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breseq version 0.35.5 revision 9f368df52761
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AE015451 | 2,550,125 | 0 | G | A | 66.7% | 47.1 / 106.3 | 118 | noncoding (1436/1522 nt) | PP_16SF | 16S ribosomal RNA |
* | AE015451 | 699,722 | 0 | A | G | 58.9% | 49.4 / 286.4 | 238 | intergenic (+115/‑129) | PP_t06/PP_23SD | tRNA‑Ala/23S ribosomal RNA |
* | AE015451 | 196,495 | 0 | G | C | 52.3% | 40.0 / 291.3 | 172 | T667T (ACG→ACC) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,554 | 1 | . | T | 51.1% | 72.4 / 84.9 | 47 | coding (589/1122 nt) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,577 | 0 | G | A | 48.8% | 11.8 / 36.5 | 41 | S189L (TCG→TTG) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 4,348,705 | 0 | A | G | 48.3% | 48.1 / 270.8 | 176 | intergenic (‑70/+404) | PP_3820/galU | Group II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase |
* | AE015451 | 196,459 | 0 | C | A | 47.6% | 108.7 / 244.6 | 171 | T655T (ACC→ACA) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,579 | 0 | G | A | 47.6% | 5.3 / 43.9 | 42 | R188R (CGC→CGT) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 196,438 | 0 | T | C | 46.3% | 89.1 / 229.0 | 170 | S648S (AGT→AGC) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,568 | 0 | G | T | 46.3% | 27.6 / 28.4 | 41 | T192N (ACC→AAC) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,573 | 0 | G | A | 46.3% | 5.2 / 44.2 | 41 | G190G (GGC→GGT) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,574 | 0 | C | A | 46.3% | 12.3 / 34.5 | 41 | G190V (GGC→GTC) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,564 | 0 | G | T | 46.2% | 20.6 / 28.3 | 41 | G193G (GGC→GGA) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,567 | 0 | G | . | 46.2% | 61.6 / 68.0 | 41 | coding (576/1122 nt) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 196,579 | 0 | G | A | 45.2% | 52.8 / 203.7 | 165 | V695V (GTG→GTA) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,575 | 0 | C | A | 43.9% | 24.5 / 32.5 | 41 | G190C (GGC→TGC) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,576 | 0 | C | A | 43.9% | 25.5 / 28.6 | 41 | S189S (TCG→TCT) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,565 | 0 | C | G | 42.5% | 18.4 / 42.9 | 41 | G193A (GGC→GCC) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,327,121 | 0 | C | T | 41.7% | 165.5 / 244.9 | 199 | intergenic (+97/‑196) | PP_16SE/PP_23SE | 16S ribosomal RNA/23S ribosomal RNA |
* | AE015451 | 1,124,571 | 0 | T | A | 39.0% | 39.6 / 22.6 | 41 | Y191F (TAC→TTC) | gcvT‑I | aminomethyltransferase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AE015451 | 4808174 = | 0 (0.000) | 23 (0.230) | 19/530 | 3.8 | 40.7% | intergenic (‑283/‑272) | PP_4234/dsbD‑II | Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
? | AE015451 | = 5988935 | 67 (0.680) | intergenic (+229/‑317) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA | |||||
* | ? | AE015451 | 5545080 = | 130 (1.170) | 343 (3.120) | 21/594 | 3.9 | 72.7% | intergenic (‑220/+657) | rpsF/rlmB | 30S ribosomal protein S6/23S rRNA (guanosine‑2'‑O‑)‑methyltransferase RlmB |
? | AE015451 | = 5545666 | NA (NA) | intergenic (‑806/+71) | rpsF/rlmB | 30S ribosomal protein S6/23S rRNA (guanosine‑2'‑O‑)‑methyltransferase RlmB | |||||
* | ? | AE015451 | 3826260 = | NA (NA) | 206 (1.870) | 21/596 | 3.9 | NA | intergenic (‑215/+388) | ptxS/PP_3381 | 2‑ketogluconate utilization repressor/transposase |
? | AE015451 | = 3826846 | NA (NA) | coding (1131/1329 nt) | PP_3381 | transposase | |||||
* | ? | AE015451 | = 4074284 | NA (NA) | 20 (0.180) | 18/596 | 4.4 | 16.2% | coding (1131/1329 nt) | PP_3586 | transposase |
? | AE015451 | 4302323 = | 104 (0.940) | intergenic (+813/‑487) | PP_3774/PP_5627 | conserved protein of unknown function/protein of unknown function | |||||
* | ? | AE015451 | 2837080 = | 22 (0.200) | 13 (0.170) | 11/416 | 4.5 | 63.0% | intergenic (‑32/+151) | sad‑I/PP_2489 | NAD+‑dependent succinate semialdehyde dehydrogenase/putative Xenobiotic reductase |
? | AE015451 | = 2837063 | 0 (0.000) | intergenic (‑15/+168) | sad‑I/PP_2489 | NAD+‑dependent succinate semialdehyde dehydrogenase/putative Xenobiotic reductase | |||||
* | ? | AE015451 | = 5696567 | 20 (0.180) | 13 (0.120) | 11/584 | 5.9 | 52.6% | intergenic (+19/‑193) | pyrC'/hslV | dihydroorotase‑like protein/peptidase component of the ATP‑dependent HslVU protease |
? | AE015451 | 5696629 = | 4 (0.040) | intergenic (+81/‑131) | pyrC'/hslV | dihydroorotase‑like protein/peptidase component of the ATP‑dependent HslVU protease | |||||
* | ? | AE015451 | = 1780066 | 38 (0.340) | 9 (0.080) | 9/586 | 6.5 | 19.5% | intergenic (+25/+81) | PP_1587/dapC | NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative |
? | AE015451 | 1780129 = | 37 (0.340) | intergenic (+88/+18) | PP_1587/dapC | NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative | |||||
* | ? | AE015451 | = 5201567 | 44 (0.400) | 11 (0.100) | 8/594 | 6.9 | 23.5% | intergenic (‑53/‑127) | PP_4582/PP_4583 | conserved membrane protein of unknown function/putative Peptidase |
? | AE015451 | 5201666 = | 28 (0.250) | intergenic (‑152/‑28) | PP_4582/PP_4583 | conserved membrane protein of unknown function/putative Peptidase |