Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AE015451	5,342,745	1681 bp→GCGGCCGC		[pgi‑II]	[pgi‑II]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AE015451	5342745	5344425	1681	50 [0]	[0] 50	[pgi‑II]	[pgi‑II]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5342744	0 (0.000)	49 (0.610) +GCGGCCGC	47/584	0.6	100%	intergenic (+190/‑17)	panC/pgi‑II	pantothenate synthetase/glucose‑6‑phosphate isomerase
	?	AE015451	5344426 =	0 (0.000)					intergenic (+1/‑177)	pgi‑II/acsA‑II	glucose‑6‑phosphate isomerase/acetyl‑CoA synthetase

CGAAAAGCTGGCCCCGCACAAGGTGATCCCGGGTAACCGCCCGAGCAACACGTTGGTGGTCGAGCGCATCAGCCCGCGCCGGCTGGGTGCGCTGGTGGCCATGTACGAGCACAAGGTGTTCGTACAGAGCGTTATCTGGGGTATCAACGCCTTCGATCAGTGGGGCGTCGAGCTGGGCAAGGAGCTGGGCAAGGGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGACCCAGACACCTTCTGG  >  AE015451/5344134‑5344719                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                      tgatcgagacctggtggtgcttcgggcggcttatcggggtagacagcggttgatcgacaacctttacctgcatttggaggcgaagaccgcagaacggtcttgcggtgccttgcagccgattcgcgggcaagcccgctcctgcacgtttacctgtaggtgcgggcttgcttgcaatgcgcccaaaatccccctgccatacccattcccagcacgtcgcctttgcctataacggtgccagcctgaacccggcaacgaccgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCtt                                                                                                                                                                                                                                                                                               <  2:373684‑M2/9‑1 (MQ=255)  gatcgagacctggtggtgattgcggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctccccccgccccgcggccgcCCCGAGcttt                                                                                                                                                                                                                                                                                              >  2:39371‑M2/292‑300 (MQ=255)   atcgagacctggtggtgattgtggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTtgt                                                                                                                                                                                                                                                                                             <  1:25091‑M2/11‑1 (MQ=255)   atcgagacctggtggtgattgcggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTtgt                                                                                                                                                                                                                                                                                             <  1:156448‑M2/11‑1 (MQ=255)     ggggtactggtggtgattgcggctgcttacctgggtaccagccgcttgctcgacaacctctacctgcatttggaagggaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGc                                                                                                                                                                                                                                                                                           <  2:670095‑M2/13‑1 (MQ=255)             ggtggtgattgcggcggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCctct                                                                                                                                                                                                                                                                                   <  1:39373‑M2/21‑1 (MQ=255)                          ggttcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAAcc                                                                                                                                                                                                                                                                      <  2:492729‑M2/34‑1 (MQ=255)                           cggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactcccctgtccaagccctcaccacgcaccccggccccGCCGAGCTTGTGCCCGTCTCTTCGCCGGTAAAccc                                                                                                                                                                                                                                                                     >  2:373589‑M2/267‑301 (MQ=255)                            ggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCg                                                                                                                                                                                                                                                                    <  1:64129‑M2/36‑1 (MQ=255)                            ggcttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCCTCGCGGGTAaccccc                                                                                                                                                                                                                                                                    >  1:695345‑M2/266‑300 (MQ=255)                               ttacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgacagcctgaacccggcaacgactgccgtgtccacgccctcaccacgcaccgcggccgcTCCCAGCTTGTGACGGCCCCTtcgcgggtaacaccgccc                                                                                                                                                                                                                                                                 >  2:349007‑M2/263‑293 (MQ=255)                               ttacctgggtaaaacacggttgattgacaacctcaacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCt                                                                                                                                                                                                                                                                 <  1:502385‑M2/39‑1 (MQ=255)                                tacctgggtaacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGggtaaaccagatcg                                                                                                                                                                                                                                                                >  1:492750‑M2/262‑295 (MQ=255)                                   ctgggtaacacgcggtggttcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCa                                                                                                                                                                                                                                                             <  1:373601‑M2/43‑1 (MQ=255)                                        taacacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCaa                                                                                                                                                                                                                                                        >  1:63545‑M2/254‑301 (MQ=255)                                          acacgcggttgatcgacaacctctacctgcatttggaagagaagaccgcataacggtctttggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAAcg                                                                                                                                                                                                                                                      <  2:63543‑M2/50‑1 (MQ=255)                                                      ccgagctcctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTa                                                                                                                                                                                                                                          <  2:385269‑M2/62‑1 (MQ=255)                                                         acaacctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtttccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTaggg                                                                                                                                                                                                                                       >  2:831319‑M2/237‑300 (MQ=255)                                                             cctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtttccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTacctcgtaagatcgg                                                                                                                                                                                                                                   >  1:385281‑M2/233‑294 (MQ=255)                                                             cctctacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGGTACCCCGTAGGAGCgg                                                                                                                                                                                                                                   >  2:37923‑M2/233‑301 (MQ=255)                                                                 cacctgcatttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGttt                                                                                                                                                                                                                               <  1:831342‑M2/73‑1 (MQ=255)                                                                   caggcatttggaagagaagaccgaaaaacggtcttgggctgccttgcagcccatacgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagaacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAc                                                                                                                                                                                                                             <  1:175805‑M2/75‑1 (MQ=255)                                                                    ctgcatttggaagagaagaccgaataacggtcttgggctgccgtgcagcccattcgcgggcaagcccgctcctgcacgtgtacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAcc                                                                                                                                                                                                                            <  1:349018‑M2/76‑1 (MQ=255)                                                                     ggaatttggaagagaagaccgaataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAccc                                                                                                                                                                                                                           <  2:316721‑M2/77‑1 (MQ=255)                                                                       catttggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCcgc                                                                                                                                                                                                                         >  1:141511‑M2/223‑301 (MQ=255)                                                                            ggaagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcCCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCCCGGAGGACCGGGTTTACCCGCCAaga                                                                                                                                                                                                                    >  2:894110‑M2/218‑301 (MQ=255)                                                                              aagagaagaccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgccaataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACCAGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAgg                                                                                                                                                                                                                  >  1:158384‑M2/216‑301 (MQ=255)                                                                                cttccagttccatatcggtcttgggctgcctggcagcccatctcggggcaagcccgctctttcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAggcc                                                                                                                                                                                                                <  2:618333‑M2/88‑1 (MQ=255)                                                                                       ccgcataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTAACTCGTACGAGCGGGTTTACCCGCGAAGAGGACGGCCcac                                                                                                                                                                                                         >  2:17323‑M2/207‑300 (MQ=255)                                                                                         ccataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGt                                                                                                                                                                                                       <  1:894133‑M2/97‑1 (MQ=255)                                                                                          cataacggtcttgggctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCCGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTAcccgcgaagaggccagatcggaag                                                                                                                                                                                                      >  1:618355‑M2/204‑291 (MQ=255)                                                                                          aataccgtccttgggctgccttgcagcccattcgcggccaggcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGtt                                                                                                                                                                                                      <  1:17325‑M2/98‑1 (MQ=255)                                                                                               gggctttgggccgccttgcagcccattggcgaccaagcccgatcctggacgtttaccggtaggggcgggcttgcttgcaatgcccccacaatccccctgccatccccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAaca                                                                                                                                                                                                 <  2:695323‑M2/103‑1 (MQ=255)                                                                                                        cctgccttgcagcccattcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCa                                                                                                                                                                                        <  1:37925‑M2/112‑1 (MQ=255)                                                                                                                       attcgcgggcaagcccgctcctgcacgtttacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCCTTACCTCGTAGGAGCCGGTTTACCCGCGAAGAAGCCGGCCCAGGTTGAACACCGGTCCCAGCTACACTGTCTTAt                                                                                                                                                                         >  1:266579‑M2/175‑301 (MQ=255)                                                                                                                                             gcacgtttacctgtagggacgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATc                                                                                                                                                   <  1:774574‑M2/149‑1 (MQ=255)                                                                                                                                                    tacctgtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACCCTGTTCTATCGAATAACCGTTGCAGTCCAACGCCCCtt                                                                                                                                            >  2:37065‑M2/146‑301 (MQ=255)                                                                                                                                                         gtaggggcgggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACAATGTCTTATCGAATAGCCGTTGCAGTCCATCGCCccttaaaaa                                                                                                                                       >  1:205175‑M2/141‑297 (MQ=255)                                                                                                                                                                 gggcttgcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGATCGGGTTTACCCGCGCAGAGGCCGGCCCCGGTTGAACACCGCTCCCAGCTACAATGtcttatcgactatccgttgccgtccttagatcgtcaggcccttgttgt                                                                                                                               >  2:472770‑M2/133‑278 (MQ=255)                                                                                                                                                                       gcttgcaatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCg                                                                                                                         <  2:141508‑M2/175‑1 (MQ=255)                                                                                                                                                                             aatgcccccaaaatccccctgccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGt                                                                                                                   <  1:37067‑M2/181‑1 (MQ=255)                                                                                                                                                                                     ccacatccccctgccatacccattcccagaacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATc                                                                                                           <  2:205170‑M2/189‑1 (MQ=255)                                                                                                                                                                                      aaaagccccctgccataccccttcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATcc                                                                                                          <  2:45084‑M2/190‑1 (MQ=255)                                                                                                                                                                                                 gccatacccattcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTGACCCGCGAAGAGGCCGGCCCAGGTTGCACAGCGCCCCCAGCTACACTCTCTTATCGAATGGCCGTTGCAGGCCATCGCCCCTTACAAGAGCAGAACAACCCGCAATGTTCGATATCCGAAAATAcccc                                                                                               >  2:736622‑M2/101‑301 (MQ=255)                                                                                                                                                                                                      ccccatacccaaaaagtggcctttgcctataatggggcaagcctgaacccggaaacgacagccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGc                                                                                          <  1:902862‑M2/206‑1 (MQ=255)                                                                                                                                                                                                          attcccagcacgtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATCTCCGCAAATACCCCCCGCCCCTg                                                                                      >  2:181532‑M2/92‑301 (MQ=255)                                                                                                                                                                                                                     gtggcctttgcctataatggtgccagcctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCCCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGCAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTAACGAATACCCGTTGCAGTCCATCGCCCCTCACAAGAGCAGGACCCCCCTCCCACTTCGATCCCCGCAAACACCCCACGTCcctggccgccccacc                                                                           >  2:247679‑M2/81‑294 (MQ=255)                                                                                                                                                                                                                                                ctgaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCCCACCCCCGAAgacca                                                >  1:859090‑M2/54‑299 (MQ=255)                                                                                                                                                                                                                                                  aaacccggcaacgactgccgtgtccaagccctcaccacgcaccgcggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTAcc                                              <  1:181536‑M2/250‑1 (MQ=255)                                                                                                                                                                                                                                                                                              cggccgcTCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGACCCAGACACCTTCTgg  >  1:667243‑M2/8‑301 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                      CGAAAAGCTGGCCCCGCACAAGGTGATCCCGGGTAACCGCCCGAGCAACACGTTGGTGGTCGAGCGCATCAGCCCGCGCCGGCTGGGTGCGCTGGTGGCCATGTACGAGCACAAGGTGTTCGTACAGAGCGTTATCTGGGGTATCAACGCCTTCGATCAGTGGGGCGTCGAGCTGGGCAAGGAGCTGGGCAAGGGCGTTTACCAGCGCTTGGTCGGTAGCCTGGAAGACAGCGCCGAAGACGGTTCCACCCAAGGCCTGATCAACTACTTCCGCGGCCGTCACCGCGGTTGATCCGAGCTTGTGCCGGCCTCTTCGCGGGTAAACCCGCCTACCAGGCAACGCGTTACCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCCAGGTTGAACACCGCTCCCAGCTACACTGTCTTATCGAATAGCCGTTGCAGTCCATCGCCCCTTACAAGAGCAGGACCACCCGCCATGTTCGATATCCGCAAATACCCCCAGGCCCTGGCCGTCAGCCAGTCCGCCGCCCTCACCCCCGAAGACTACCGCCGCCTCTACCGCCAGTCGGTCGAAGACCCAGACACCTTCTGG  >  AE015451/5344134‑5344719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑