breseq  version 0.35.5  revision 9f368df52761
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451699,7220AG58.4% 6.2 / 151.3 125intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*AE0154512,550,1250GA52.2% 20.6 / 98.7 67noncoding (1436/1522 nt)PP_16SF16S ribosomal RNA
*AE015451196,4950GC45.4% 153.9 / 246.5 153T667T (ACG→ACCPP_0168putative surface adhesion protein
*AE0154511,124,5541.T42.9% 23.1 / 43.7 22coding (589/1122 nt)gcvT‑Iaminomethyltransferase
*AE015451196,4590CA41.2% 192.8 / 154.6 133T655T (ACC→ACAPP_0168putative surface adhesion protein
*AE0154511,327,1210CT39.2% 78.5 / 113.9 102intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*AE015451196,5790GA38.2% 145.4 / 154.9 146V695V (GTG→GTAPP_0168putative surface adhesion protein
*AE015451196,4380TC37.2% 146.8 / 127.8 126S648S (AGT→AGCPP_0168putative surface adhesion protein
*AE0154511,124,5650CG34.6% 32.4 / 20.1 26G193A (GGC→GCC) gcvT‑Iaminomethyltransferase
*AE0154511,124,5670G.34.6% 52.9 / 29.2 26coding (576/1122 nt)gcvT‑Iaminomethyltransferase
*AE0154511,124,5680GT34.6% 34.5 / 13.1 26T192N (ACC→AAC) gcvT‑Iaminomethyltransferase
*AE015451195,5590CA33.6% 217.8 / 92.2 110T355T (ACC→ACAPP_0168putative surface adhesion protein
*AE0154511,124,5740CA33.3% 47.3 / 13.6 30G190V (GGC→GTC) ‡gcvT‑Iaminomethyltransferase
*AE0154511,124,5750CA33.3% 48.7 / 11.8 30G190C (GGC→TGC) ‡gcvT‑Iaminomethyltransferase
*AE0154514,348,7050AG31.7% 121.2 / 90.7 104intergenic (‑70/+404)PP_3820/galUGroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*AE015451195,6340CT29.2% 136.0 / 80.9 113T380T (ACC→ACTPP_0168putative surface adhesion protein
*AE0154511,124,5790GA29.0% 42.5 / 14.0 32R188R (CGC→CGTgcvT‑Iaminomethyltransferase
*AE0154511,124,5770GA28.1% 45.4 / 12.6 32S189L (TCG→TTG) gcvT‑Iaminomethyltransferase
*AE0154511,124,5730GA26.7% 39.4 / 14.9 30G190G (GGC→GGT) ‡gcvT‑Iaminomethyltransferase
*AE015451196,8790GA25.9% 204.5 / 104.5 159V795V (GTG→GTAPP_0168putative surface adhesion protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451 = 48081790 (0.000)18 (0.270) 11/576 3.5 100% intergenic (‑288/‑267) PP_4234/dsbD‑II Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2
?AE015451 5988765 = 0 (0.000)intergenic (+59/‑487) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? AE015451 5545080 =70 (1.000)237 (3.410) 10/594 3.9 77.4% intergenic (‑220/+657) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine‑2'‑O‑)‑methyltransferase RlmB
?AE015451 = 5545666 NA (NA)intergenic (‑806/+71) rpsF/rlmB 30S ribosomal protein S6/23S rRNA (guanosine‑2'‑O‑)‑methyltransferase RlmB
* ? AE015451 = 569656715 (0.210)5 (0.070) 5/584 5.4 32.7% intergenic (+19/‑193) pyrC'/hslV dihydroorotase‑like protein/peptidase component of the ATP‑dependent HslVU protease
?AE015451 5696629 = 6 (0.090)intergenic (+81/‑131) pyrC'/hslV dihydroorotase‑like protein/peptidase component of the ATP‑dependent HslVU protease