![]() |
breseq version 0.35.5 revision 9f368df52761
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AE015451 | 1,124,554 | 1 | . | T | 56.1% | 61.3 / 62.7 | 41 | coding (589/1122 nt) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 2,550,125 | 0 | G | A | 55.8% | 13.1 / 212.5 | 163 | noncoding (1436/1522 nt) | PP_16SF | 16S ribosomal RNA |
* | AE015451 | 1,124,573 | 0 | G | A | 55.6% | ‑0.3 / 38.6 | 37 | G190G (GGC→GGT) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 699,722 | 0 | A | G | 55.3% | 6.1 / inf | 313 | intergenic (+115/‑129) | PP_t06/PP_23SD | tRNA‑Ala/23S ribosomal RNA |
* | AE015451 | 1,124,575 | 0 | C | A | 51.3% | 9.3 / 49.6 | 37 | G190C (GGC→TGC) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 196,495 | 0 | G | C | 50.7% | 178.2 / inf | 304 | T667T (ACG→ACC) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,579 | 0 | G | A | 50.0% | ‑3.5 / 41.0 | 38 | R188R (CGC→CGT) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,574 | 0 | C | A | 48.6% | 8.2 / 34.8 | 37 | G190V (GGC→GTC) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,564 | 0 | G | T | 48.4% | 24.0 / 17.3 | 37 | G193G (GGC→GGA) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 196,459 | 0 | C | A | 46.8% | 211.7 / inf | 269 | T655T (ACC→ACA) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,568 | 0 | G | T | 46.0% | 15.3 / 23.0 | 37 | T192N (ACC→AAC) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,567 | 0 | G | . | 45.2% | 43.1 / 51.8 | 37 | coding (576/1122 nt) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 4,348,705 | 0 | A | G | 45.1% | 141.5 / inf | 291 | intergenic (‑70/+404) | PP_3820/galU | Group II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase |
* | AE015451 | 1,124,577 | 0 | G | A | 44.7% | 8.1 / 30.4 | 38 | S189L (TCG→TTG) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,327,121 | 0 | C | T | 42.4% | 178.6 / inf | 256 | intergenic (+97/‑196) | PP_16SE/PP_23SE | 16S ribosomal RNA/23S ribosomal RNA |
* | AE015451 | 1,124,571 | 0 | T | A | 41.7% | 29.2 / 21.8 | 37 | Y191F (TAC→TTC) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,565 | 0 | C | G | 38.9% | 12.8 / 35.9 | 37 | G193A (GGC→GCC) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,576 | 0 | C | A | 37.8% | 38.6 / 12.9 | 37 | S189S (TCG→TCT) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 195,559 | 0 | C | A | 37.8% | 361.7 / 311.3 | 293 | T355T (ACC→ACA) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 196,438 | 0 | T | C | 37.6% | 228.1 / 242.0 | 244 | S648S (AGT→AGC) | PP_0168 | putative surface adhesion protein |
Marginal new junction evidence (lowest skew 10 of 76 shown) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AE015451 | = 4808179 | 11 (0.060) | 44 (0.250) | 32/576 | 5.3 | 87.5% | intergenic (‑288/‑267) | PP_4234/dsbD‑II | Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
? | AE015451 | 5988765 = | 2 (0.010) | intergenic (+59/‑487) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA | |||||
* | ? | AE015451 | 4808174 = | 19 (0.100) | 38 (0.230) | 26/530 | 5.9 | 39.4% | intergenic (‑283/‑272) | PP_4234/dsbD‑II | Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
? | AE015451 | = 5988935 | 100 (0.610) | intergenic (+229/‑317) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA | |||||
* | ? | AE015451 | = 1780066 | 68 (0.370) | 29 (0.160) | 25/586 | 6.7 | 31.4% | intergenic (+25/+81) | PP_1587/dapC | NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative |
? | AE015451 | 1780129 = | 60 (0.330) | intergenic (+88/+18) | PP_1587/dapC | NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative | |||||
* | ? | AE015451 | = 2957080 | 108 (0.590) | 21 (0.120) | 19/590 | 8.0 | 17.7% | intergenic (+35/‑161) | PP_2588/PP_2589 | Aminotransferase, class III/Aldehyde dehydrogenase family protein |
? | AE015451 | 2957165 = | 89 (0.490) | intergenic (+120/‑76) | PP_2588/PP_2589 | Aminotransferase, class III/Aldehyde dehydrogenase family protein | |||||
* | ? | AE015451 | 5545080 = | 166 (0.900) | 465 (2.550) | 16/594 | 8.8 | 73.9% | intergenic (‑220/+657) | rpsF/rlmB | 30S ribosomal protein S6/23S rRNA (guanosine‑2'‑O‑)‑methyltransferase RlmB |
? | AE015451 | = 5545666 | NA (NA) | intergenic (‑806/+71) | rpsF/rlmB | 30S ribosomal protein S6/23S rRNA (guanosine‑2'‑O‑)‑methyltransferase RlmB | |||||
* | ? | AE015451 | = 5201567 | 60 (0.330) | 16 (0.090) | 16/594 | 8.8 | 25.3% | intergenic (‑53/‑127) | PP_4582/PP_4583 | conserved membrane protein of unknown function/putative Peptidase |
? | AE015451 | 5201666 = | 35 (0.190) | intergenic (‑152/‑28) | PP_4582/PP_4583 | conserved membrane protein of unknown function/putative Peptidase | |||||
* | ? | AE015451 | 3826260 = | NA (NA) | 238 (1.300) | 16/596 | 8.9 | NA | intergenic (‑215/+388) | ptxS/PP_3381 | 2‑ketogluconate utilization repressor/transposase |
? | AE015451 | = 3826846 | NA (NA) | coding (1131/1329 nt) | PP_3381 | transposase | |||||
* | ? | AE015451 | = 5696567 | 56 (0.300) | 20 (0.110) | 15/584 | 9.0 | 34.6% | intergenic (+19/‑193) | pyrC'/hslV | dihydroorotase‑like protein/peptidase component of the ATP‑dependent HslVU protease |
? | AE015451 | 5696629 = | 21 (0.120) | intergenic (+81/‑131) | pyrC'/hslV | dihydroorotase‑like protein/peptidase component of the ATP‑dependent HslVU protease | |||||
* | ? | AE015451 | = 4074284 | NA (NA) | 16 (0.090) | 14/596 | 9.4 | 8.2% | coding (1131/1329 nt) | PP_3586 | transposase |
? | AE015451 | 4302323 = | 180 (0.980) | intergenic (+813/‑487) | PP_3774/PP_5627 | conserved protein of unknown function/protein of unknown function | |||||
* | ? | AE015451 | = 2958771 | 82 (0.450) | 9 (0.050) | 9/588 | 11.1 | 9.9% | intergenic (+37/+105) | PP_2589/PP_2590 | Aldehyde dehydrogenase family protein/Outer membrane ferric siderophore receptor |
? | AE015451 | 2958833 = | 84 (0.470) | intergenic (+99/+43) | PP_2589/PP_2590 | Aldehyde dehydrogenase family protein/Outer membrane ferric siderophore receptor |