGCCCATCTCG‑GGGCAAGCCCGCTCTTTCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggcc                                                                                                                                                                    <  2:618333‑M1/291‑97 (MQ=255)                                                                                                 ccGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttaactcgtacgagcgggtttacccgcgaagaggacggcccac                                                                                                                                                             >  2:17323‑M1/1‑198 (MQ=255)                                                                                                   ccaTAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggt                                                                                                                                                           <  1:894133‑M1/300‑106 (MQ=255)                                                                                                    cATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgccggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccagatcggaag                                                                                                                                                          >  1:618355‑M1/1‑195 (MQ=255)                                                                                                    aataccgtccttgggctgcCTTGCAGCCCAT‑TCGCGGCCAGGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggtt                                                                                                                                                          <  1:17325‑M1/292‑107 (MQ=255)                                                                                                         gggctttgggccGCCTTGCAGCCCAT‑TGGCGACCAAGCCCGATCCTGGACGTTTACCGGTAGGGGCGGGCTTGCTTGCAATGCCCCCACAATCCCCCTGCCATCCCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaaca                                                                                                                                                     <  2:695323‑M1/296‑112 (MQ=255)                                                                                                                  cctGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctccca                                                                                                                                            <  1:37925‑M1/300‑121 (MQ=255)                                                                                                                                 aT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgccttacctcgtaggagccggtttacccgcgaagaagccggcccaggttgaacaccggtcccagctacactgtcttat                                                                                                                             >  1:266579‑M1/1‑166 (MQ=255)                                                                                                                                                        gCACGTTTACCTGTAGGGACGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatc                                                                                                       <  1:774574‑M1/301‑158 (MQ=255)                                                                                                                                                               tACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctaccctgttctatcgaataaccgttgcagtccaacgcccctt                                                                                                >  2:37065‑M1/1‑137 (MQ=255)                                                                                                                                                                    gTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacaatgtcttatcgaatagccgttgcagtccatcgccccttaaaaa                                                                                           >  1:205175‑M1/1‑132 (MQ=255)                                                                                                                                                                            gggcTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggatcgggtttacccgcgcagaggccggccccggttgaacaccgctcccagctacaatgtcttatcgactatccgttgccgtccttagatcgtcaggcccttgttgt                                                                                   >  2:472770‑M1/1‑124 (MQ=255)                                                                                                                                                                                  gcttgcAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatcgccccttacaagagcaggaccacccg                                                                             <  2:141508‑M1/301‑184 (MQ=255)                                                                                                                                                                                        aaTGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatcgccccttacaagagcaggaccacccgccatgt                                                                       <  1:37067‑M1/301‑190 (MQ=255)                                                                                                                                                                                                ccacatcccCCTGCCATACCCATTCCCAGAACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatcgccccttacaagagcaggaccacccgccatgttcgatatc                                                               <  2:205170‑M1/297‑198 (MQ=255)                                                                                                                                                                                                 aaaaGCCCCCTGCCATACCCCTTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatcgccccttacaagagcaggaccacccgccatgttcgatatcc                                                              <  2:45084‑M1/301‑199 (MQ=255)                                                                                                                                                                                                            gCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggccccttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggttgacccgcgaagaggccggcccaggttgcacagcgcccccagctacactctcttatcgaatggccgttgcaggccatcgccccttacaagagcagaacaacccgcaatgttcgatatccgaaaatacccc                                                   >  2:736622‑M1/1‑92 (MQ=255)                                                                                                                                                                                                                 ccccatacccaaaaagtggcctttgcctataATGGGGCAAGCCTGAACCCGGAAACGACAGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatcgccccttacaagagcaggaccacccgccatgttcgatatccgcaaatacccccaggc                                              <  1:902862‑M1/286‑215 (MQ=255)                                                                                                                                                                                                                     aTTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatcgccccttacaagagcaggaccacccgccatgttcgatctccgcaaataccccccgcccctg                                          >  2:181532‑M1/1‑83 (MQ=255)                                                                                                                                                                                                                                gTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggccccttcgcgggtaaacccgcctaccaggcaacgcgttacctcgcaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttaacgaatacccgttgcagtccatcgcccctcacaagagcaggacccccctcccacttcgatccccgcaaacaccccacgtccctggccgccccacc                               >  2:247679‑M1/1‑72 (MQ=255)                                                                                                                                                                                                                                                           cTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatcgccccttacaagagcaggaccacccgccatgttcgatatccgcaaatacccccaggccctggccgtcagccagtccgccgcccccacccccgaagacca    >  1:859090‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                                                             aaacCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCgcggccgctccgagcttgtgccggcctcttcgcgggtaaacccgcctaccaggcaacgcgttacctcgtaggagcgggtttacccgcgaagaggccggcccaggttgaacaccgctcccagctacactgtcttatcgaatagccgttgcagtccatcgccccttacaagagcaggaccacccgccatgttcgatatccgcaaatacccccaggccctggccgtcagccagtccgccgccctcacccccgaagactacc  <  1:181536‑M1/300‑259 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                   AGTGATCAATGATCGAGACCTGGTGGTGATTGCGGCGGCTTACCTGGGTA‑ACACGCGGTTGATCGACAACCTCTACCTGCATTTGGAAGAGAAGACCGCATAACGGTCTTGGGCTGCCTTGCAGCCCAT‑TCGCGGGCAAGCCCGCTCCTGCACGTTTACCTGTAGGGGCGGGCTTGCTTGCAATGCCCCCAAAATCCCCCTGCCATACCCATTCCCAGCACGTGGCCTTTGCCTATAATGGTGCCAGCCTGAACCCGGCAACGACTGCCGTGTCCAAGCCCTCACCACGCACCAAGGGAACCCCGCGCAATGGCGTATTACCGTACACCCCACGATGTGACGGCCCTGCCCGCCTGGCAGGCGCTTCAGAAACACCGCGACGCCATGCAAAGCTTCAGCATGCGCGAAGCCTTCGCCGCCGATGCCAAGCGCTTCGACCAGTTTTCCCTCAGCGCTTGCGGCCTGTTCCTCGACTACTCGAAAAACCTGATCACCGAGCAAAGCCGCGATCTGCTGGTCAATCTGGCCAATGAAGTGGGCCTGCAGGACGC  >  AE015451/5342452‑5343002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑