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breseq version 0.35.5 revision 9f368df52761
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AE015451 | 699,722 | 0 | A | G | 58.4% | 6.2 / 151.3 | 125 | intergenic (+115/‑129) | PP_t06/PP_23SD | tRNA‑Ala/23S ribosomal RNA |
* | AE015451 | 2,550,125 | 0 | G | A | 52.2% | 20.6 / 98.7 | 67 | noncoding (1436/1522 nt) | PP_16SF | 16S ribosomal RNA |
* | AE015451 | 196,495 | 0 | G | C | 45.4% | 153.9 / 246.5 | 153 | T667T (ACG→ACC) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,554 | 1 | . | T | 42.9% | 23.1 / 43.7 | 22 | coding (589/1122 nt) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 196,459 | 0 | C | A | 41.2% | 192.8 / 154.6 | 133 | T655T (ACC→ACA) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,327,121 | 0 | C | T | 39.2% | 78.5 / 113.9 | 102 | intergenic (+97/‑196) | PP_16SE/PP_23SE | 16S ribosomal RNA/23S ribosomal RNA |
* | AE015451 | 196,579 | 0 | G | A | 38.2% | 145.4 / 154.9 | 146 | V695V (GTG→GTA) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 196,438 | 0 | T | C | 37.2% | 146.8 / 127.8 | 126 | S648S (AGT→AGC) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,565 | 0 | C | G | 34.6% | 32.4 / 20.1 | 26 | G193A (GGC→GCC) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,567 | 0 | G | . | 34.6% | 52.9 / 29.2 | 26 | coding (576/1122 nt) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,568 | 0 | G | T | 34.6% | 34.5 / 13.1 | 26 | T192N (ACC→AAC) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 195,559 | 0 | C | A | 33.6% | 217.8 / 92.2 | 110 | T355T (ACC→ACA) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,574 | 0 | C | A | 33.3% | 47.3 / 13.6 | 30 | G190V (GGC→GTC) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,575 | 0 | C | A | 33.3% | 48.7 / 11.8 | 30 | G190C (GGC→TGC) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 4,348,705 | 0 | A | G | 31.7% | 121.2 / 90.7 | 104 | intergenic (‑70/+404) | PP_3820/galU | Group II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase |
* | AE015451 | 195,634 | 0 | C | T | 29.2% | 136.0 / 80.9 | 113 | T380T (ACC→ACT) | PP_0168 | putative surface adhesion protein |
* | AE015451 | 1,124,579 | 0 | G | A | 29.0% | 42.5 / 14.0 | 32 | R188R (CGC→CGT) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,577 | 0 | G | A | 28.1% | 45.4 / 12.6 | 32 | S189L (TCG→TTG) | gcvT‑I | aminomethyltransferase |
* | AE015451 | 1,124,573 | 0 | G | A | 26.7% | 39.4 / 14.9 | 30 | G190G (GGC→GGT) ‡ | gcvT‑I | aminomethyltransferase |
* | AE015451 | 196,879 | 0 | G | A | 25.9% | 204.5 / 104.5 | 159 | V795V (GTG→GTA) | PP_0168 | putative surface adhesion protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AE015451 | = 4808179 | 0 (0.000) | 18 (0.270) | 11/576 | 3.5 | 100% | intergenic (‑288/‑267) | PP_4234/dsbD‑II | Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
? | AE015451 | 5988765 = | 0 (0.000) | intergenic (+59/‑487) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA | |||||
* | ? | AE015451 | 5545080 = | 70 (1.000) | 237 (3.410) | 10/594 | 3.9 | 77.4% | intergenic (‑220/+657) | rpsF/rlmB | 30S ribosomal protein S6/23S rRNA (guanosine‑2'‑O‑)‑methyltransferase RlmB |
? | AE015451 | = 5545666 | NA (NA) | intergenic (‑806/+71) | rpsF/rlmB | 30S ribosomal protein S6/23S rRNA (guanosine‑2'‑O‑)‑methyltransferase RlmB | |||||
* | ? | AE015451 | = 5696567 | 15 (0.210) | 5 (0.070) | 5/584 | 5.4 | 32.7% | intergenic (+19/‑193) | pyrC'/hslV | dihydroorotase‑like protein/peptidase component of the ATP‑dependent HslVU protease |
? | AE015451 | 5696629 = | 6 (0.090) | intergenic (+81/‑131) | pyrC'/hslV | dihydroorotase‑like protein/peptidase component of the ATP‑dependent HslVU protease |